血管成形术和支架辅助血管成形术治疗颅内动脉粥样硬化的报告标准（下）

本刊经Philip M．Meyers博士代表写作组授权,将“ Reporting standards for angioplasty and stent-assisted angioplasty for intracranial atherosclerosis”译为中文在本刊刊登。标准中对患者的选择、颅内动脉狭窄程度的判断、最佳内科治疗、围手术期处理、血管内治疗、术后并发症等,进行了规范化总结,拟为今后的临床试验和研究的规范化确定标准,以保证结果的可比性,对神经介入医师具有重要的指导意义。     

Mental health status of Japanese‐Brazilian children at Brazilian schools in Japan

Introduction: Although the mental health of Japanese‐Brazilian children in Japan has become a matter of concern, few studies have employed a psychological approach and no research of these children's mental health has previously been performed at Brazilian schools in Japan. Methods: The present study used a sample of 241 children aged 4 to 10 years at five Brazilian schools in Japan. The Strength and Difficulties Questionnaire was applied for their guardians and teachers to detect the probable cases of mental disorders. Demographic data of the children were also collected from their guardians and a further analysis was made to determine the risk factors of the probable cases of conduct disorders. Results: The proportion of probable cases was 0.8% for emotional disorders, 9.1% for conduct disorders, 2.1% for hyperactivity disorders, and 10.0% for any psychiatric disorder. The detected risk factors of conduct disorders were male sex, 8 to 10 years of age, living without a father, and not talking often with their teacher. Discussion: The proportions of probable cases of mental disorders were not as high compared to general prevalence ranges or earlier studies. Considering the school and home situation of the children, living without a father seemed to be the foremost risk factor of conduct disorders.     

Development of 13 microsatellite loci in the spotted snow skink Niveoscincus ocellatus (Squamata: Scincidae)

Thirteen polymorphic tri- and tetra-nucleotide microsatellites are reported for the spotted snow skink (Niveoscincus ocellatus) from Tasmania. Variation was assessed among 40 individuals collected from a single locality. Most loci had 12–19 alleles, although one had more than 20 alleles, while observed heterozygosities ranged between 0.25 and 0.93. Eleven loci were also polymorphic in the congeneric species N. metallicus (N = 16 collected from a single locality).     

Innate immune genes synergize to predict increased risk of chronic disease in hepatitis C virus infection

Hepatitis C is a common infection with significant morbidity and mortality, and only a minority of patients successfully clear the infection. Identification of factors that influence disease progression in HCV infection is difficult owing to the lack of well-defined patient cohorts. However, recent evidence supports a role for the innate immune system in virus clearance. In this study, we investigated innate immune genes for their contribution to disease progression in a unique cohort of well-controlled HCV-infected patients. The Irish cohort of HCV patients is uniquely homogenous; patients were infected with a single genotype of HCV from contaminated anti-D Ig. We genotyped 543 infected patients, including 247 patients who spontaneously resolved infection, for natural killer (NK) cell-associated killer cell Ig-like receptors (KIR) genes and the recently reported IL28B (IFNλ3) SNP. The NK cell gene KIR2DS3 was significantly increased in patients with chronic infection [odds ratio (OR) 1.90, 95% confidence interval (CI) 1.25-2.90, P < 0.002]. The IL28B "T" allele was also significantly increased in chronically infected patients (OR 7.38, 95% CI 4.93-11.07, P < 10(-8)). The presence of both markers synergized to significantly increase the risk of chronic infection over either factor alone (OR 20.11, 95% CI 9.05-44.68, P < 10(-7)). In functional experiments, we found that IL28A significantly inhibited IFN-γ production by NK cells. Thus, we demonstrate a functional link between NK cells and type 3 IFN. Our findings may contribute to the development of a prognostic test for HCV and identify therapeutic strategies for the clinical management of HCV-infected patients.     

红细胞多态性的基因分型

红细胞多态性在DNA水平的分型是输血医学的重大进展,其将对输血服务产生重大影响.最近,国际输血协会和国际血液学标准化委员会就此问题组织三次研讨会结果表明,可为分子血型基因分型设定非常高的标准.随着基因分型用于红细胞多态性研究的报道迅速增加,引起了国际论坛对其进展评价的关注.为了获得必要的信息,该领域的专家被问及以下问题:     

Flat adenoma in colon: Two decades of debate

The existence of flat adenomas in the colon is well recognized. Whether they represent a distinct disease with a pathogenetic pathway different from that of the classical adenoma-carcinoma sequence in colorectal tumorigenesis and have higher malignant potential remains a matter of debate. To review the epidemiology, clinical features, detection and management of flat and depressed (non-polypoid) colonic neoplasm, we performed a thorough literature review on studies focusing on the prevalence, histological features, genetics, detection and treatment of flat and depressed (non-polypoid) colonic neoplasm. A high percentage of severe dysplasia in flat colonic adenomas has not been consistently demonstrated. Their malignant potential appears to be size-dependent. Flat adenomas are found to have a lower incidence of major genetic abnormalities involved in the classical adenoma-carcinoma sequence and that has raised suspicions that they may have a different pathogenesis. The depressed type of colorectal carcinoma is uncommon but shows more aggressive behavior. More advanced colonoscopic techniques, such as chromoendoscopy, may enhance the detection of small and inconspicuous colonic neoplastic lesions that lack a protruding configuration. It is essential for endoscopists to appreciate the existence and clinical significance of flat and depressed colonic lesions as an important variant of colonic neoplasms so that the goal of reducing colorectal carcinoma incidence by polypectomy can be better achieved.     

Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia

Background  

Microglia are considered a major target for modulating neuroinflammatory and neurodegenerative disease processes. Upon activation, microglia secrete inflammatory mediators that contribute to the resolution or to further enhancement of damage in the central nervous system (CNS). Therefore, it is important to study the intracellular pathways that are involved in the expression of the inflammatory mediators. Particularly, the role of the phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) and glycogen synthase kinase-3 (GSK-3) pathways in activated microglia is unclear. Thus, in the present study we investigated the role of Akt and its downstream pathways, GSK-3 and mTOR, in lipopolysaccharide (LPS)-activated primary rat microglia by pharmacological inhibition of these pathways in regard to the expression of cyclooxygenase (COX)-2 and microsomal prostaglandin E synthase-1 (mPGES-1) and to the production of prostaglandin (PG) E₂ and PGD₂.