乳猪肝制备肝水解肽的工艺研究

目的以乳猪肝为原料制备肝水解肽，分析产品活性。方法应用酶水解／超滤法提纯制备肝水解肽，采用Folin-酚法、四氮甲唑蓝（MTT）法测定含量及活性，并与成年动物肝脏对比。结果乳猪肝组收率明显高于成年动物肝组，产品活性与后者的比较，极显著性差异。结论采用乳猪肝制备肝水解肽，产品的生物活性高，收率好，具有一定的应用价值？     

非小细胞肺癌survivin和Flk1表达及关系分析

目的:观察survivin蛋白和血管内皮细胞生长因子(VEGF)受体FIk1在不同病理类型及不同临床分期的非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)中的表达规律并探讨其相互关系。方法:采用临床NSCLC患者病理标本进行研究,NSCLC组为56例非小细胞肺癌肿瘤病理标本,患者临床资料分类中,临床Ⅰ期15例、Ⅱ期28例、Ⅲ期13例,病理分级分类中高分化肿瘤16例、中分化肿瘤22例、低分化肿瘤18例,对照组为18例癌旁组织。采用SABC法进行survivin蛋白和FIk1蛋白表达的免疫组织化学检测,分析染色结果确定阳性和阴性。结果:56例NSCLC中44例survivin表达阳性,survivin阳性率为78.6%(44/56),显著高于癌旁组。survivin阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。56例NSCLC中42例FIk1表达阳性,占75.0%(42/56),显著高于癌旁组。FIk1阳性表达在不同NSCLC肿瘤病理分级中相差显著,在不同临床分期、不同淋巴结转移中显著不同。NSCLC中survivin与FIk1阳性表达显著相关。结论:Survivin和FIk1共同高表达与NSCLC发生发展及浸润转移有显著关系。     

成年功能性构音障碍患者辅音错误类型及方式分析

目的 探讨成年功能性构音障碍患者辅音发音错误的特点,为制定语音训练方案提供依据.方法以42例成年功能性构音障碍患者为研究对象,借助计算机语音工作站对患者进行语音评估,对其辅音的发音错误类型及方式进行分析.结果42例成年功能性构音障碍患者主要的辅音发音错误类型为置换、扭曲,其次为脱落.按发音部位,错误辅音依次为舌尖后音(39例,92.86%)、舌尖前音(31例,73.81%)、舌面音(22例,52.38%)、舌根音(19例,45.24%)、舌尖中音(18例,42.86%)、唇齿音(6例,14.29%)及双唇音(6例,14.29%);辅音错误频率由高至低依次为/sh/、/zh/、/ch/、/r/、/z/、/c/、/s/、/q/、/x/、/j/、/g/、/k/、/h/、/t/、/l/、/d/、/f/、/p/.辅音错误方式有:舌前音化(22例,52.38%)、非送气化(12例,28.57%)、侧化构音(12例,28.57%)、辅音脱落(9例21.42%)、舌后音化(8例,19.05%)、舌面音化(4例,9.52%)、双唇音化(4例,9.52%)、唇齿音化(3例,7.14%).结论成年功能性构音障碍患者的辅音发音错误类型主要是置换和扭曲;错误辅音主要为舌尖后音、舌尖前音、舌面音、舌根音、舌尖中音;辅音发音错误方式主要为舌前音化、非送气化、侧化构音、辅音脱落.     

Prevention and control strategies in the diagnosis and treatment of solid tumors in children during the COVID-19 pandemic

Abstract

Since the emergence of patients with COVID-19 in December 2019, the virus has rapidly spread worldwide. Children with malignant tumors are more prone to be infected and develop severe infections. Prevention and control of the pandemic and ensuring the progress of the cancer diagnosis and treatment is a significant problem in the current scenario. This article proposes a scientific management system for patients with solid tumors to guarantee emergency surgery, rationally arrange limited-term surgery, appropriately defer elective surgery, and guarantee regular chemotherapy, while protecting children from SARS-CoV-2 infection and ensuring the continuity of comprehensive diagnosis and treatment.     

参芍降脂片对高脂血症小鼠血脂水平及肝功能的影响

目的:探讨参芍降脂片对蛋黄乳剂腹腔注射致急性高脂血症模型小鼠的血脂水平及肝功能指标的影响。方法:采用75%蛋黄乳剂腹腔注射建立急性高脂血症模型,连续灌胃给予参芍降脂片11天,末次给药2h后,除正常对照组外其余各组腹腔注入新鲜蛋黄乳剂0.1ml/10g,20h后摘眼球取血,检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量。结果:参芍降脂片300mg/kg可降低小鼠血清TC、TG、LDL-C、ALT、AST,升高HDL-C/TC比值;参芍降脂片150mg/kg可显著升高小鼠血清HDL-C/TC;参芍降脂片75mg/kg可显著降低小鼠血清TC、TG、LDL-C、ALT、AST,升高HDL-C/TC比值。结论:参芍降脂片能改善蛋黄乳剂致急性高脂血症模型小鼠的血脂水平及肝功能。     

痛风颗粒质量标准的研究

目的:建立痛风颗粒的质量标准。方法:采用薄层色谱法对方中牛蒡子、威灵仙等进行了鉴别;用高效液相色谱法测定方中牛蒡子苷的含量。结果:薄层鉴别专属性强;牛蒡子苷线性范围为0.96-4.8μg(r=0.9998),平均回收率为98.59%,RSD值为1.80%(n=6)。结论:所建立的方法操作简单,结果准确,灵敏度高,重现性好。该质量标准能有效地控制痛风颗粒的质量。     

CCL_4诱导大鼠肝损伤模型的代谢组学及茵陈蒿汤的干预作用研究

基于代谢组学的系统化理论,以UPLC/QTOF分析系统为核心手段,结合生物样品分析制备方法,研究CCL_4诱导肝损伤大鼠的代谢组变化,以及茵陈蒿汤干预后的肝损伤大鼠代谢组的回调趋势。同时,借助MS解析理论和主成分分析软件,明晰表征大鼠肝损伤的5个内源性特征生物标记物,并结合系统生物学的理论还原肝损伤的机理与实质,以及茵陈蒿汤的肝损伤防治机理,从药物代谢组学角度对经典方剂防治肝损伤给出全新解释。     

Lifespan extension by n-butanol extract from seed of Platycladus orientalis in Caenorhabditis elegans

Aim of the study

As a traditional Chinese medicine, seed of Platycladus orientalis(Linnaeus) Franco has been extensively used as a tonic and sedative remedy. The present study was conducted to investigate whether lifespan was extended and the mechanisms of n-butanol extract from seed of Platycladus orientalis (BSPO) in Caenorhabditis elegans. The findings could provide the pharmacological basis for a treatment in traditional medicine.

Materials and methods

Lifespan extension by BSPO was evaluated under normal culture conditions and in a stress test. A possible mechanism of the anti-aging effect of BSPO, a change in the stress-resistance of related proteins, was also investigated in C. elegans.

Results

It has been shown that BSPO could significantly extend lifespan of C. elegans in a concentration dependent manner under normal culture conditions and stress. Further studies demonstrated that BSPO treatment significantly decreased reactive oxygen species (ROS) accumulation, up-regulated resistance to stress of related proteins, including glutathione S-transferase-4 (GST-4) and heat shock protein-16.2 (HSP-16.2), and reduced the amount of lipofuscin in transgenic C. elegans.

Conclusion

These results indicated that BSPO extended the lifespan, which could be attributed to its direct ROS scavenging activity, reducing the amount of lipofuscin and increasing the expression of gens associated with resistance to stress. These obtained data provided valuable support for traditional clinical practice to extend lifespan and to provide tonic remedy.