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71.
Montes de Jesus F. M. Kwee T. C. Kahle X. U. Nijland M. van Meerten T. Huls G. Dierckx R. A. J. O. Rosati S. Diepstra A. van der Bij W. Verschuuren E. A. M. Glaudemans A. W. J. M. Noordzij W. 《European journal of nuclear medicine and molecular imaging》2020,47(3):529-536
European Journal of Nuclear Medicine and Molecular Imaging - Post-transplant lymphoproliferative disorder (PTLD) is a serious complication after solid organ and hematopoietic stem cell... 相似文献
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Daniel Ortuño-Sahagún Raúl Montes González Ester Verdaguer Verónica Chaparro Huerta Blanca M. Torres-Mendoza Lourdes Lemus Martha Catalina Rivera-Cervantes A. Camins C. Beas Zárate 《Journal of molecular neuroscience : MN》2014,52(3):366-377
Current knowledge concerning the molecular mechanisms of the cellular response to excitotoxic insults in neurodegenerative diseases is insufficient. Although glutamate (Glu) has been widely studied as the main excitatory neurotransmitter and principal excitotoxic agent, the neuroprotective response enacted by neurons is not yet completely understood. Some of the molecular participants have been revealed, but the signaling pathways involved in this protective response are just beginning to be identified. Here, we demonstrate in vivo that, in response to the cell damage and death induced by Glu excitotoxicity, neurons orchestrate a survival response through the extracellular signal-regulated kinase (ERK) signaling pathway by increasing ERK expression in the rat hippocampal (CA1) region, allowing increased neuronal survival. In addition, this protective response is specifically reversed by U0126, an ERK inhibitor, which promotes cell death only when it is administered together with Glu. Our findings demonstrate that the ERK signaling pathway has a neuroprotective role in the response to Glu-induced excitotoxicity in hippocampal neurons. Therefore, the ERK signaling pathway may be activated as a cellular response to excitotoxic injury to prevent damage and neural loss, representing a novel therapeutic target in the treatment of neurodegenerative diseases. 相似文献
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Ricardo A. Chvez Montes Anna Haber Jeremy Pardo Robyn F. Powell Upendra K. Divisetty Anderson T. Silva Tania Hernndez-Hernndez Vanildo Silveira Haibao Tang Eric Lyons Luis Rafael Herrera Estrella Robert VanBuren Melvin J. Oliver 《Proceedings of the National Academy of Sciences of the United States of America》2022,119(5)
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Neutrophils extracellular traps damage Naegleria fowleri trophozoites opsonized with human IgG 总被引:1,自引:0,他引:1
A. Contis‐Montes de Oca M. Carrasco‐Yépez R. Campos‐Rodríguez J. Pacheco‐Yépez P. Bonilla‐Lemus J. Pérez‐López S. Rojas‐Hernández 《Parasite immunology》2016,38(8):481-495
Naegleria fowleri infects humans through the nasal mucosa causing a disease in the central nervous system known as primary amoebic meningoencephalitis (PAM). Polymorphonuclear cells (PMNs) play a critical role in the early phase of N. fowleri infection. Recently, a new biological defence mechanism called neutrophil extracellular traps (NETs) has been attracting attention. NETs are composed of nuclear DNA combined with histones and antibacterial proteins, and these structures are released from the cell to direct its antimicrobial attack. In this work, we evaluate the capacity of N. fowleri to induce the liberation of NETs by human PMN cells. Neutrophils were cocultured with unopsonized or IgG‐opsonized N. fowleri trophozoites. DNA, histone, myeloperoxidase (MPO) and neutrophil elastase (NE) were stained, and the formation of NETs was evaluated by confocal microscopy and by quantifying the levels of extracellular DNA. Our results showed N. fowleri induce the liberation of NETs including release of MPO and NE by human PMN cells as exposure interaction time is increased, but N. fowleri trophozoites evaded killing. However, when trophozoites were opsonized, they were susceptible to the neutrophils activity. Therefore, our study suggests that antibody‐mediated PMNs activation through NET formation may be crucial for antimicrobial responses against N. fowleri. 相似文献
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Paloma Merino Jesús Guinea Irene Muñoz-Gallego Patricia González-Donapetry Juan Carlos Galán Nerea Antona Gustavo Cilla Silvia Hernáez-Crespo José Luis Díaz-de Tuesta Ana Gual-de Torrella Fernando González-Romo Pilar Escribano Miguel Ángel Sánchez-Castellano Mercedes Sota-Busselo Alberto Delgado-Iribarren Julio García Rafael Cantón Patricia Muñoz Mila Montes 《Clinical microbiology and infection》2021,27(5):758-761
ObjectivesThe standard RT-PCR assay for coronavirus disease 2019 (COVID-19) is laborious and time-consuming, limiting testing availability. Rapid antigen-detection tests are faster and less expensive; however, the reliability of these tests must be validated before they can be used widely. The objective of this study was to determine the performance of the Panbio? COVID-19 Ag Rapid Test Device (PanbioRT) (Abbott) in detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swab specimens.MethodsThis prospective multicentre study was carried out in ten Spanish university hospitals and included individuals with clinical symptoms or epidemiological criteria of COVID-19. Only individuals with ≤7 days from the onset of symptoms or from exposure to a confirmed case of COVID-19 were included. Two nasopharyngeal samples were taken to perform the PanbioRT as a point-of-care test and a diagnostic RT-PCR test.ResultsAmong the 958 patients studied, 325 (90.5%) had true-positive results. The overall sensitivity and specificity for the PanbioRT were 90.5% (95%CI 87.5–93.6) and 98.8% (95%CI 98–99.7), respectively. Sensitivity in participants who had a threshold cycle (CT) < 25 for the RT-PCR test was 99.5% (95%CI 98.4–100), and in participants with ≤5 days of the clinical course it was 91.8% (95%CI 88.8–94.8). Agreement between techniques was 95.7% (κ score 0.90; 95%CI 0.88–0.93).ConclusionsThe PanbioRT performs well clinically, with even more reliable results for patients with a shorter clinical course of the disease or a higher viral load. The results must be interpreted based on the local epidemiological context. 相似文献
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