运动性和高血压性心肌肥大对再灌注损伤耐受性的研究

为探讨运动性心肌肥大和高血压心肌肥大对缺血再灌注损伤的差异，本文在大鼠游泳训练心肌肥大和主动脉缩窄性高血压心肌肥大离体心脏灌流模型上，观察它们对缺血再灌注损伤的差异。结果表明，反映组织损伤程度的指标如乳酸脱氢酶漏出，心肌脂质过氧化终产物丙二醛（ＭＤＡ）和心肌钙在高血压心肌肥大的心肌较对照组和运动心肌肥大组皆明显升高，而运动组和对照组则无差异，高血压心肌肥大的程度和运动性心肌肥大的程度基本一致。本研究提示运动性心肌肥大的心肌抗缺血再灌注损伤的能力较高血压心肌肥大者增强。     

小鼠大肠上皮细胞恶性转化中细胞唾液酸粘蛋白的分布及意义

采用Ｎ－甲基－Ｎ－亚硝基脲（ＭＮＵ）直肠灌注诱发小鼠实验性大肠癌，以高铁双胺－奥蓝（ＨＩＤ－ＡＢ）粘蛋白组织化学方法观察诱癌过程中大肠上皮细胞唾液酸粘蛋白组织化学改变及其与大肠上皮细胞形态恶性转化的关系，结果表明第６周起小鼠远端远端结肠呈唾液酸粘蛋白反应上皮细胞数量显著增加，蓝染的唾液酸粘酸粘物质多位于细胞浆，增生的腺腔缘及腺腔分泌物，起始于大肠上皮细胞形态转化之前，峰值出现于原位癌及浸润癌形成前     

糖尿病患者掌、指骨X线测量及其临床意义

６３例糖尿病患者、４０例正常人行左手Ｘ片检查并测量第２掌骨及中指第２指骨骨长（Ｌ）中点的横径（Ｄ）、髓腔宽度（ｄ），以此计算掌、指骨参数，包括Ｄ一ｄ／Ｄ、Ｄ２一ｄ２、Ｄ２一ｄ２／Ｄ２、Ｄ２一ｄ２／ＤＬ。以掌、指骨指数（Ｄ一ｄ／Ｄ）分别低于４３％及３７％为骨质疏松，则糖尿病性骨质疏松症的发病率为４７．６２％。病程长、病情重、失治者易伴发，且多有尿钙、尿磷排泄增加。认为掌、指骨Ｘ线测量可作为判断糖尿病患者有无骨质疏松的一种方法。     

The use of iodinated free fatty acids for assessing fatty acid metabolism

Free fatty acid is a major substrate fuel for normal myocardium. Cardiovascular disease is frequently associated with impairment of fatty acid oxidation. Therefore assessment of fatty acid metabolism may be an important tool for the early detection of myocardial abnormalities and may provide insight into pathologic heart conditions. Although carbon 11-labeled palmitate is a well-established tracer for probing myocardial fatty acid metabolism, a variety of iodinated fatty acid compounds have been introduced for assessing fatty acid metabolism, including straight-chain and branched-chain fatty acid compounds. Straight-chain fatty acid has advantages for measuring fatty acid oxidation on the basis of tracer clearance from the myocardium. Branched-chain fatty acid can be trapped in the myocardium without futher washout and uptake in the myocardium may reflect fatty acid retention and some aspect of fatty acid metabolism. A long tracer retention period makes feasible the acquisition of single-photon emission computed tomographic images. This review examines the characteristics of both types of tracers and our recent clinical experience with β-methyliodophenyl pentadecanoic acid, which has potential for detecting and characterizing both ischemic heart disease and cardiomyopathy.     

胆囊结石患者胆囊粘膜细菌群同步研究

目的:探讨胆道感染时胆囊粘膜细菌生长、细菌变迁,以指导临床合理使用抗生素。方法:采用X、Y、L型培养液检测胆囊结石患者胆囊粘膜细菌。结果:检测胆囊粘膜64例,有细菌生长54例,占83.4％,单种细菌生长40例,两种细茵生长14例。X型细菌生长7种,48例:L型细菌生长4种,16例;厌氧菌生长2种,6例。结论:胆道感染时胆囊粘膜细菌发生变迁,主要以粘质沙雷氏和大肠埃氏杆菌为主。氯霉素和先锋霉素应作为治疗胆道感染首选药物,胆囊粘膜细菌对青霉素应作为治疗胆道感染首选药物,胆囊粘膜细菌对青霉素、氨苄青霉素不敏感。     

The impact of residual renal function and total body water volume on achieving adequate dialysis in CAPD.

AIMS: The objective is to evaluate the impact of residual renal function (RRF) and total body water (TBW) on achieving adequate dialysis. METHODS: Sixty three CAPD patients performing four 2 liter exchanges daily were evaluated for RRF, total weekly Kt/V (TWKt/V), total weekly creatinine clearance (TWCC) and TBW. RESULTS: In patients with residual renal function (N = 41), TWKt/V and TWCC were 2.2 +/- 0.8 and 77.4 +/- 24.5 L, respectively. In patients without RRF (N = 22), TWKt/V was 1.6 +/- 0.4 and TWCC 42.6 +/- 9.2 L. TBW correlated negatively with TWKt/V in the group without RRF (r = -0.75, P<0.001). CONCLUSION: It is not possible for larger patients without RRF treated with CAPD (2L x 4 exchanges) to achieve the acceptable targets for TWKt/V and TWCC due to TBW.     

创伤性髋关节前上方脱位

因创伤导致股骨头脱出于髋臼的病理改变称创伤性髋关节脱位,我们治疗４例前上方脱位,其损伤机制、临床体征和Ｘ线表现均有别于经典的脱位类型。１　临床资料１－１　病例资料　１９９１年９月～１９９４年４月收治髋关节前上方脱位４例,男、女各２例,年龄１９～５２岁。右髋３例,左髋１例。洗澡滑跌伤１例,土石塌方砸伤２例,摩托车撞伤１例。４例均表现患肢外旋、内收、短缩畸形,髋部压痛、弹性固定,髂前上棘前下方或腹股沟部可触及骨性隆起。１－２　Ｘ线表现　Ｘ线片（图１）示股骨头脱出髋臼于髂前上棘前下方、髋臼上缘上方,股…     

胆囊结石患者胆囊胆汁细菌群同步培养分析

为研究胆汁细菌变迁,探讨胆结石核心形成与细菌感染的关系,采用Ｘ,Ｙ及Ｌ型培养液对９８例胆囊结石患者胆汁中细菌进行了检测。结果：９８例之胆汁中有细菌生长者８８例,其中单菌生长５４例,多菌生长３４例;细菌系Ｘ型８３例,Ｌ型２３例,Ｙ型５例,本检测结果提示;胆道感染时胆汁中细菌发生了变迁,主要以粘质沙苗氏菌,大肠杆菌为主。胆囊结石核心形成与细菌感染密切相关。     

乳腺癌中bcl-2蛋白表达的意义

为探讨抑凋亡基因ｂｃｌ－２蛋白表达在乳腺癌中的意义,应用免疫组织化学的方法,对１２５例浸润性乳腺癌患者的石蜡包埋组织切片中ｂｃｌ－２,雌激素受体,孕激素受体及ｐ＾５３基因的表达产物进行检测。结果：ｂｃｌ－２蛋白在浸润性导管癌中的表达为６３．３％,而在浸润性小叶癌中为８８．９％,二者间菜显著性间谍;在浸润性导管癌中,ｂｃｌ－２蛋白的表达与原发癌大小及肿瘤分级呈负相关,与ＥＲ,ＰＲ的表达呈正相关,与ｐ     

丝裂霉素C在腹腔内温热灌注化疗的药代动力学

目的 探讨丝裂霉素Ｃ（ＭＭＣ）用于术中ＩＰＨＰ化疗的药代动力学。方法 １９例中晚期大肠癌病人手术切除肿瘤后,通过加温灌注抗癌仪将溶有８０ｍｇＭＭＣ的生理盐水８０００ｍｌ连续灌注入腹腔,保持腹腔内温度恒定（４３～４５℃）。用高效液相色谱法（ＨＰＬＣ）测定血清、尿、灌注液和局部组织药物浓度。血药浓度用ＧＳＰ程序计算各药动力学参数。结果 血药浓度在灌注终止时达到峰值１２３．８６ｎｇ／ｍｌ,然后迅速下降。