全文获取类型
收费全文 | 163篇 |
免费 | 5篇 |
国内免费 | 4篇 |
专业分类
儿科学 | 6篇 |
妇产科学 | 4篇 |
基础医学 | 12篇 |
口腔科学 | 3篇 |
临床医学 | 13篇 |
内科学 | 54篇 |
皮肤病学 | 2篇 |
特种医学 | 51篇 |
外科学 | 2篇 |
综合类 | 3篇 |
预防医学 | 3篇 |
眼科学 | 1篇 |
药学 | 8篇 |
中国医学 | 7篇 |
肿瘤学 | 3篇 |
出版年
2023年 | 1篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2017年 | 1篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 1篇 |
2012年 | 5篇 |
2011年 | 1篇 |
2010年 | 4篇 |
2009年 | 4篇 |
2008年 | 3篇 |
2007年 | 8篇 |
2006年 | 3篇 |
2005年 | 1篇 |
2004年 | 1篇 |
2003年 | 1篇 |
2001年 | 1篇 |
1999年 | 3篇 |
1998年 | 7篇 |
1997年 | 12篇 |
1996年 | 10篇 |
1995年 | 10篇 |
1994年 | 19篇 |
1993年 | 4篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 9篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 8篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 7篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有172条查询结果,搜索用时 15 毫秒
51.
Grzegorzewski KJ; Komschlies KL; Franco JL; Ruscetti FW; Keller JR; Wiltrout RH 《Blood》1996,88(11):4139-4148
Administration of recombinant human interleukin-7 (rhIL-7) to mice increases the exportation of myeloid progenitors (colony-forming unit [CFU]-c and CFU-granulocyte erythroid megakaryocyte macrophage [CFU- GEMM]) from the bone marrow (BM) to peripheral organs, including blood, and also increases the number of primitive progenitor and stem cells in the peripheral blood (PB). We now report that combined treatment of mice with rhIL-7 and recombinant human granulocyte-colony stimulating factor (rhG-CSF) stimulates a twofold to 10-fold increase in the total number of PB CFU-c, and a twofold to fivefold increase in the total number of PB CFU-spleen at day 8 (CFU-S8) over the increase stimulated by rhIL-7 or rhG-CSF alone. In addition, the quality of mobilized cells with trilineage, long-term marrow-repopulating activity is maintained or increased in mice treated with rhIL-7 and rhG-CSF compared with rhIL- 7 or rhG-CSF alone. These differences in mobilizing efficiency suggest qualitative differences in the mechanisms by which rhIL-7 and rhG-CSF mobilize progenitor cells, in fact, the functional status of progenitor cells mobilized by rhIL-7 differs from that of cells mobilized by rhG- CSF in that the incidence of actively cycling (S-phase) progenitors obtained from the PB is about 20-fold higher for rhIL-7-treated mice than for mice treated with rhG-CSF. These results suggest the use of rhIL-7-mobilized progenitor/stem cells for gene-modification and tracking studies, and highlight different functions and rates of repopulation after reconstitution with PB leukocytes obtained from mice treated with rhIL-7 versus rhG-CSF. 相似文献
52.
Muregi FW Ishih A Miyase T Suzuki T Kino H Amano T Mkoji GM Terada M 《Journal of ethnopharmacology》2007,111(1):190-195
Methanolic extracts from 15 medicinal plants representing 11 families, used traditionally for malaria treatment in Kenya were screened for their in vivo antimalarial activity in mice against a chloroquine (CQ)-tolerant Plasmodium berghei NK65, either alone or in combination with CQ. The plant parts used ranged from leaves (L), stem bark (SB), root bark (RB), seeds (S) and whole plant (W). When used alone, extracts from seven plants, Clerodendrum myricoides (RB), Ficus sur (L/SB/RB), Maytenus acuminata (L/RB), Rhamnus prinoides (L/RB), Rhamnus staddo (RB), Toddalia asiatica (RB) and Vernonia lasiopus (RB) had statistically significant parasitaemia suppressions of 31.7-59.3%. In combination with CQ, methanolic extracts of Albizia gummifera (SB), Ficus sur (RB), Rhamnus prinoides and Rhamnus staddo (L/RB), Caesalpinia volkensii (L), Maytenus senegalensis (L/RB), Withania somnifera (RB), Ekebergia capensis (L/SB), Toddalia asiatica (L/RB) and Vernonia lasiopus (L/SB/RB) gave statistically significant and improved suppressions which ranged from 45.5 to 85.1%. The fact that these activities were up to five-fold higher than that of extract alone may suggest synergistic interactions. Remarkable parasitaemia suppression by the extracts, either alone or in combination with CQ mostly resulted into longer mouse survival relative to the controls, in some cases by a further 2 weeks. Plants, which showed significant antimalarial activity including Vernonia lasiopus, Toddalia asiatica, Ficus sur, Rhamnus prinoides and Rhamnus staddo warrant further evaluation in the search for novel antimalarial agents against drug-resistant malaria. 相似文献
53.
54.
Muregi FW 《Current drug discovery technologies》2010,7(4):280-316
Efforts to develop an effective malarial vaccine are yet to be successful and thus chemotherapy remains the mainstay of malaria control strategy. Unfortunately, Plasmodium falciparum, the parasite that causes about 90% of all global malaria cases is increasingly becoming resistant to classical antimalarials, necessitating a search for new chemotherapeutics preferably with novel modes of action. Today, rational drug discovery strategy is gaining new impetus as knowledge of malaria parasite biology expands, aided by the parasite genome database and improved bioinformatics tools. Drug development is a laborious, time consuming and costly process, and thus the "useful therapeutic lives" (UTLs) of new drugs should be commensurate with the resources invested in their development. Historical evidence on development and evolution of resistance to classical antimalarial drugs shows that the mode of action of a drug influences its UTL. Drugs that target single and specific targets such as antimalarial antifolates and atovaquone (ATQ) are rendered ineffective within a short time of their clinical use, unlike drugs with pleiotropic action such as chloroquine (CQ) and artemisinins (ART) with long UTLs. Unfortunately, almost all new targets currently being explored for development of novel drugs belong to the "specific target" other than the "multiple target" category, and is plausible that such drugs will have short UTLs. This review relates the pleiotropic action of CQ and ART with their long UTLs, and discusses their relevance in rational drug development strategies. Novel targets with potential to yield drugs with long UTLs are also explored. 相似文献
55.
V Wewer KM Christiansen LP Andersen FW Henriksen JP Hart Hansen M Tvede PA Krasilnikoff 《Acta paediatrica (Oslo, Norway : 1992)》1994,83(12):1276-1281
Helicobacter pylori was cultured and Helicobacter-like organisms (HLO) were seen in 6 (16%) of 37 children with recurrent abdominal pain. Five children had concomitant histological inflammation, but none had endoscopic changes. All 6 children demonstrated positive serology. Compared with the total group, they were more often from developing countries, larger families and lower Social groups. Treatment with phenoxymethyl penicillin and colloidal bismuth subcitrate did not result in side effects or elevated serum levels of serum bismuth. Three children demonstrated metronidazole-resistant strains and the treatment of these children remained an unsolved problem. Among the 31 H. pylori/ HLO negative children 8 (26%) demonstrated histological changes, 5 (16%) endoscopic changes and 11 (35%) had positive serology. In conclusion, pathological findings at upper gastrointestinal endoscopy are common in children with recurrent abdominal pain. Because of disconcordance between endoscopy, histology and culture, we recommend that biopsies should always be taken to clarify the diagnosis. 相似文献
56.
Neuer A; Lam KN; Tiller FW; Kiesel L; Witkin SS 《Human reproduction (Oxford, England)》1997,12(5):925-929
Recent evidence suggests that Chlamydia trachomatis can persist in the
female upper genital tract in an unculturable state. Since unsuspected C.
trachomatis infection has been associated with adverse in-vitro
fertilization (IVF) outcome we sought to detect further evidence of C.
trachomatis in the genital tracts of women undergoing IVF. The prevalence
and distribution of antibodies to the major structural proteins of C.
trachomatis in paired follicular fluid and sera of women undergoing IVF
were examined. Sera and follicular fluid samples from 149 women were
assayed for immunoglobulin (Ig)G and IgA antibodies to two C. trachomatis
antigens, the major outer membrane protein (MOMP) and a recombinant
lipopolysaccharide (rLPS) fragment. Additionally, the expression of human
60 kDa heat shock protein (hsp 60) in follicular fluid was determined. All
cervical and follicular fluid samples were negative for C. trachomatis by
polymerase chain reaction, ligase chain reaction and DNA probe. Sera from
60% of the subjects were positive for antichlamydial rLPS IgG; 36% were
positive for anti-MOMP IgG. Similarly, rLPS-directed and MOMP-directed IgA
were detected in sera of 34 and 14% of the subjects respectively. IgG
antibodies to MOMP and rLPS were detected in 42 and 41% of the follicular
fluid examined respectively. Anti-MOMP IgA was identified in 8.7% of the
follicular fluid while 27.5% were positive for anti-rLPS IgA. Human hsp 60
expression was documented in 11.6% of the follicular fluid tested. IgA
antibodies to both MOMP (P = 0.03) and rLPS (P = 0.02) in follicular fluid
were associated with a failure to become pregnant after embryo transfer.
IgG antibodies in sera and follicular fluid and IgA antibodies in sera were
unrelated to IVF outcome. Similarly only anti- MOMP IgA (P = 0.02) and
anti-rLPS IgA (P = 0.04) in follicular fluid were correlated with human hsp
60 expression in follicular fluid. The unique association between IgA
antibodies to two chlamydial antigens in follicular fluid and both hsp 60
expression and IVF failure provides further support for the possibility
that a persistent upper genital tract chlamydial infection contributes to
IVF failure in some women.
相似文献
57.
Cyclic adenosine monophosphate response to prostaglandin E2 on subpopulations of human lymphocytes
下载免费PDF全文
![点击此处可从《The Journal of experimental medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M Kasai JC Leclerc L McVay-Boudreau FW Shen H Cantor 《The Journal of experimental medicine》1979,150(5):1260-1264
Receptors for prostaglandin E2 or histamine were measured on subpopulations of human lymphocytes, using the cyclic AMP increase after exposure to prostaglandin or histamine as an indicator for the presence of receptors. The cyclic AMP response to prostaglandin E2 was similar in unfractionated lymphocytes and the T-enriched and T-depleted fractions. Within the T-enriched population, T cells bearing a receptor for the Fc portion of IgG (T gamma-cells) had a 27.4-fold rise in cyclic AMP after exposure to prostaglandin E2, whereas the remaining T cells (non-T gamma cells) had a fourfold increase. It would appear that prostaglandin receptors are concentrated on a small subfraction of T gamma cells, comprising approximately 15% of the T-cell population. The cyclic AMP response to histamine was less than twofold in all lymphocyte fractions. 相似文献
58.
59.
Water distribution in blood during sickling of erythrocytes 总被引:4,自引:0,他引:4
Plasma urea and protein determinations proved suitable for measuring changes in total diffusible water and plasma volume in whole blood. Deoxygenation by saturation with carbon dioxide at 25 degrees C caused no change in plasma urea, but a significant increase in plasma protein concentration was induced with both normal and sickle-cell (HbSS) blood. Thus in HbSS blood there was no binding or trapping of water as a result of sickling and there was a normal influx of water into the cells (Bohr effect) despite the polymerization of the hemoglobin molecules with sickling. Consistent with this observation was the finding that the deoxygenation induced a similar increase in concentration of the plasma cations, sodium plus potassium. HbSS erythrocytes neither lost nor gained water under the more physiologic conditions of deoxygenation with a 95% nitrogen, 5% carbon dioxide gas mixture. 相似文献
60.