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51.
Immobilizers are described that have been used during computed tomography (CT) to ensure reproducibility of patient position during all phases of radiation treatment planning and delivery. The immobilizer causes no degradation or artifact on CT images; hence, the value of the images in radiation treatment planning is not compromised. 相似文献
52.
S.K. GUPTA OM SINGH G.P. TALWAR 《American journal of reproductive immunology (New York, N.Y. : 1989)》1985,7(3):104-108
ABSTRACT: Mouse hybrid cell clones secreting antigonadotropin releasing hormone monoclonal antibody were developed by fusion of SP2/O-Ag 1.4 myeloma cells with splenocytes of mouse immunized with gonadotropin releasing hormone (GnRH) tagged to tetanus toxoid. The product of hybrid cell clones obtained as ascites fluid from mouse peritoneal cavity had a titre of 106 (30–40% binding of 125I-GnRH) in radioimmunoassay. The antibody was IgG2a and Kappa. The association constant (Ka) of the product of hybrid cell clone P81662 for binding with GnRH was 1.2 × 109 L/mole. The monoclonal antibody (P81662) was highly specific for the native GnRH and devoid of reactivity with thyroid releasing hormone as tested in competitive radioimmunoassay. The recognition for GnRH agonists by monoclonal was 387-fold less with D-Ser (But)6 des Gly10 GnRH ethylamide and 608-fold less with Bz1-His6 GnRH. Monoclonal anti-GnRH antibody was competent to neutralize the in vivo bioactivity of the hormone as evident by the block of estrus cycle and termination of pregnancy in mice. Termination of pregnancy in animals receiving anti-GnRH monoclonal could be prevented by administration of progesterone. 相似文献
53.
54.
Hck expression correlates with granulocyte-macrophage colony- stimulating factor-induced proliferation in HL-60 cells 总被引:2,自引:0,他引:2
The human myeloid cell line HL-60 expresses approximately 300 high- affinity granulocyte-macrophage colony-stimulating factor receptors (GM- CSFRs), yet treatment of these cells with GM-CSF does not result in enhanced cellular proliferation or increases in protein tyrosine phosphorylation. In contrast, GM-CSF induces rapid increases in protein tyrosine phosphorylation and proliferative responses in HL-60 cells pretreated for 3 days in dimethyl sulfoxide (DMSO). Similarly, HL-60 cells pretreated with retinoic acid or 1,25 dihydroxyvitamin D3 were also capable of responding to GM-CSF. Interestingly, each of these treatments resulted in increased expression of the src-like tyrosine kinase hck. Stimulation with GM-CSF increased hck autophosphorylation in DMSO-treated HL-60 cells, suggesting that hck is a component of the GM-CSF signal transduction pathway. To determine if hck has a role in the DMSO-induced recoupling of the GM-CSFR, we overexpressed hck in HL- 60 cells. The resulting cell line (HL-60/hck) expresses hck mRNA and protein at levels comparable with DMSO-treated HL-60 cells. Stimulation of HL-60/hck cells with GM-CSF results in activation of hck, increases in protein tyrosine phosphorylation, and increased proliferation. These results show that cytokine receptors can exist in an uncoupled form and suggest that in HL-60 cells, appropriate levels of the src-like tyrosine kinase hck are critical for functional coupling of the GM-CSFR to biologic responses. 相似文献
55.
A simplified serum-free method for preparation and cultivation of human granulosa-luteal cells 总被引:1,自引:0,他引:1
Figenschau Y; Sundsfjord JA; Yousef MI; Fuskevag OM; Sveinbjornsson B; Bertheussen K 《Human reproduction (Oxford, England)》1997,12(3):523-531
A simplified method for the preparation and long-term cultivation of
granulosa-luteal cells in serum-free medium is described. The cells were
harvested from women undergoing in-vitro fertilization, enriched by
sedimentation and dissociated by enzymatic treatment. We demonstrated, by
introducing a synthetic serum replacement (SSR2), that these primary cell
cultures cultivated in monolayers on an extracellular matrix may be used in
experiments exceeding 7 days with low cell loss and cell death. No adverse
effect on progesterone production was found. There was a high diversity in
progesterone production between cells from individual patients. After
several days in culture, the cells were challenged with human chorionic
gonadotrophin which revived the rapidly decreasing progesterone production.
We were unable to demonstrate an increase in cell number after 7 days of
cultivation when the cells were grown in medium supplemented with either
serum or SSR2. The mitogens epidermal growth factor and basic fibroblast
growth factor had no influence on proliferation. We also found that the
present method prevents leukocyte contamination in the granulosa-luteal
cell cultures. Compared with the common method based on the enrichment of
granulosa-luteal cells on a density gradient (Ficoll/Percoll), this method
saves time, labour and expense, in addition to augmenting purity.
相似文献
56.
Antibody-induced antigenic modulation (AIAM) of CD10 and CD19 was studied on NALM-6, RAJI, and JOK-1 cell lines using fluorescence microscopy (FM), flow cytometry (FCM), and immunoelectron microscopy (IEM). Cross-linking with monoclonal antibodies (MoAbs) induced rapid redistribution of CD10 and CD19 on the cell surface (FM) followed by internalization involving uptake through plasmalemmal pits, transfer through endosomal compartment (receptor-mediated endocytosis), and, finally, delivery to lysosomes for degradation or exocytosis and recycling (IEM). Significant quantitative differences regarding modulation and intracellular processing were shown by FCM and IEM. Thus, 35%, 30%, and 25% of CD10 compared with 80%, 60%, and 40% of CD19 were internalized in NALM-6, RAJI, and JOK-1 cells, respectively. Also, the rate of intracellular transfer as well as externalization and recycling was more pronounced in the case of CD19 than of CD10 and in the NALM-6 and RAJI cells compared with the JOK-1 cells. These differences may possibly reflect the functional significance of CD10 and CD19 as well as the stage of differentiation of the malignant B cells. Although both antigens can be useful in MoAb-targeted immunotherapy, our findings suggest that anti-CD19 MoAbs would be preferable for delivery of cytotoxic agents to malignant B cells. 相似文献
57.
Objective
To study detail pharmacognosy and anti-inflammatory activity of Callistemon lanceolatus (C. lanceolatus) leaf.Methods
Leaf sample was studied by organoleptic, macroscopical, microscopical, phytochemical and other WHO recommended methods for standardizations. The methanolic leaf extract of the plant was also screened for anti-inflammatory activity on carrageenan-induced paw edema in rat at doses of 200 and 400 mg/kg, orally. The detail pharmacognostic study of the C. lanceolatus leaf was carried out to lay down the standards which could be useful in future experimental studies.Results
C. lanceolatus methanolic leaf extract showed significant (P<0.05) anti-inflammatory activity at doses of 200 mg/kg and 400 mg/kg. This significant anti-inflammatory of C. lanceolatus methanolic leaf extract at the dose of 400 mg/kg was comparable with diclofenac sodium.Conclusions
The pharmacognostic profile of the C. lanceolatus leaf is helpful in standardization for quality, purity and sample identification. The methanolic extract at a dose of 400 mg/kg shows a significant activity in comparison with the standard drug diclofenac sodium (50 mg/kg). 相似文献58.
NM Mc Loone† J Tolland† M Walsh‡ OM Dolan† 《Journal of the European Academy of Dermatology and Venereology》2006,20(6):698-701
BACKGROUND: Follow-up of patients after treatment of basal cell carcinoma (BCC) allows for monitoring of recurrence and detection of new tumours, but adds a significant burden to outpatient clinics. At the skin tumour clinic in the dermatology department, the Royal Hospitals, Belfast, all patients are reviewed for 2 years after surgical excision of a low-risk primary BCC. OBJECTIVES: An audit was undertaken to determine the quality of data set recorded relating to prognostic factors for BCCs to determine the rate of recurrence and number of new primary tumours detected and to determine the completeness of follow-up by patients. METHOD: Patients who had primary BCCs treated by excision were identified from a database held at the clinic. Medical charts were reviewed to determine data recorded about lesions, the number of recurrent BCCs and new tumours detected, and the number of patients completing follow-up. RESULTS: Between January 1999 and December 2000, 114 patients had 121 primary BCCs excised. BCC location and size were recorded in 100% and 35% of cases, respectively. Histological type was stated for morphoeic or multifocal lesions. Two years of follow-up was completed by 53% of patients and 1 year by 78% of patients. The rate of recurrence was low, with 2 BCC recurring within 2 years of excision. The risk of developing a new BCC was 11.6% in the first year and 6.3% in the second year. CONCLUSIONS: Follow-up of patients after excision of a low-risk BCC at the clinic has been reduced to 1 year. A proforma has been developed to encourage documentation of prognostic factors. 相似文献
59.
60.