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41.
Tomohiro Kunishige Tomoyoshi Takayama Sohei Matumoto Kohei Wakatsuki Koji Enomoto Tetsuya Tanaka Kazuhiro Migita Masamitsu Kuwahara Hiroshi Iioka Yoshiyuki Nakajima 《International journal of surgery case reports》2013,4(9):793-797
INTRODUCTIONIn the present paper, we show a rare case of the large abdominal wall defect and enterocutaneous fistulas after the tension free repair using prostheses for incisional hernia.PRESENTATION OF CASEThe patient, a 70-year-old man, had a history of a hemicolectomy for a perforating colon cancer, complicated by a large incisional hernia that was closed primarily but recurred. Three years later, the hernia was repaired at the time of a second colectomy using a Composix Kugel Patch. His course was complicated by a chronic postoperative wound infection with eventual development of enterocutaneous fistulas. The patient was successfully treated with extirpation of the prosthesis, resection of the fistulized bowel, and placement of a tensor fasciae latae myocutaneous flap.DISCUSSIONEnterocutaneous fistulas are a known complication of incisional hernia repairs using prostheses. Additional clinical data are required to confirm the safety and efficacy of this procedure as it becomes more widely adopted.CONCLUSIONExtirpation of the prosthesis should be performed without delay to prevent serious complications. Reconstruction with a tensor fasciae latae myocutaneous flap was useful for the large abdominal wall defect. 相似文献
42.
Hirotaka Tanaka Masamitsu Shimazawa Masafumi Takata Hideo Kaneko Kazuhiro Tsuruma Tsunehiko Ikeda Hitoshi Warita Masashi Aoki Mitsunori Yamada Hitoshi Takahashi Isao Hozumi Hiroshi Minatsu Takashi Inuzuka Hideaki Hara 《Journal of neurology》2013,260(7):1782-1797
The diagnosis of amyotrophic lateral sclerosis (ALS) is difficult due to lack of definitive biomarkers. Our aim was to identify characteristic serum protein patterns that could provide candidate biomarkers for ALS. We divided mutant superoxide dismutase-1 (SOD1)H46R rats into three groups based on disease progression: pre-symptom (90 days), onset, and end-stage. After separation of serum proteins using two-dimensional electrophoresis, we selected clear protein spots and identified two candidate proteins—inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) and glutathione peroxidase 3 (Gpx3). The 120 kDa ITIH4 increased at the onset of the disease and the 85 kDa ITIH4, a cleaved form, at the end-stage in the sera of the SOD1H46R rats. Expression of the 85 kDa ITIH4 was substantial in ALS compared with controls or patients with muscular dystrophy, Alzheimer diseases, or Parkinson diseases. The Gpx3 protein levels in the sera of SOD1H46R rats were upregulated pre-symptom and gradually decreased as the disease progressed. The Gpx3 protein levels were lower in the sera of the patients with ALS than in other diseases. These results indicate that ITIH4 and Gpx3 are potential biomarkers for ALS. 相似文献
43.
Natsumi Furuta Kouki Makioka Yukio Fujita Masaki Ikeda Masamitsu Takatama Masaaki Matsuoka Koichi Okamoto 《Neuropathology》2013,33(4):397-404
Overexpression of BTBD10 (BTB/POZ domain‐containing protein 10) suppresses G93A‐superoxide dismutase 1 (SOD1)‐induced motor neuron death in a cell‐based amyotrophic lateral sclerosis (ALS) model. In the present study, paraffin sections of spinal cords from 13 patients with sporadic ALS and 10 with non‐ALS disorders were immunostained using a polyclonal anti‐BTBD10 antibody. Reduced BTBD10 expression in the anterior horn cells was more frequent in spinal cords from ALS patients than in cords from patients with non‐ALS disorders. We further investigated the relationship between the level of BTBD10 immunoreactivity and the morphology of the Golgi apparatus (GA) and the presence of phosphorylated TAR‐DNA‐binding protein 43 (pTDP‐43). Mirror sections of spinal cords from five sporadic ALS cases were immunostained with antibodies against BTBD10 and trans‐Golgi‐network (TGN)‐46 or pTDP‐43. Whereas 89.7–96.5% of the neurons with normal BTBD10 immunoreactivity showed normal GA morphology and no pTDP‐43 cytoplasmic aggregates, 86.2–94.3% of the neurons with reduced BTBD10 expression showed GA fragmentation and abnormal pTDP‐43 aggregates. These findings suggest that reduced BTBD10 expression is closely linked to the pathogenesis of sporadic ALS. 相似文献
44.
Trials of vaccines for pancreatic ductal adenocarcinoma: Is there any hope of an improved prognosis?
Toru?MizuguchiEmail author Toshihiko?Torigoe Fukino?Satomi Hiroaki?Shima Goro?Kutomi Shigenori?Ota Masayuki?Ishii Hiroshi?Hayashi Sumiyo?Asakura Yoshihiko?Hirohashi Makoto?Meguro Yasutoshi?Kimura Toshihiko?Nishidate Kenji?Okita Masaho?Ishino Atsushi?Miyamoto Masamitsu?Hatakenaka Noriyuki?Sato Koichi?Hirata 《Surgery today》2016,46(2):139-148
Pancreatic tumors are chemoresistant and malignant, and there are very few therapeutic options for pancreatic cancer, as the disease is normally diagnosed at an advanced stage. Although attempts have been made to develop vaccine therapies for pancreatic cancer for a couple of decades, none of the resultant protocols or regimens have succeeded in improving the clinical outcomes of patients. We herein review vaccines tested within the past few years, including peptide, biological and multiple vaccines, and describe the three sets of criteria used to evaluate the therapeutic activity of vaccines in solid tumors. 相似文献
45.
Tomohiro Oba Junichi Hasegawa Tatsuya Arakaki Hiroko Takita Masamitsu Nakamura Akihiko Sekizawa 《The journal of maternal-fetal & neonatal medicine》2016,29(21):3449-3453
Objective: Hemorrhagic shock is a relatively common occurrence in the postpartum period. In our hospital, we performed abdominal ultrasonography using the focused assessment with sonography for obstetrics (FASO) technique (a modified version of FAST). The aim of the present study was to determine the reference values for the ultrasonographic findings to establish the criteria for the diagnosis of a postpartum hemorrhage and severe shock using the FASO.Methods: The present prospective cohort study included all postpartum women who vaginally delivered singleton infants. Abdominal ultrasonography was performed after delivery. The observation points of ultrasonography were as follows: (1) the diameter of the intrauterine cavity, (2) the pouch of Douglas, (3) Morison’s pouch, (4) between the spleen and kidney, and (5) the diameter of the inferior vena cava.Results: One hundred and eighty-two postpartum women were included in this study. The mean uterine cavity was 9.8?±?7.3?mm. An echo-free space in the pouch of Douglas was observed in three cases, in one case in Morison’s pouch, and not observed between the spleen and kidney. A negative correlation was found between the volume of bleeding and IVCi (p?=?0.0008, r2=??0.061) and IVCe (p?<?0.0001, r2=??0.106).Conclusions: The present study establishes criteria that can be used to diagnose a postpartum hemorrhage or severe shock using the FASO. 相似文献
46.
Terashita T Saito S Miyawaki K Hyodo M Kobayashi N Shimokawa T Saito K Matsuda S Gyo K 《Neuroscience research》2007,57(3):372-378
Prosaposin, the precursor of the sphingolipid hydrolase activator proteins called saposins A, B, C, and D, is abundant in the nervous system and muscles. Besides its role as the precursor of saposins, prosaposin is reported to function as a neurotrophic factor, initiating neural differentiation and preventing neuronal cell death in vivo and in vitro. In this study, we examined the localization and synthesis of prosaposin in the rat cochlea. Intense prosaposin immunoreactivity was observed in the organ of Corti, stria vascularis, and spiral ganglion. In an immuno-electron microscopic study, prosaposin immunoreactivity was found mainly in lysosomal granules of the cells in these regions. In the lysosome, prosaposin does not always colocalize with cathepsin D, but was localized mainly in the dark area of the lysosome. Prosaposin mRNA was observed in these same regions. Our results suggest that prosaposin plays a role in homeostasis in the peripheral auditory system. 相似文献
47.
Akio Yano Osamu Igawa Masamitsu Adachi Junichiro Miake Yoshiaki Inoue Kazuyoshi Ogura Masaru Kato Kazuhiko Iitsuka Ichiro Hisatome 《Journal of interventional cardiac electrophysiology》2007,20(1-2):49-55
A 76-year-old man with two different sustained atrial arrhythmias that occurred after coronary artery bypass grafting underwent
electrophysiological studies. Macroreentrant atrial tachycardias were detected with an isolated slow pathway mimicking focal
activation on three-dimensional electroanatomical mapping. The slow conduction pathway in the right atrial free wall was assumed
to represent tissue damaged by right atrial cannulation during previous coronary artery bypass grafting. 相似文献
48.
Basilua Andre Muzembo Yasunori Nagano Masamitsu Eitoku Nlandu Roger Ngatu Tomomi Matsui Sabah Asif Bhatti Ryoji Hirota Kenji Ishida Narufumi Suganuma 《Environmental health and preventive medicine》2014,19(1):21-29
Objectives
The mechanism by which muscle weakness leads to an increased risk of death remains a subject of interest. In this context, the aim of this study is to assess the relationship between urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) and muscle strength, and other risk factors contributing to poor muscle strength in older persons.Methods
This was a cross-sectional study in which a total of 86 participants, both men and women, aged 65 years or above were screened for urinary 8-OHdG, and muscle strength as measured by handgrip strength.Results
Handgrip strength was lower in participants who had history of acute or chronic disease. Urinary 8-OHdG level was negatively associated with muscle strength, and the association remained after adjusting for confounding factors.Conclusions
Urinary 8-OHdG is associated with muscle strength. These findings may be clinically relevant as there is a possibility of controlling oxidative DNA damage by healthy behaviors related to lifestyle. 相似文献49.
Atsushi Hamabe Masamitsu Konno Nobuhiro Tanuma Hiroshi Shima Kenta Tsunekuni Koichi Kawamoto Naohiro Nishida Jun Koseki Koshi Mimori Noriko Gotoh Hirofumi Yamamoto Yuichiro Doki Masaki Mori Hideshi Ishii 《Proceedings of the National Academy of Sciences of the United States of America》2014,111(43):15526-15531
50.
Mahmoud Abdelghany Ibrahim Manabu Yasui Liton Kumar Saha Hiroyuki Sasanuma Masamitsu Honma Shunichi Takeda 《Environmental and molecular mutagenesis》2020,61(6):602-610
The OECD guidelines define the bioassays of identifying mutagenic chemicals, including the thymidine kinase (TK) assay, which specifically detects the mutations that inactivate the TK gene in the human TK6 lymphoid line. However, the sensitivity of this assay is limited because it detects mutations occurring only in the TK gene but not any other genes. Moreover, the limited sensitivity of the conventional TK assay is caused by the usage of DNA repair-proficient wild-type cells, which are capable of accurately repairing DNA damage induced by chemicals. Mutagenic chemicals produce a variety of DNA lesions, including base lesions, sugar damage, crosslinks, and strand breaks. Base damage causes point mutations and is repaired by the base excision repair (BER) and nucleotide excision repair (NER) pathways. To increase the sensitivity of TK assay, we simultaneously disrupted two genes encoding XRCC1, an important BER factor, and XPA, which is essential for NER, generating XRCC1 −/−/XPA −/− cells from TK6 cells. We measured the mutation frequency induced by four typical mutagenic agents, methyl methane sulfonate (MMS), cis-diamminedichloro-platinum(II) (cisplatin, CDDP), mitomycin-C (MMC), and cyclophosphamide (CP) by the conventional TK assay using wild-type TK6 cells and also by the TK assay using XRCC1 −/−/XPA −/− cells. The usage of XRCC1 −/−/XPA −/− cells increased the sensitivity of detecting the mutagenicity by 8.6 times for MMC, 8.5 times for CDDP, and 2.6 times for MMS in comparison with the conventional TK assay. In conclusion, the usage of XRCC1 −/−/XPA −/− cells will significantly improve TK assay. 相似文献