全文获取类型
收费全文 | 2708篇 |
免费 | 155篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 70篇 |
妇产科学 | 46篇 |
基础医学 | 340篇 |
口腔科学 | 90篇 |
临床医学 | 214篇 |
内科学 | 581篇 |
皮肤病学 | 67篇 |
神经病学 | 302篇 |
特种医学 | 204篇 |
外科学 | 334篇 |
综合类 | 21篇 |
预防医学 | 138篇 |
眼科学 | 102篇 |
药学 | 177篇 |
中国医学 | 12篇 |
肿瘤学 | 166篇 |
出版年
2023年 | 31篇 |
2022年 | 40篇 |
2021年 | 80篇 |
2020年 | 41篇 |
2019年 | 62篇 |
2018年 | 88篇 |
2017年 | 59篇 |
2016年 | 70篇 |
2015年 | 69篇 |
2014年 | 99篇 |
2013年 | 110篇 |
2012年 | 175篇 |
2011年 | 220篇 |
2010年 | 119篇 |
2009年 | 93篇 |
2008年 | 167篇 |
2007年 | 203篇 |
2006年 | 197篇 |
2005年 | 160篇 |
2004年 | 148篇 |
2003年 | 161篇 |
2002年 | 132篇 |
2001年 | 24篇 |
2000年 | 23篇 |
1999年 | 24篇 |
1998年 | 17篇 |
1997年 | 16篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 11篇 |
1993年 | 9篇 |
1992年 | 24篇 |
1991年 | 17篇 |
1990年 | 19篇 |
1989年 | 9篇 |
1988年 | 14篇 |
1987年 | 16篇 |
1986年 | 13篇 |
1985年 | 11篇 |
1984年 | 13篇 |
1983年 | 11篇 |
1982年 | 6篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1973年 | 5篇 |
1971年 | 4篇 |
排序方式: 共有2875条查询结果,搜索用时 406 毫秒
41.
42.
Shou Soeda Kaori Yamada Miho Ohsugi 《Genes to cells : devoted to molecular & cellular mechanisms》2013,18(10):850-858
Mammalian oocytes are arrested at metaphase II due to high MAP kinase activity. After fertilization, oocytes resume meiosis, leading to female chromosome segregation, polar body emission and pronuclear (PN) formation. Previous biochemical studies showed that MAP kinase activity remained high for several hours after fertilization and began to decrease in parallel with PN formation. It has been thought that MAP kinase activity is incompatible with PN formation, and its inactivation is required for the initiation of PN formation in mammalian oocytes. In this study, we revisited this hypothesis by examining MAP kinase activity and PN formation in individual mouse oocytes using cytological analysis. We showed that MAP kinase activity in oocytes could be evaluated using phospho‐ERK1/2 immunofluorescent staining. Co‐immunofluorescent staining of phospho‐ERK1/2 and nuclear pore components showed that PN formation preceded MAP kinase inactivation and could be initiated while MAP kinase activity was still high. Moreover, artificial inactivation of MAP kinase or its downstream target, ribosomal S6 kinase, accelerated but did not rapidly induce PN formation. Our results show that although the MAP kinase pathway negatively regulates PN formation, its inactivation is neither necessary nor sufficient for PN formation. These results suggest the involvement of other essential factor(s) in this process. 相似文献
43.
Eiji Wada Mitsuru Fukui Kazuhisa Takahashi Daisaku Takeuchi Hiroshi Hashizume Masahiko Kanamori Noboru Hosono Tsukasa Kanchiku Yuichi Kasai Miho Sekiguchi Shin-ichi Konno Mamoru Kawakami Kazuo Yonenobu 《Journal of orthopaedic science》2019,24(1):57-61
Background
In 1999, the Japanese Orthopaedic Association decided to develop a new Cervical Myelopathy Evaluation Questionnaire (JOACMEQ). The final version of the JOACMEQ, comprising 24 questions and five domains (cervical spine function (CF); upper extremity function (UF); lower extremity function (LF); bladder function (BF); and quality of life (QOL)), was established after three nationwide investigations. The fourth investigation, reported in this paper, was performed to confirm the responsiveness of the questionnaire.Methods
A total of 137 patients with cervical myelopathy were included in the study. Each patient was interviewed twice using the JOACMEQ before and after treatment. At the second interview, the patients self-rated their condition in five domains for “worse,” “somewhat worse,” “no change,” “somewhat better,” or “better,” and these scores were defined as the external assessment rating. The difference of the points in five domains between the first and the second interview was calculated against each external assessment. Based on the results, substantial clinical benefit (SCB) thresholds for the JOACMEQ were determined.Results
The statistically significant median values of the acquired points were 17.5 for CF, 16.0 and 21.0 for UF, 27.0 and 20.5 for LF, 13.0 for BF, and 29.0 for QOL. After consideration of the results, the committee decided that an acquired point ≥20 could be interpreted as representing an SCB threshold for the JOACMEQ.Conclusion
We have concluded that a treatment can be judged to be effective for a patient if 1) The patient give all answers for the questions necessary to calculate the functional score of a domain and an increase of ≥20 points is obtained for that score, or 2) The functional score after treatment is > 90 points even if the answer for the unanswered questions was supposed to be the worst possible choice. 相似文献44.
Miho Tanaka Carol Strong Sunmin Lee Hee-Soon Juon 《Journal of immigrant and minority health / Center for Minority Public Health》2013,15(4):779-787
This study examines how different information sources relate to Health Belief Model constructs, hepatitis B virus (HBV) knowledge, and HBV screening. The Maryland Asian American Liver Cancer Education Program administered a survey of 877 Asian immigrants. The most common sources of information identified by the multiple-answer questions were newspapers (39.8 %), physicians (39.3 %), friends (33.8 %), TV (31.7 %), and the Internet (29.5 %). Path analyses—controlling for age, sex, educational level, English proficiency, proportion of life in U.S., health insurance coverage, and family history of HBV infection—showed that learning about HBV from physicians had the strongest direct effect; friends had a marginal indirect effect. Perceived risk, benefits, and severity played limited roles in mediation effects. Path analysis results differed by ethnicity. Physician-based HBV screening intervention would be effective, but should be complemented with community health campaigns through popular information sources for the uninsured. 相似文献
45.
Tsubasa Hiraki Miho Hatanaka Akiko Arimura Hisao Kawahira Mari Kirishima Ikumi Kitazono Michiko Horinouchi Michiyo Higashi Takuro Kanekura Akihide Tanimoto 《Journal of cutaneous pathology》2020,47(2):154-160
Nivolumab and pembrolizumab are humanized IgG4 monoclonal antibodies against programmed cell death 1 (PD-1). Although these agents are effective in treating advanced melanoma, non-small-cell lung carcinoma, and other types of cancers, various adverse events have been reported. Cutaneous adverse events are particularly prevalent and, while granulomatous/sarcoid-like reactions are uncommon, they are increasingly recognized as immune-related adverse events associated with immune checkpoint inhibitors. Herein, we report two cases of granulomatous/sarcoid-like reaction with foreign material, mimicking metastatic malignancy after PD-1 inhibitor treatment. Clinicians should be aware of the existence of cutaneous lesions and perform biopsy if needed to prevent misdiagnosis and unnecessary adjustments to immunotherapy. 相似文献
46.
Extremely early onset of ranitidine action on human histamine H2 receptors expressed in HEK293 cells
Fukushima Y Ishikawa T Saitoh T Tateishi K Ogihara T Fujishiro M Shojima N Honda M Kushiyama A Anai M Sakoda H Ono H Onishi Y Otsuka H Katagiri H Nagai R Omata M Asano T 《Digestion》2003,68(2-3):145-152
BACKGROUND/AIMS: Histamine H2 receptor antagonists are considered to exert their effects on gastric acid secretion more rapidly than proton pump antagonists. However, there are no reports concerning the direct interaction of a histamine H2 receptor antagonist with the human H2 receptor in terms of onset of action. This study aims to characterize how rapidly famotidine and ranitidine, the most widely used histamine H2 receptor antagonists, interact with the human histamine H2 receptor. METHODS: HEK293 cell lines, stably expressing human histamine H2 receptors, were obtained. The dose- and time-dependent effects of famotidine and ranitidine on [3H]-tiotidine binding and histamine-stimulated cAMP production were analyzed. RESULTS: Ranitidine inhibited both [3H]-tiotidine binding and histamine-stimulated cAMP production more promptly than did famotidine. Inhibition of histamine-stimulated cAMP production by Cmax doses of famotidine (20 mg p.o.) and ranitidine (150 mg p.o.) peaked by 15 and 2 min, respectively. [3H]-tiotidine binding was not saturated by 60 min at the famotidine Cmax, while the ranitidine Cmax had produced saturation by 15 min. CONCLUSION: Ranitidine inhibits the human histamine H2 receptor very rapidly. 相似文献
47.
Nishikawa K Matsuoka K Watanabe M Igai K Hino K Hatano K Yamada A Abe N Terunuma D Kuzuhara H Natori Y 《The Journal of infectious diseases》2005,191(12):2097-2105
Shiga toxin (Stx) is a major virulence factor of Stx-producing Escherichia coli. Recently, we developed a therapeutic Stx neutralizer with 6 trisaccharides of globotriaosyl ceramide, a receptor for Stx, in its dendrimer structure (referred to as "SUPER TWIG [1]6") to function in the circulation. Here, we determined the optimal structure of SUPER TWIG for it to function in the circulation and identified a SUPER TWIG with 18 trisaccharides, SUPER TWIG (2)18, as another potent Stx neutralizer. SUPER TWIGs (1)6 and (2)18 shared a structural similarity, a dumbbell shape in which 2 clusters of trisaccharides were connected via a linkage with a hydrophobic chain. The dumbbell shape was found to be required for formation of a complex with Stx that enables efficient uptake and degradation of Stx by macrophages and, consequently, for potent Stx-neutralizing activity in the circulation. We also determined the binding site of the SUPER TWIGs on Stx. 相似文献
48.
Furuta T Akiyama M Kato Y Nishio O 《Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases》2003,77(2):89-94
A party of 57 people dined together in a restaurant in Hamamatsu City on December 11, 2001. The next day, 22 of them developed symptoms of acute gastroenteritis, such as diarrhea, vomiting, and fever. Examination of 4 fecal specimens from these patients by ELISA for Norovirus (Norwalk-like virus, NV) detected both genogroup I (GI) and genogroup II (GII) NV in all the 4 specimens. In addition, RT-PCR and real-time PCR methods for NV detected the NV gene. Approximately one month after the outbreak of the food poisoning (acute gastroenteritis) by NV, 4 individuals in the same party developed type A hepatitis. Both RT-PCR and real-time PCR methods for hepatitis A virus (HAV) detected the HAV gene in their fecal specimens. The party of these patients ate purple Washington clam (Saxidomus purpuratus, imported from China) steamed with red pepper. Since this food appeared to have caused the viral infections, the one with the same lot number was subjected to viral examinations, which successfully detected the NV GI, NV GII, and HAV genes. These results led to the conclusion that the clam contaminated with NV and HAV had caused the food poisoning. The DNA sequences of the NV detected in the patients and the clam had 74 to 99% homology, indicating strains of various genotypes. All the strains of HAV that were derived from the patients and the clam were genotype 1A, and these sequences had over 95% homology, but were not completely identical. This outbreak led to the demonstration of imported fishery products as a cause of type A hepatitis, and indicated the need for guiding and enlightening people on the importance of adequate cooking of bivalves. 相似文献
49.
50.
Usefulness of measurement of reticulated platelets for diagnosis of idiopathic thrombocytopenic purpura. 总被引:2,自引:0,他引:2
Miho Sakakura Hideo Wada Yasunori Abe Junji Nishioka Hiroaki Tomatsu Yukio Hamaguchi Shinichiro Oguni Hiroshi Shiku Tsutomu Nobori 《Clinical and applied thrombosis/hemostasis》2005,11(3):253-261
Reticulated platelets (RP) and large platelets (LP) were measured by an automated hematology analyzer (modified R-2000) in 287 healthy volunteers and 131 patients with thrombocytopenia or thrombocytosis. RP was significantly higher in patients with idiopathic thrombocytopenic purpura (ITP), especially in active phase, while RP was markedly lower in patients with essential thrombocytosis (ET) or chronic myelocytic leukemia (CML). LP was significantly higher in patients with ITP, especially in active phase, while LP was markedly lower in patients with aplastic anemia (AA), ET, or CML. In ITP, RP and LP were significantly higher in patients positive for anti-glycoprotein (Gp) IIb/IIIa antibody. RP and LP were poorly correlated with platelet-associated IgG (PAIgG). RP and LP were poorly correlated with plasma thrombopoietin levels, and negatively correlated with platelet count. These results show that RP reflects the pathology of thrombocytopenic disorders, and that measurement of RP is useful for the differential diagnosis and analysis of platelet kinetics. 相似文献