氯地滴眼液对家兔眼压及房角组织影响的研究

目的 观察氯地滴眼液对家兔眼压及房角组织影响。方法 用６０只家兔设实验和对照组,以含０．１７５％氯霉素和０．１５％地塞米松的氯地跟液滴眼,每日４次,生理盐水对照。于１／２、１、２、３月测眼压后处死家兔以电镜观察房角组织变化。结果 眼压和房角组织结构与对照组无明显差异。结论 临床应用氯地眼液３月内是安全的。     

PRK治疗RK后残余近视散光二年疗效分析

目的： 评价准分子激光屈光性角膜切削术治疗放射状角膜切开术后残余近视散光的预测性、稳定性和安全性。方法： 对５２ 眼 Ｒ Ｋ 术后残余近视散光患者行再次 Ｐ Ｒ Ｋ 治疗, 并连续随访两年以上。术前残余球镜屈光度－ ３７５±１３２ Ｄ,残余柱镜屈光度－ ２３６±１２５ Ｄ, 最佳矫正视力均≥０８。结果： 术后 ６ 个月球镜屈光度－ ０３２±０２４ Ｄ, 柱镜屈光度－ ０７８±０５２ Ｄ, 裸眼视力≥１０ 者４６ 眼 （８８４６％ ）, 术后 ２４ 个月球镜屈光度－ ０３７±０４２ Ｄ, 柱镜屈光度－ ０５６±０３７ Ｄ, 裸眼视力≥１０ 者 ４２ 眼 （８０７６％ ）。最佳矫正视力下降 １ 行以上者 ３ 眼 （５７６％ ）, 实际球镜矫正度与预期矫正度相差１０ Ｄ 以内 ５０ 眼 （９０１５％ ）, 柱镜相差 １０ Ｄ以内５１ 眼 （９８０７％ ）。结论： Ｐ Ｒ Ｋ 治疗 Ｒ Ｋ 术后残余近视散光的两年随访结果表明, 该方法预测性强、稳定性好和安全性高, 特别在矫正残余散光方面, 其结果令人满意。     

左旋四氢巴马汀对单个豚鼠心室肌细胞钙电流的阻滞作用

AIM: To study the effect of l-tetrahydropalmatine (l-THP) on L-type calcium channel. METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2+ current in single cardiac myocyte. RESULTS: 1) l-THP 1, 10, and 100 micromol.L-1 reduced ICa-max from (999 +/- 93) pA to (700 +/- 111) pA, (582 +/- 66) pA, and (420 +/- 112) pA (n = 6, P < 0.01), respectively. 2) l-THP reduced the voltage at half-maximal inactivation (V1/2) of L-Ca2+ channel to more negative potentials by 9 mV (n = 5, P < 0.05). 3) l-THP caused both tonic and use-dependent reduction of Ca2+ current. Tonic block of l-THP on Ca2+ current was 46% +/- 8% (n = 6, P < 0.01). The degree of use dependent blocking was 13.5% +/- 2.4% (n = 6, P < 0.05) at 1 Hz, the degree increased to 44% +/- 5% (n = 6, P < 0.01) at 3 Hz. 4) l-THP delayed half-recovery time of Ca2+ channel recovery from inactivity from (94 +/- 39) ms to (170 +/- 42) ms(n = 6, P < 0.01). CONCLUSION: l-THP has a moderate inhibitory effect on L-Ca2+ current.     

平阳霉素联合地塞米松治疗颌面部大面积海绵状血管瘤

目的：观察平阳霉素（ＰＹＭ）联合地塞米松（ＤＸＭ）治疗颌面部大面积海绵状血管瘤的临床疗效和不良反应。方法：对９例临床确诊的颌面部范围６ｃｍ×７ｃｍ～１０ｃｍ×１２ｃｍ之间的大面积海绵状血管瘤,采用每周１次多点多方向瘤腔内注射药物,比较治疗前后患者的反应和肿瘤的形成变化。结果：全部病例肿瘤消失,无明显不良反应,经８个月～４６个月观察,临床治愈率为１００％。结论：ＰＹＭ联合ＤＸＭ能够治愈颌面部大面积海绵状血管瘤,具有完整保存组织正常形态和功能的特点,克服了单独使用ＰＹＭ带来的不良反应,提示两药在治疗上有协同作用。     

人肺癌相关抗原的基因克隆

目的克隆人肺癌相关抗原的基因。方法用λｇｔ１１Ｓｆｉ－Ｎｏｔ定向克隆载体构建人肺鳞癌细胞系Ｌ－７８的ｃＤＮＡ文库,并以抗人肺癌单抗ＡＬＴ－０４为探针进行了筛选。对名为ｈｌｃ－１４的ｃＤ－ＮＡ克隆作了测序分析与进一步研究。用重组的ｐＸＪ４１－ｈｌｃ１４质粒转染ＮＩＨ３Ｔ３细胞,通过免疫组化法对克隆基因进行了表达检测与鉴定,还观察了转染细胞的软琼脂集落形成能力。结果建成１．４×１０６ｐｆｕ／ｍｌ人肺癌ｃＤＮＡ文库,经过四轮免疫学筛选获得６个ｃＤＮＡ克隆,最大的ｃＤＮＡ克隆为ｈｌｃ－１４,长９５４ｂｐ。对该克隆的测序结果与基因库资料比较,未发现同源序列。ｈｌｃ－１４ｃＤＮＡ转染的ＮＩＨ３Ｔ３细胞有明显的肺癌相关抗原表达,并且在软琼脂中的集落形成能力增强。结论ｈｌｃ－１４是一个新的肺癌相关抗原的ｃＤＮＡ序列。这一序列的发现为阐明肺癌的发病机理、探索肺癌基因治疗的靶基因提供了新的线索     

Interleukln-8 stimulates angiogenesis in rats

To test the hypothesis that the cytokines interleukin-6 (IL-6) and IL-8 may play regulatory roles in the aberrant neovascularization in chronic inflammatory diseases, we examined their effects in a rat sponge model and compared their actions with those of IL-1 and lumor necrosis factor- (TNF-). Daily doses of 3 pmol IL-8, IL-1, TNF-, but nol IL-6, significantly accelerated the sponge-induced angio-genesis. Although lower doses (0.3 pmol) of these cytokines were inactive, IL-1 acted synergistically with subthreshold daily doses (10 pmol) of substance P (SP) and bradykinin (BK) to produce an intense angiogenic response. In contrast, IL-8 only interacted positively with IL-1, but not TNF-, SP, or BK. There was no synergism or antagonism between IL-6 and SP. These results demonstrate the discrete interactions between angiogenic factors and cytokines in chronic inflammation and suggest that the sponge model is a good means for the study of such interactions.     

Chemosensitization of murine fibrosarcoma cells to drugs affected by the multidrug resistance phenotype by the antidepressant trazodone - an experimental-model for the reversal of intrinsic drug-resistance

A variety of resistance phenotypes to cytotoxic agents in bacteria, protozoa parasites and mammalian cells are mediated by evolutionarily conserved proteins of the mdr family. The finding that chloroquine resistance in the malarial parasite, Plasmodium falciparum, that is mediated by an mdr-1 gene product can be circumvented by tricyclic antidepressant drugs has stimulated the present study to assess whether this class of agents might also modulate the multidrug resistance (MDR) phenotype(s) in mammalian tumor cells. The possible chemosensitizing effects of nine antidepressant drugs have been tested against the UV-2237M murine fibrosarcoma line and its MDR variant. At nontoxic concentrations all nine antidepressants markedly enhanced the cytotoxicity of ADR against the parental cells but were much less effective against the MDR cells. The most active antidepressant, trazodone, also enhanced the cytotoxicities of vinblastine and vincristine, but not those of actinomycin D, mitomycin C, or 5-fluorouracil. The parental cells treated with trazodone exhibited an increased accumulation of intracellular ADR, but lacked detectable alterations in the expression and drug-binding activity of plasma membrane P-glycoprotein, and trazodone did not affect the activities of isolated protein kinase C and calmodulin. These data suggest that the antidepressant drug trazodone may be useful in the reversal of the intrinsic drug resistance of tumor cells that express low levels of P-glycoprotein.     

30例糖尿病合并冠心病患者的心率变异性分析

目的：研究2型糖尿病（DM）合并冠心病（CHD）与单纯2型DM对心率变异性（HRV）的影响，方法：对66例2型DM患者，其中30例合并CHD，36例为单纯2型DM，进行24h HEV时域及频域分析，同时与年龄，性别相当的20例健康成年人HRV资料比较。结果：（1）2型DM患者各时域及频域分析指标均较正常组降低（P＜0．05或P＜0．01），但VLF和LF／HF增高（P＜0．05或P＜0．01），（2）与单纯2型DM患者比较，有CHD合并症的2型DM患者，HRV各参数也明显降低（P＜0．05，P＜0．01），但VLF和LF／HF增高（P＜0．05），结论：2型DM患者存在着HRV异常，其异常程度与病变轻重成正比，合并CHD者异常更显著，提示2型DM患者的HRV分析有助于预测恶性心血管疾病的发生。     

生孢梭菌孢子作为生物指示剂用于残存概率灭菌工艺验证的可行性

测定并分析生孢梭菌孢子在不同介质及不同温度下的耐热参数，评价生孢梭菌孢子的耐热特性及其作为生物指示剂在残存概率湿热灭菌工艺验证中的应用。     

双苓扶正抗癌胶囊对小鼠肝癌的影响

目的：研究双苓扶正抗癌胶囊对小鼠肝癌的抑制作用及作用机制。方法：建立小鼠肝癌（Heps）实体瘤及腹水癌模型，观察抑瘤率和生长延长率，并用流式细胞术（FCM）检测小鼠肝癌实体瘤的细胞周期及癌细胞凋亡率。结果：双苓扶正抗癌胶囊在15g和30g/kg剂量下，对小鼠肝癌实体瘤有显著抑制作用，也能延长荷腹水型肝癌小鼠的生存期；还可引起小鼠肝癌瘤细胞S期及G2-M期细胞减少，G0-G1期细胞及凋亡细胞增加。结论：双苓扶正抗癌胶囊对小鼠移植性肝癌具有抑制作用，其作用机制可能与抑制细胞周期中S期DNA合成、干扰细胞的分裂增殖速度及诱导癌细胞凋亡有关。