Effect of active warm-up on metabolism prior to and during intense dynamic exercise

PURPOSE: This study investigated whether active warm-up (AW) would increase muscle acetylcarnitine concentration before exercise, thereby reducing the reliance on nonoxidative ATP production during subsequent high-intensity exercise. METHODS: Six female subjects performed a 30-s sprint at 120% of their maximal power output on an electronically braked cycle ergometer 5 min after undertaking an active warm-up. To exclude any effect of muscle temperature (Tm) on metabolism, AW was compared with control (C), which involved passively heating the muscle to the same temperature as that achieved by active warm-up (37.1 +/- 0.3 vs 37.2 +/- 0.2 degrees C AW and C, respectively). RESULTS: Active warm-up significantly increased the concentration of acetylcarnitine from 4.5 +/- 1.5 mmol x kg(-1) dry muscle (dm)(-1) at rest to 9.4 +/- 1.6 mmol x kg dm(-1) before the onset of exercise. There was no change in acetylcarnitine concentration in C. During exercise the accumulation of muscle lactate was significantly less in AW compared with C (21.9 +/- 3.8 vs 34.3 +/- 2.3 mmol.kg dm, respectively). CONCLUSION: The main finding of this study was that there was less accumulation of blood and muscle lactate during intense dynamic exercise preceded by active warm-up, which could not be accounted for by a difference in T between trials immediately before the onset of exercise(m)     

Effects of reduced ambient temperature on fat utilization during submaximal exercise

PURPOSE: The influence of cold air exposure on fuel utilization during prolonged cycle exercise was investigated. METHODS: Nine male subjects cycled for 90 min in ambient temperatures of -10 degrees C, 0 degrees C, 10 degrees C, and 20 degrees C. External work performed between conditions was constant. Mean oxygen consumption (VO2) over the 90 min in the 20 degrees C trial corresponded to 64 +/- 5.8% VO2peak. RESULTS: Although mean skin temperature was different between trials (P < 0.05), rectal temperatures were not different. At -10 degrees C and 0 degrees C, the respiratory exchange ratio was higher compared with 10 degrees C and 20 degrees C (0.98 +/- 0.01 and 0.97 +/- 0.01 vs 0.92 +/- 0.01 and 0.91 +/- 0.01; P < 0.05). The associated rates of fat oxidation were lower at -10 degrees C and 0 degrees C compared with 10 degrees C and 20 degrees C (0.15 +/- 0.06 and 0.17 +/- 0.06 vs 0.35 +/- 0.06 and 0.40 +/- 0.04 g.min-1; P < 0.05). Blood glycerol was lower at -10 degrees C and 0 degrees C compared with 20 degrees C (P < 0.05); mean values were 0.13 +/- 0.0, 0.13 +/- 0.0, and 0.18 +/- 0.0 mmol.L-1 for the -10 degrees C, 0 degrees C, and 20 degrees C trials, respectively. Mean VO2 was lower in the -10 degrees C trial than the 20 degrees C trial (2.53 +/- 0.06 vs 2.77 +/- 0.09. L.min-1; P < 0.05). Mean blood glucose concentrations were lower at -10 degrees C than 20 degrees C (4.9 +/- 0.2 vs 5.3 +/- 0.1 mmol.L-1; P < 0.05). Although plasma epinephrine concentrations were greater during the 20 degrees C trial compared with all other trials (P < 0.05), plasma norepinephrine did not differ between trials. CONCLUSION: The diminished fat oxidation at colder temperatures potentially reflects a reduction in lipolysis and/or mobilization of FFA or impairment in the oxidative capacity of the muscle.     

Molecular analysis of PKU in Ireland

Classical phenylketonuria (PKU: McKusick No. 261600) is caused by mutations occurring at the phenylalanine hydroxylase (PAH) locus on chromosome 12 and has a prevalence in Ireland of 1 in 4500. We examined 304 independent alleles from 350 patients for the presence of six mutations and have characterized VNTR alleles within the minisatellite region 3' to the PAH gene in patients carrying the most prevalent mutation. R408W was the most common mutation found, with a relative frequency of 42%. All other mutations had relative frequencies of <10%. VNTR analysis showed that the R408W mutation is associated with the VNTR-8 allele in the Irish population, indicating that R408W is associated with RFLP haplotype 1. This differs from that reported from eastern Europe where R408W is associated with RFLP haplotype 2/VNTR-3; an observation which has led several groups to propose a Balto-Slavic origin for this mutation. These results support the hypothesis of a second, independent founding event for the R408W mutation on an RFLP haplotype 1 VNTR-8 chromsome background in the Irish/Celtic population.     

Spectrophotofluorometric and gas-liquid chromatographic methods for the estimation of mexiletine (Kö 1173) in plasma and urine

Methods are presented for the spectrophotofluorometric and gas-liquid chromatographic determination in plasma and urine of the anti-arrhythmic compound, mexiletine (Kö 1173). Both methods involve extraction of the drug from alkaline plasma with ether. In the spectrophotofluorometric method the compound is re-extracted into 0m?05n HCl and emission intensity determined at 300 nm with activation at 228 nm. In the chromatographic method the drug is acylated during evaporation of the ether. Both butyryl and acetyl derivatives could be used. Use of a nitrogen-sensitive detector increased the sensitivity and selectivity of the method and allowed more rapid analyses. There was good agreement between results obtained by spectrophotofluorometric and chromatographic methods.     

bla(SHV) Genes in Klebsiella pneumoniae: different allele distributions are associated with different promoters within individual isolates

Extended-spectrum β-lactamases (ESBLs) emerge by point mutation from non-extended-spectrum precursors. The aims of this study were to reveal the basis for variations in resistance levels found in a collection of 21 Klebsiella pneumoniae clinical isolates from Brisbane, Australia. Previous studies have shown that 20 of these isolates possess bla_SHV-11, bla_SHV-2a, and/or bla_SHV-12, and there is an association between the copy numbers of the ESBL-encoding genes and resistance levels. In this study, a real-time PCR method for interrogating the polymorphic sites at codons 238 and 240 was developed, and this confirmed the relationship between mutant gene copy numbers and resistance levels. The bla_SHV promoter region was cloned from one of the ESBL-expressing isolates, and this showed that bla_SHV genes exist downstream of two different promoters within this single isolate. These promoters have both been reported previously, and they differ by virtue of the presence or absence of an IS26 insertion. The bla_SHV copy numbers in cis with the different promoters were measured, and the copy number of the IS26 promoter was correlated with resistance levels. Cloning and analysis of PCR products showed that different bla_SHV variants existed in cis with individual promoters in individual isolates but that mutant genes were more abundant downstream of the IS26 promoter. There were no ESBL-positive isolates without this promoter. It was concluded that bla_SHV in cis with the IS26 promoter is located on an amplifiable replicon, and the presence of the IS26 insertion may facilitate the acquisition of an ESBL-positive phenotype.     

Dental Project Peru. Interview by Arveen Bajaj

For dentist Jacqui Nimmo, an action packed trip to Peru sowed the seed for much bigger things to come. From her experiences in the country, she founded the charity Dental Project Peru, as she tells Arveen Bajaj.     

An investigation into the accuracy of different types of thermometers

The traditional mercury-in-glass thermometer carries the potential risk of glass breakage and mercury spillage and health-care professionals have sought an alternative. This study examined the accuracy of three other types of temperature-measurement device--disposable, digital and tympanic--when compared with standard mercury thermometer readings.     

The use of ultrasound as an aid in the diagnosis of giant cell arteritis: a pilot study comparing histological features with ultrasound findings

AIMS: We present our preliminary experience with the use of ultrasound in aiding the diagnosis of giant cell arteritis (GCA). Schmidt et al have previously described a hypoechoic or "halo" effect surrounding the walls of affected arteries on examination with ultrasound. We illustrate these features and explore the attributes and limitations of this technique. METHOD: Two groups of patients were recruited: (1) patients with suspected GCA awaiting temporal artery biopsy and (2) patients with no history or symptoms of GCA of a similar age group. All the recruited patients underwent ultrasound examination of both temporal arteries. The findings on ultrasound were compared with the results of the histological specimens in group 1. For this study, the histological findings alone were used to define if a patient was suffering from GCA. No biopsies were taken in the patients in group 2. RESULTS: Out of 26 patients with suspected GCA, seven patients were found to be positive on biopsy, of which six had been identified on ultrasound. Six patients were found to be false positive on ultrasound, but all had moderate-to-severe features of arteriosclerosis on histology. A total of 13 patients were found to be negative on ultrasound and negative on biopsy for GCA, two of these patients had histological features of arteriosclerosis. In the group with no symptoms of GCA (12 patients), in two patients hypoechoic areas were detected. The results presented give a sensitivity of 86%, specificity of 68%, and positive predictive value of 50% for the use of ultrasound in the diagnosis of GCA. CONCLUSIONS: This preliminary study indicates that this test may be helpful in those patients with symptoms suggestive of GCA, but currently we cannot recommend any change of present practice.