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991.
Hsp27 knockdown using nucleotide-based therapies inhibit tumor growth and enhance chemotherapy in human bladder cancer cells 总被引:13,自引:0,他引:13
Kamada M So A Muramaki M Rocchi P Beraldi E Gleave M 《Molecular cancer therapeutics》2007,6(1):299-308
Heat shock protein 27 (Hsp27) is a cytoprotective chaperone that is phosphoactivated during cell stress that prevents aggregation and/or regulate activity and degradation of certain client proteins. Recent evidence suggests that Hsp27 may be involved in tumor progression and the development of treatment resistance in various tumors, including bladder cancer. The purpose of this study was to examine, both in vitro and in vivo, the effects of overexpression of Hsp27 and, correspondingly, the down-regulation of Hsp27 using small interfering (si) RNA and OGX-427, a second-generation antisense oligonucleotide targeting Hsp27. Hsp27 overexpression increased UMUC-3 cell growth and resistance to paclitaxel. Both OGX-427 and Hsp27 siRNA decreased Hsp27 protein and mRNA levels by >90% in a dose- and sequence-specific manner in human bladder cancer UMUC-3 cells. OGX-427 or Hsp27 siRNA treatment induced apoptosis and enhanced sensitivity to paclitaxel in UMUC-3 cells. In vivo, OGX-427 significantly inhibited tumor growth in mice, enhanced sensitivity to paclitaxel, and induced significantly higher levels of apoptosis compared with xenografts treated with control oligonucleotides. Collectively, these findings suggest that Hsp27 knockdown with OGX-427 and combined therapy with paclitaxel could be a novel strategy to inhibit the progression of bladder cancer. 相似文献
992.
Sawayama Hiroshi Miyamoto Yuji Mima Kosuke Kato Rikako Ogawa Katsuhiro Hiyoshi Yukiharu Shimokawa Mototsugu Akiyama Takahiko Kiyozumi Yuki Iwagami Shiro Iwatsuki Masaaki Baba Yoshifumi Yoshida Naoya Baba Hideo 《International journal of clinical oncology / Japan Society of Clinical Oncology》2021,26(11):2037-2045
International Journal of Clinical Oncology - Iron deficiency anemia is represented in colorectal cancer (CRC) patients. Iron surplus load to increase non-transferrin bound iron (NTBI), and NTBI... 相似文献
993.
Uemura Motohide Nakaigawa Noboru Sassa Naoto Tatsugami Katsunori Harada Kenichi Yamasaki Toshinari Matsubara Nobuaki Yoshimoto Takuya Nakagawa Yuki Fukuyama Tamaki Oya Mototsugu Shinohara Nobuo Uemura Hirotsugu Tsuzuki Toyonori 《International journal of clinical oncology / Japan Society of Clinical Oncology》2021,26(11):2085-2086
International Journal of Clinical Oncology - 相似文献
994.
995.
Y Sekine Y Takai O Nishii N Kudaka A Onozawa H Arai J Kizu Y Arakawa O Tsutsumi 《Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan》2001,121(12):995-1004
In the Department of Obstetrics and Gynecology at our hospital, a team of doctors, pharmacists, nurses, and other medical staff was established to prepare a clinical pathway for laparoscopic cystectomy. Various data on clinical charts including the use of drugs were collected from 57 patients by pharmacists and nurses. Based on the analysis of these data, hospitalization period, method of preoperative bowel preparation, time to initiation of food intake, duration of antibiotic administration, and time and content of pharmaceutical instructions to patients of dosage and administration were determined. Criteria for variances requiring the doctor's directions were determined for fever, wound pain, and vomiting. The clinical pathway established here allows of not only the efficient and uniform care of patients, but also the active exchange of opinions among members of the medical team. Moreover, most patients who replied to a questionnaire said that they were at ease during hospitalization because they had received detailed information about the clinical pathway including the use of drugs before surgery. Thus, the participation of pharmacists on a medical team that is introducing a clinical pathway is particularly important because the use of drugs and pharmaceutical care are an important part of good patient care. 相似文献
996.
997.
Gouji Toyokawa Kazuki Takada Tatsuro Okamoto Mototsugu Shimokawa Yuka Kozuma Taichi Matsubara Naoki Haratake Shinkichi Takamori Takaki Akamine Masakazu Katsura Fumihiro Shoji Yoshinao Oda Yoshihiko Maehara 《Clinical lung cancer》2017,18(6):e375-e383
Introduction
The development of immune checkpoint inhibitors against programmed death 1 has paved the way for a new era of treatment of lung cancer. Programmed death-ligand 1 (PD-L1) is expected to predict the response of immune checkpoint inhibitors in lung cancer. Predicting PD-L1 expression using a noninvasive method before immunotherapy would, therefore, help identify patients for whom immunotherapy can be successful.Patients and Methods
A total of 394 patients with resected lung adenocarcinoma who had undergone preoperative thin-section computed tomography (CT) were analyzed for PD-L1 expression by immunohistochemistry and evaluated to determine the association between PD-L1 expression and CT characteristics, including convergence, surrounding ground glass opacity (GGO), air bronchogram, notching, pleural indentation, spiculation, and cavitation.Results
Of the 394 patients, 78 (19.8%) were positive and 316 (80.2%) were negative for PD-L1 expression. Univariate analysis demonstrated that PD-L1+ adenocarcinoma was significantly associated with the presence of convergence (P < .01), notching (P < .01), spiculation (P < .01), and cavitation (P < .01) and the absence of surrounding GGO (P < .01) compared with PD-L1? cases. On multivariate analysis, the presence of convergence (P < .01) and cavitation (P < .01) and the absence of surrounding GGO (P = .02) and air bronchogram (P = .03) were significantly associated with PD-L1 expression.Conclusion
PD-L1+ adenocarcinoma cases showed convergence and cavitation more frequently than did PD-L1? cases. In contrast, surrounding GGO and air bronchogram were observed less frequently in PD-L1+ cases than in PD-L1? cases. These results will prove helpful in identifying PD-L1–expressing adenocarcinoma by CT before immunotherapy. 相似文献998.
Induction of HLA‐B*40:02‐restricted T cells possessing cytotoxic and suppressive functions against haematopoietic progenitor cells from a patient with severe aplastic anaemia
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Yoshiki Akatsuka Kohei Hosokawa Hiroyuki Maruyama Akinao Okamoto Takamasa Katagiri Keiko Shiraishi Yuko Murayama Sachiko Tsuzuki‐Iba Yuuki Mizutani Chikako Nishii Naoki Yamamoto Ayako Demachi‐Okamura Kiyotaka Kuzushima Seishi Ogawa Nobuhiko Emi Shinji Nakao 《British journal of haematology》2016,172(1):131-134
999.
Nagaaki Kotera Mototsugu Tanaka Mari Aoe Masatomo Chikamori Tomoko Honda Ayako Ikenouchi Rika Miura Mai Sugahara Satoshi Furuse Katsunori Saito Naobumi Mise 《Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy》2016,20(6):655-660
Peritonitis remains an important cause of morbidity and mortality in peritoneal dialysis (PD) patients, but its incidence and the distribution of causative organisms vary widely between institutions and age groups. This study was performed to investigate the recent status and risk factors of PD‐related peritonitis and to clarify differences between age groups. We retrospectively reviewed the medical records of 119 PD patients treated at our department between January 2002 and January 2013. We calculated both overall and organism‐specific peritonitis rates and also analyzed risk factors. Sixty‐three episodes of peritonitis occurred during 261.5 patient‐years for an incident rate of 0.24 episodes/patient‐year. Multivariate analysis showed that older age (≥65 years) and hypoalbuminemia (<3.0 g/dL) were associated with an increased risk of peritonitis (P = 0.035 and P = 0.029, respectively). In elderly patients (≥65 years old), the rate of peritonitis due to Gram‐positive and Gram‐negative bacteria was 0.17 and 0.08 episodes/patient‐year, respectively, and Gram‐positive peritonitis was markedly more frequent than in younger patients (<65 years old). In particular, there was a high frequency of Staphylococcus aureus peritonitis in elderly patients (0.09 episodes/patient‐year) and it had a poor outcome. At our department, the risk of peritonitis was increased in older patients and patients with hypoalbuminemia. The distribution of causative organisms was markedly different between age groups and analysis of organism‐specific peritonitis rates helped to identify current problems with our PD program. 相似文献
1000.
Prognostic value of EGFR mutations in surgically resected pathological stage I lung adenocarcinoma
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