全文获取类型
收费全文 | 7206篇 |
免费 | 321篇 |
国内免费 | 41篇 |
专业分类
耳鼻咽喉 | 79篇 |
儿科学 | 136篇 |
妇产科学 | 88篇 |
基础医学 | 771篇 |
口腔科学 | 112篇 |
临床医学 | 397篇 |
内科学 | 1958篇 |
皮肤病学 | 322篇 |
神经病学 | 413篇 |
特种医学 | 183篇 |
外科学 | 1364篇 |
综合类 | 33篇 |
预防医学 | 173篇 |
眼科学 | 105篇 |
药学 | 516篇 |
中国医学 | 7篇 |
肿瘤学 | 911篇 |
出版年
2023年 | 32篇 |
2022年 | 90篇 |
2021年 | 135篇 |
2020年 | 72篇 |
2019年 | 95篇 |
2018年 | 98篇 |
2017年 | 85篇 |
2016年 | 142篇 |
2015年 | 145篇 |
2014年 | 157篇 |
2013年 | 185篇 |
2012年 | 343篇 |
2011年 | 348篇 |
2010年 | 192篇 |
2009年 | 199篇 |
2008年 | 337篇 |
2007年 | 322篇 |
2006年 | 337篇 |
2005年 | 382篇 |
2004年 | 382篇 |
2003年 | 396篇 |
2002年 | 396篇 |
2001年 | 213篇 |
2000年 | 228篇 |
1999年 | 182篇 |
1998年 | 116篇 |
1997年 | 109篇 |
1996年 | 83篇 |
1995年 | 82篇 |
1994年 | 92篇 |
1993年 | 65篇 |
1992年 | 121篇 |
1991年 | 125篇 |
1990年 | 104篇 |
1989年 | 124篇 |
1988年 | 113篇 |
1987年 | 90篇 |
1986年 | 123篇 |
1985年 | 96篇 |
1984年 | 73篇 |
1983年 | 68篇 |
1982年 | 41篇 |
1981年 | 31篇 |
1979年 | 40篇 |
1978年 | 30篇 |
1977年 | 48篇 |
1975年 | 30篇 |
1972年 | 33篇 |
1971年 | 35篇 |
1970年 | 32篇 |
排序方式: 共有7568条查询结果,搜索用时 15 毫秒
71.
Choi J Koh E Matsui F Sugimoto K Suzuki H Maeda Y Yoshida A Namiki M 《Fertility and sterility》2008,89(5):1177-1182
72.
73.
Iida T Yasuda M Miyazawa M Fujita M Osamura RY Hirasawa T Muramatsu T Murakami M Saito K Mikami M 《Archives of gynecology and obstetrics》2008,277(6):539-546
Material and methods We analyzed the expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) by immunohistochemistry
in ovarian serous and mucinous tumors from the point view of the histological characteristics and acquisition of malignancy.
A total of 102 ovarian tumors were examined, composed of 31 adenomas (serous 17 and mucinous 14), 32 borderline tumors (serous
13 and mucinous 19), and 39 adenocarcinomas (serous 21 and mucinous 18).
Results The overall positive ratios were as follows: HIF-1α, 74% of
adenomas, 91% of borderline tumors, and 100% of adenocarcinomas; and GLUT-1, 68% of adenomas, 95% of borderline tumors, and
100% of adenocarcinomas. Comparing serous tumors and mucinous tumors, there was no significant difference in the positive
ratios of HIF-1α and GLUT-1 of adenomas, borderline tumors, and adenocarcinomas. However, both markers were more strongly
expressed in serous adenocarcinomas (HIF-1α, 3 + 100%; GLUT-1, 3 + 76%) than in mucinous adenocarcinomas (HIF-1α, 3 + 61%;
GLUT-1, 3 + 28%). The results of immunoblotting and mRNA expression level analyses corresponded with those of immunohistochemical
expression profiles. DNA binding assay also demonstrated that HIF-1 is more commonly activated in serous adenocarcinomas than
in mucinous adenocarcinomas.
Conclusion HIF-1α and GLUT-1 expressions seemed to be coordinated to adapt ovarian tumor cells into hypoxic conditions in close association
with the acquisition of malignancy. We consider that the relatively strong expression of both markers in serous tumors compared
with mucinous tumors is related to the difference in their histological characteristics. 相似文献
74.
Miyamoto T Koh E Sakugawa N Sato H Hayashi H Namiki M Sengoku K 《Journal of assisted reproduction and genetics》2008,25(11-12):553-557
Purpose
To investigate whether defects in human PRDM9, CDK2 and PSMC3IP are associated with azoospermia Mutational analysis was performed in Japanese patients with azoospermia caused by meiotic arrest.Methods
Mutational screening of the coding regions of human PRDM9, CDK2 and PSMC3IP was done by direct sequencing using genomic DNA from 18 Japanese patients. Statistical analysis of the detected coding single nucleotide polymorphisms (cSNPs) in patients and normal control men was then carried out.Results
One cSNP was detected in CDK2 and PSMC3IP. There were no significant differences in genotype distribution and allele frequencies between the patient and control groups in these two genes. However, three novel cSNPs were detected in the PRDM9. The genotype and allele frequencies of heterozygotes in SNP2 and SNP3 of PRDM9 were significantly higher in the patient group than in the control group.Conclusion
We found a possible association between PRDM9 and azoospermia by meiotic arrest.75.
76.
Teruhiko Imamura Toshihide Izumida Masakazu Hori Shuhei Tanaka Koichiro Kinugawa 《The Journal of international medical research》2022,50(7)
Cardiac amyloidosis usually presents with diastolic dysfunction, but sometimes systolic dysfunction develops, particularly at its advanced stage. However, the therapeutic strategy for patients with cardiac amyloidosis and systolic dysfunction remains unknown. We report a 77-year-old man who was diagnosed with wild-type cardiac amyloidosis and systolic dysfunction with a left ventricular ejection fraction of 27%. Following 6-month medical therapy of tafamidis 80 mg and neurohormonal blockers (carvedilol 5.0 mg, enalapril 2.5 mg, and spironolactone 25 mg), the left ventricular ejection fraction improved to 55%. Tafamidis-incorporated neurohormonal blocker therapy might be a promising strategy to facilitate cardiac reverse remodeling in patients with cardiac amyloidosis and systolic dysfunction. 相似文献
77.
Nobuyuki Nishikawa Rie Yago Yuichiro Yamazaki Hiromitsu Negoro Mari Suzuki Masaaki Imamura Yoshinobu Toda Kazunari Tanabe Osamu Ogawa Akihiro Kanematsu 《BMC urology》2015,15(1)
Background
To investigate the expression of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor 1 (PTH1R) in clinical specimens of normal and diseased bladders. PTHrP is a unique stretch-induced endogenous detrusor relaxant that functions via PTH1R. We hypothesized that suppression of this axis could be involved in the pathogenesis of bladder disease.Methods
PTH1R expression in clinical samples was examined by immunohistochemistry. Normal kidney tissue from a patient with renal cancer and bladder specimens from patients undergoing ureteral reimplantation for vesicoureteral reflux or partial cystectomy for urachal cyst were examined as normal control organs. These were compared with 13 diseased bladder specimens from patients undergoing bladder augmentation. The augmentation patients ranged from 8 to 31 years old (median 15 years), including 9 males and 4 females. Seven patients had spinal disorders, 3 had posterior urethral valves and 3 non-neurogenic neurogenic bladders (Hinman syndrome).Results
Renal tubules, detrusor muscle and blood vessels in normal control bladders stained positive for PTH1R. According to preoperative urodynamic studies of augmentation patients, the median percent bladder capacity compared with the age-standard was 43.6% (range 1.5–86.6%), median intravesical pressure at maximal capacity was 30 cmH2O (range 10–107 cmH2O), and median compliance was 3.93 ml/cmH2O (range 0.05–30.3 ml/cmH2O). Detrusor overactivity was observed in five cases (38.5%). All augmented bladders showed negative stainings in PTH1R expression in the detrusor tissue, but positive staining of blood vessels in majority of the cases.Conclusions
Downregulation of PTH1R may be involved in the pathogenesis of human end-stage bladder disease requiring augmentation. 相似文献78.
79.
80.
K Yokono K Amano K Suenaga J Hari K Shii S Yaso K Yonezawa Y Imamura S Baba 《Diabetes research and clinical practice》1985,1(6):315-321
Immunotherapeutic intervention has been studied in non-obese diabetic (NOD) mice. Twenty-five male NOD mice aged 6 weeks were treated with anti-mouse T lymphocyte serum (ATS), N-(2-carboxyphenyl)-4-chloroanthranilic acid disodium salt (CCA); a non-specific immunostimulant which seems to potentiate the suppressor T cell activity, and antiserum raised against previously reported monoclonal antibody 3A4 to the surface of islet cells. Pancreatic islets from NOD mice sacrificed at 12 weeks of age were scored morphologically for the severity and the frequency of insulitis. Both the severity of insulitis in each islet and the frequency of insulitis-positive islets in each pancreas were reduced in the groups treated with ATS (group B), antiserum to 3A4 (group D) and a combination of antiserum plus CCA (group E) in comparison with other groups (control: group A and CCA: group C). At 8 weeks of age, the binding capacities of sera to insulinoma cells measured by protein A radioligand assay were significantly decreased in the groups treated with antiserum to 3A4 (groups D and E) as compared with those in the other groups. These results suggest that both ATS and antiserum to 3A4 prevent the occurrence and the progress of insulitis in NOD mice, but the immunosuppressive mechanisms differ from each other; therefore, combination therapy with these suppressants may be more effective in the prevention of Type 1 diabetes mellitus. 相似文献