Ischemic injury to the conducting system of the heart. Involvement of myocardial lysosomes.

The conducting system was studied in an in situ perfused swine heart preparation with reduced coronary flow (ischemia) using perfusate containing high and low levels of glucose (26.6 versus 8.6mM) with and without insulin. Coronary flow was maintained at normal levels for 60 minutes in control hearts. In ischemic hearts flow was reduced to about 50 percent of control levels for 30 minutes. Ultrastructural studies documented only subtle modifications of Purkinje fibers in ischemic hearts. Glycogen depletion and disruption of cell junctions were observed in some fibers. One consistent finding was the activation of the lysosomal system. The outer membranes of primary lysosomes appeared herniated and in some cases disrupted, and small vesicles containing hydrolytic enzymes were seen in association with the Golgi apparatus and larger primary lysosomes. Specimens prepared for the demonstration of acid phosphatase indicated a redistribution of hydrolytic enzymes in Purkinje fibers with a depostion of acid hydrolases in smaller lysosomal vesicles, the transverse and side-to-side junctions between cells, and occasionally in the sarcoplasmic reticulum. Enriched perfusate containing high levels of glucose with insulin appeared to have no therapeutic effects in terms of the structure of the Purkinje fibers. The results suggest that alterations in the lysosomal system may be one of the earliest structural changes which occur in oxygen-deficient hearts.     

Mechanisms of human CD5 modulation and capping induced by murine monoclonal antibody T101

We have previously demonstrated that the murine monoclonal antibody T101 induces antigenic modulation when infused into patients with chronic lymphocytic leukemia and cutaneous T-cell lymphoma. In this paper, we extend our studies of T101-induced modulation and compare it to T101-induced capping. We found that, in contrast to antigenic modulation, capping occurred only in the presence of secondary anti-mouse IgG antisera and was altered by drugs that affect the cellular cytoskeleton or energy metabolism. F(ab')2 fragments of T101 induced antigenic modulation with kinetics similar to those of intact T101, but Fab-induced modulation proceeded more slowly and required the continual presence of Fab throughout the incubation. Experiments with radioiodinated T101 demonstrated that initial internalization of the antibody is followed by rapid efflux of intact, immunoreactive T101 from the cells. These data indicate important differences between capping and modulation and suggest that these two phenomena proceed by different mechanisms. More importantly, the data have implications for the potential therapeutic use of monoclonal antibody immunoconjugates.     

Endoscopic versus open surgical treatment of carpal tunnel syndrome

The impact of CTS is significant as evidenced by the fact that only back injuries result in greater rates of employee absenteeism in the workplace. CTR is now the most commonly performed surgical procedure in the United States. Earlier efforts using open surgical techniques were associated with significant morbidity, which some would argue is greater than that associated with the disease itself. The addition of endoscopy to surgeon's armamentarium offers the promise of decreased morbidity associated with the surgical treatment of CTS. Evidence indicates that when compared with open CTR, endoscopic CTR results in earlier achievement of patient satisfaction and functional outcomes. As a result, it is becoming clear that endoscopic surgery is a safe and effective method of treating CTS.     

Central placement of the screw in simulated fractures of the scaphoid waist: a biomechanical study

BACKGROUND: Recent reports on internal fixation of acute fractures of the scaphoid waist have demonstrated higher rates of central placement of the screw when cannulated screws were used than when noncannulated screws were used. This cadaveric study was designed to determine whether central placement in the proximal fragment of the scaphoid offers a biomechanical advantage. METHODS: Eleven matched pairs of scaphoids were removed from fresh cadaveric wrists. Each scaphoid was placed in a custom manufactured jig that was used to create reproducible central and eccentric positioning of the guidewire. Then a linear osteotomy was made followed by placement of a Herbert-Whipple cannulated screw to fix the osteotomy. The specimen was then potted in a holder with use of polymethylmethacrylate with a Kirschner wire passed through the proximal end of the scaphoid and placed into a fixture with a pneumatically driven plunger resting on the surface of the distal pole. The load acting through the plunger was measured with use of a load-cell, and its excursion was measured with use of a linear variable differential transformer. Stiffness, load at 2 mm of displacement, load at failure, and mechanism of failure were measured, and the two groups were compared with regard to stiffness and strength. RESULTS: Central placement of the screw in the proximal fragment of the scaphoid had superior results compared with those after eccentric positioning of the screw. Fixation with central placement of the screw demonstrated 43% greater stiffness (12.7 N per mm compared with 8.9 N per mm; p < 0.01), 113% greater load at 2 mm of displacement (126 N compared with 59.1 N; p < 0.01), and 39% greater load at failure (712 N compared with 513 N; p > 0.05). CONCLUSIONS AND CLINICAL RELEVANCE: Central placement of the screw in the proximal fragment of the scaphoid offers a biomechanical advantage in the internal fixation of an osteotomy of the scaphoid waist. Clinical efforts and techniques that facilitate central placement of the screw in the fixation of fractures of the scaphoid waist should be encouraged.