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Primary melanoma cells of the vertical growth phase: similarities to metastatic cells 总被引:7,自引:0,他引:7
M Herlyn G Balaban J Bennicelli D Guerry R Halaban D Herlyn D E Elder G G Maul Z Steplewski P C Nowell 《Journal of the National Cancer Institute》1985,74(2):283-289
Three primary and 16 metastatic melanoma cell lines were established from primary and metastatic lesions of 4 patients with malignant melanoma. Comparison of metastatic melanoma cells with cells of the vertical growth phase (VGP) or late primary melanoma from the same individual revealed, generally, a shorter population-doubling time, growth to a higher cell density, higher tyrosinase activity, and more pigmentation in metastatic cells. Conversely, primary and metastatic melanoma cells had similar morphology, plating efficiency, and tumorigenicity in nude mice. Karyotypic analysis revealed clonality and nonrandom abnormalities in chromosomes 1, 6, and 7 in cells of the primary and metastatic lesions of the 3 patients studied. Few differences were found in the expression of melanoma-associated antigens on short-term and long-term cultured cells by tests with monoclonal antibodies in mixed hemadsorption assays, flow cytometry, and radioimmunoassays. Our results indicate that cells cultured from the VGP but not from the radial growth phase of primary melanoma are similar to metastatic melanoma cells. 相似文献
997.
Mota HC; Vijayakumar S; Sibata C; Higgins PD; Thomas FJ; Saxton J; Weinstein M 《Radiology》1988,168(3):859-861
To determine the optimal width of a midline posterior spinal block (MPSB) (to avoid delivering too great a dose to the cord and too small a dose to adjacent tissue), the authors determined with magnetic resonance (MR) imaging normal ranges of cord depth and width and correlated them with film dosimetric data. In 59 randomly selected patients there was a wide range for both depth and width. The average depths of the anterior and posterior surfaces of the cord were 6.7 cm +/- 1.4 and 5.4 cm +/- 1.3, respectively. The average cord width was 1.6 cm +/- 0.4. Optimal cord block width as a function of cord width was determined for a 6-MV photon beam. The optimal cord block width at the surface (half-value layer [HVL] thickness = 6) varied from 1.5 to 3.0 cm for cord widths of 0.8-2.4 cm, which correspond to two standard deviations from the average. There was no significant dependence on depth of the cord. For optimal treatment outcome, the MPSB width may have to be determined for each patient individually. 相似文献
998.
ELLEN S. PARHAM PD RD 《Journal of obstetric, gynecologic, and neonatal nursing : JOGNN / NAACOG》1974,3(3):58-61
Early influences associated with adult obesity include overfeeding leading to fat cell hyperplasia, inactivity, and faulty mechanisms of control of food intake. The first year of life is critical for the intiation of these influences; thus we need to reevaluate some of the approaches to infant feeding. Excessive weight gains in infants must be taken seriously. This article reviews related literature and makes recommendations for preventing early obesity. 相似文献
999.
W. Hinterberger PD Dr. W. R. Paukovits H. Kinast E. Moritz O. Zwinz 《Annals of hematology》1978,37(2):69-74
Summary Medium conditioned by human lung tissue was found to contain colony stimulating activity (CSA). This material was tested against mouse and human bone marrow as target system. Colony forming units (CFUc)from both species responded and gave rise to clonal growth in agar cultures. This colony formation was dose dependent and the relationship was a sigmoid one. Experiments to determine the molecular weight of human lung derived colony stimulating Factors brought evidence for four active molecular weight fractions with approximately 79000, 40000, 23000 and 2000 daltons. The 23000 dalton fraction activated human cells only, whereas the other fractions were active on both human and mouse bone marrow cells. 相似文献
1000.