正常大鼠和人肺组织一氧化氮合成酶组织化学定位研究

以还原型辅酶Ⅱ-黄递酶组织化学法对大鼠和人肺组织内一氧化氮合成酶(NOS)进行定位研究.结果显示:NOS广泛分布于各级支气管、肺泡管和部分肺泡囊上皮细胞及肺血管内皮细胞内.但肺泡及血管中、外膜NOS呈阴性反应,且NOS在大鼠和人肺组织内分布无明显区别.从而提示一氧化氮在肺组织的生理和病理学功能上可能发挥着重要作用.     

The Different Expression of PPARγ in the Artery Tissues of Hypertensive Patients with Different Ages

Objective： To study the expression of the peroxisome proliferator-activated receptor γ（PPARγ） in the artery tissues of essential hypertensive patients, and the different changes with different ages, especially to the hypertensive patients more than 65 years old. Methods： Collected the mesenteric artery tissues of essential hypertensive patients（ 〉 65 years old group and 〈 65 years old group）and patients with normal blood pressure, using immunohistochemical analysis and image acquiring and analysis system to detect the expression of PPARγ in the artery tissues. Results： the expression of PPARγ in the artery tissues of essential hypertensive patients is higher than that in the patients with normal blood pressure（ P 〈 0.05）, and to the group of hypertensive patients, the expression of PPARγ in 〉 65 years old group is higher than that in 〈65 years old group （P〈0.05）. Conclusion： the expression of PPARγ in artery tissues is increased in hypertensive patients than in the patients with normal blood pressure, and increased with aging in hypertensive patients, suggesting that PPARγ has great relationship with hypertension.     

原发性肾病综合征患者体内氧化及抗氧化系统改变的探讨

目的 :探讨原发性肾病综合征 (PNS)患者体内氧化损伤状态和抗氧化系统的改变。方法 :测定了 38例肾病综合征患者的血清丙二醛 (MDA)、谷胱甘肽过氧化物酶 (GSH -PX)及血清和红细胞超氧化物歧化酶(SOD)的含量 ,并与正常对照组进行了比较。结果 :38例PNS患者血清丙二醛水平明显高于正常对照组 ,有显著性差异 (P <0 .0 1) ;GSH -PX、血清SOD ,红细胞SOD及SOD/MDA均明显低于正常对照组 ,有显著性差异 (P <0 .0 1)。结论 :脂质过氧化物参与了肾病综合征的致病 ,以及肾病综合征患者抗氧化能力下降导致了肾病综合征患者的发病。     

肿瘤侵袭转移过程中基质金属蛋白酶作用机制系列研究

针对肿瘤侵袭转移过程中基质金属蛋白酶(MMPs)及其抑制物的作用和调节机制进行了较深入的系列研究。在包括肺癌、前列腺癌和黑色素瘤的几组具有不同转移潜能的人肿瘤细胞系中，癌细胞产生MMP-2和MMP-9的能力与其侵袭转移能力密切相关。进一步通过反义基因转染技术证实，抑制MMP-9基因表达可以明显降低高转移癌细胞的体外侵袭及裸鼠体内自发转移能力。将金属蛋白酶组织抑制因子TIMP-1，TIMP-2和TIMP-3基因分别导入高转移癌细胞，可以观察到转染细胞的侵袭转移能力受到明显抑制。说明通过选择性抑制MMPs基因表达或增强TIMPs基因表达均可在一定程度上达到抑制肿瘤侵袭转移的目的。与此同时，我们应用定时控制表达技术分别将正义或反义MMP-9基因导入MMP-9不表达或高表达的黑色素瘤细胞，通过四环素控制的分子开关成功实现了MMP-9基因的定时控制表达，为提高临床基因治疗的效果提供了初步实验依据。     

A randomized prospective comparison of oral versus intraperitoneal ofloxacin as the primary treatment of CAPD peritonitis

Summary: Oral ofloxacin has been successfully used in our centres for the primary treatment of peritonitis complicating continous ambulatory peritoneal dialysis (CAPD). In view of the progressive rise in the resistance rate to ofloxacin among peritoneal bacterial isolates, a study was conducted to determine if oral ofloxacin remains a viable first line treatment for CAPD peritonitis in our centres and if the result can be improved by changing from an oral to an intraperitoneal (i.p.) route. In patients on three 2 L daily CAPD exchanges, ofloxacin given at the i.p. dosage of 200 mg loading followed by 25 mg/L of peritoneal dialysate achieved overnight trough peritoneal levels which are at least four times the minimal 90% inhibitory concentration (MIC90) of most bacterial pathogens without significant accumulation in the systemic circulation. This i.p. dosage was therefore chosen for the clinical study and the result was compared to that using ofloxacin given in the oral dosage of 400 mg loading followed by 300 mg once daily as maintenance. of all the recruited episodes, 35 were eligible for analysis. the overall primary cure rate including primary failures and relapses was 55.6% (10/18) in the oral treatment group and 70.6% (12/17) in the i.p. treatment group. the corresponding figures for gram positive bacterial (g +) infections were 36.4% and 50%, for gram negative bacterial (g -) infections were 66.7 and 80% and for culture negative infections were 75 and 80%. In culture positive cases, all treatment failures were due to resistant infections which were observed in 42.3% of all bacterial isolates, 47.1% of g + isolates and 33.3% of g - isolates. Due to the high background level of bacterial resistance among our CAPD population, ofloxacin monotherapy given either by the oral or the i.p. route can no longer be recommended for the primary treatment of CAPD peritonitis.     

CDE对干下型室间隔缺损、主动脉窦瘤的误诊原因分析

目的:分析彩色多普勒超声心动图(CDE)对先天性心脏病诊断的漏诊、误诊情况,提高确诊率。材料与方法:21例先天性心脏病患者,男11例,女10例,进行CDE与手术结果对照。结果:CDE对干下型室间隔缺损漏诊率为11％;对右窦瘤的误诊率为50％;对窦瘤破裂的误诊率为81％;对主动脉瓣关闭不全或脱垂的诊断符合率为100％。结论:超声探测时应仔细调整声束角度,多方了解合并畸形,明确分流信号,方可正确诊断。     

浅谈提高母子保健保偿投保率和巩固率

妇幼保健保偿的投保率和投保后的巩固率,可以从量的方面对保偿工作作出质的评价.为提高“两率”,应健全组织,广泛宣传,并借助于行政手段,提高广大群众投保的自觉性;要合理收缴并管好、用好保偿金,公平制订并坚决履行保偿合同,要搞好三级妇幼保健网的建设,强化管理,使群众对保偿制产生安全感和信任感.     

Prevalences of Diabetes and Hypertension and Their Relationship in Community Population of Jiangsu Province

Theprevalencesofnon--insulin--dependentdiabetesmellitus(NIDDM)andhypertensioninChinaaresteadilyincreasingalongwiththerapiddevelopmentofeconomy,theimprovementoflivingstandards,thepresenceofunhealthybehavioranddiet,andtheagingofthepopulationL'2J.Inthispaper,wecarriedoutalargepopulationsurveyinurbanandruralcommunitiesinJiangsuprovincefromOct.1996toJan.1997,inordertoestimatetheprevalencesofNIDDM,impairedglucosetolerance(IGT)andhypertensioninthecommunitypopulation,todemonstratetherelationsh…     

硝普钠与前列腺素E1控制性降压时对血清IL-6浓度的影响

目的探讨硝普钠(SNP)和前列腺素E1(PGE1)在行控制性降压时,对血清IL-6浓度的影响.方法 24例手术患者随机分为SNP组(S组)与PGE1组(P组),每组12例.均为全麻.控制性降压SNP自1蘥*kg-1*min-1开始,PGE1自0.1μg*kg-1*min-1开始,直至将平均动脉压降至50mm Hg,并维持1h.分别在麻醉前、降压前、降压1h、停降压1h后采血送检.结果停降压1h后,两组血清IL-6与降压前比较均升高,且差异均有显著性(S组P<0.05,P组P<0.01).两组间比较,P组较S组升高更加显著(P<0.05).结论 SNP与PGE1行控制性降压时,均可使血清IL-6增高.但IL-6升高是在停降压1h后,亦即手术开始2h后,同时SNP与PGE1组间比较有显著性差异.因此应该有选择地施行控制性降压及有选择地应用降压药物.     

Application of Electrical Impedance Principle in the Diagnosis of Diaphragm Fatigue

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