胰岛素样生长因子-Ⅰ对软骨细胞凋亡的抑制

目的:探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对软骨细胞增殖及白细胞介素-1(IL-1)诱导软骨细胞凋亡的影响,揭示其抗损伤作用机制,为关节软骨损伤治疗提供理论依据。方法:分离培养人胚胎关节软骨细胞,采用四氮甲基唑蓝(MTT)法测定不同含量软骨细胞增殖活性的变化,利用光镜、电镜、DNA电泳及流式细胞仪测定作为凋亡检测指标。结果:IGF-Ⅰ呈剂量依赖式促软骨细胞增殖,当IGF-Ⅰ含量达50μg/L时,促软骨细胞增殖作用达最大值。IL-1组光镜、电镜下可见典型的细胞凋亡形态学改变,琼脂糖凝胶电泳示特征性的DNA梯状条带,IGF-Ⅰ处理组未见明显凋亡征象。流式细胞仪检测发现,IGF-Ⅰ处理后软骨细胞凋亡率显著降低。结论:IGF-Ⅰ能促进软骨细胞增殖,对IL-1诱导的软骨细胞凋亡具有保护作用。     

李振华教授调理脾胃用药规律探讨

李振华教授，生于1924年，河南省洛宁县人。著名中医脾胃学专家，全国片批名老中医。国务院特殊津贴专家，原中华中医药学会常务理事、第七届全国人大代表，曾任河南中医学院院长，从事中医临床与教学六十余载，长于内科杂病，晚年潜心于脾胃学说研究，著有《中国传统脾胃病学》等，并负责研究河南省重点科研项目“脾胃气虚本质的研究”及“七五”国家重点科技攻关项目“慢性萎缩性胃炎脾虚证临床及实验研究”，且均荣获河南省科技进步成果奖。经过多年临床实践，李老整理总结出调理脾胃的用药规律。提出通过药物件味归经，以及药效应用与脾胃的生理、病理特点相结合的川药大法。     

Prognostic role of alveolar-arterial oxygen pressure difference in acute pulmonary embolism.

BACKGROUND: This study investigated the utility of the alveolar - arterial oxygen pressure difference (AaDO (2)) in predicting the short-term prognosis of acute pulmonary embolism (PE). METHODS AND RESULTS: This study retrospectively enrolled 114 consecutive patients with acute PE, diagnosed by either spiral computed tomography or high probability ventilation - perfusion lung scans. During the first 24 h of admission, all patients had initial artery blood gas collected under room air. Patient exclusion criteria were chronic lung disease, septic emboli, and moderate and low probability lung scans. Patients were assigned to 2 groups based on either 30-day death or a 30-day composite event. Receiver operating characteristic analyses was used to determine the AaDO(2) cut-off value for predicting primary and composite endpoints. Statistical analysis demonstrated significant differences in AaDO(2) between the 30-day composite endpoint group and the 30-day composite event-free survival group (p=0.012). The AaDO(2) had a strong trend between the 30-day death group and the survival group (p=0.062). The best cut-off value for AaDO(2) was 53 mmHg and using this, the positive predictive value for 30-day death was 25% and the negative predictive value was 92%. For the 30-day composite endpoint, the positive predictive value for AaDO(2) was 35%, and the negative predictive value was 84%. In this study, thrombocytopenia was also an indicator of poor prognosis for patients with acute PE. CONCLUSION: The AaDO(2) measurement is a highly useful and simple measurement for predicting short-term prognosis in patients with acute PE. It has high negative predictive value and moderate positive predictive value for 30-day death and 30-day composite event. Aggressive thrombolytic treatment strategies should be considered for patients with an initial poor prognostic parameter (ie, AaDO(2) >or=53 mmHg).     

医德为先 防治结合——记儿科名医王仆诚

王仆诚（1877-1961），原名联福。早年在药店学习医药。1903年起开业行医，擅治小儿疾病，驰誉成都，人称之为“王小儿”。1953年，四川省行署授予他“中医专家”的光荣称号。1955年，奉调到京，参加卫生部中医研究院建院初期的医疗和教学工作。一生崇尚“为医者以德为先”，对待患者尽心竭力。在中医儿科的临证工作中历来主张防重于治。并根据中医理论总结出了一套行之有效的调治护理方法，一生造诣颇深。     

双炔失碳酯或丙睾配伍dl-15甲基PGF_(2α)抗早孕的临床比较

本文报告65例双炔失碳酯配伍d1-15甲基PGF_(2α)(以下简称PG)抗早孕结果并与33例丙睾配伍PG抗早孕结果进行比较。结果显示,双炔失碳酯组完全流产59例,占90%;不全流产3例,占5%;失败3例,占5%;总有效率95%。丙睾组完全流产27例,占82%;不全流产6例,占18%;总有效率100%。两组总有效率无显著差异;完全流产率无显著差异;但不全流产率有明显差异(P<0.05)。药流后点滴出血天数,双炔失碳酯组平均为8.1±5.0天;丙睾组平均为18.9±19.1天;两组有明显差别(P<0.05)。双炔失碳酯经阴道给药后无一例发生心、肝、肾功能变化。     

New approach to management of intraaortic balloon pumps in patients with peripheral vascular disease: Case reports of four patients requiring urgent IABP insertion

Four selected cases of emergent IABP insertion in PV patients are presented. After angiographic documentation of critical iliac stenosis, conservative peripheral angioplasty was performed prior to IABP insertion. No patient experienced a peripheral ischemic event associated with IABP use. © 1992 Wiley-Liss, Inc.     

带环后月经过多机制的超微结构研究

带宫内避孕环系常用之节育措施,不正常子宫出血系常见之并发症。本文旨在研究发生出血的机制,提出改进意见,以利于带环妇女的健康,提高带环率。本文对12例带不锈钢环后月经过多妇女的子宫内膜进行超微结构研究,与6例带环而月经正常的妇女对比,结合基础体温、血雌孕激素测定等资料加以讨论。研究结果表明:(1)带环不影响卵巢功能。(2)环周围区与远离区内膜发育的不同步可能是出血过多的重要原因。(3)选择大小适宜的环(宁小勿大)既提高避孕效果又避免出血过多。     

甲壳素制备工艺改进——天然甲壳素的提取

甲壳素制备工艺改进为统一常温进行,操作简便,产品相对收率提高5～10％,同时降低了能耗、机械要求强度、劳动保护条件和生产成本,并经热分析说明产品维持了甲壳素的天然特性,即分子中每结构单元含有1/2分子缔合水,是天然的甲壳素产品并有益于其脱乙酰化物──壳聚糖质量的提高。     

Chemical constituents of Isodon albopilosus (C.Y. Wu et H.W. Li) Hara]

Five compounds have been isolated from the stems and leaves of Isodon albopilosus. On the basis of spectroscopic and chemical data, they were identified as macrocalyxin C, stearic acid, beta-sitosterol, ursolic acid and beta-sitosterol-beta-D-glucoside, respectively.     

中国保健食品行业的现状和发展趋势

<正>保健食品在不同的国家有不同的定义,实施不同的标准、规范和管理,中国的保健食品是由传统的养生概念扩展、衍生而来,其作用机理为:用五味之阴,养五气之阳:平衡阴阳,通经活络、调和气血、滋补脏腑、培