全文获取类型
收费全文 | 113篇 |
免费 | 4篇 |
专业分类
儿科学 | 3篇 |
妇产科学 | 1篇 |
基础医学 | 26篇 |
临床医学 | 11篇 |
内科学 | 23篇 |
皮肤病学 | 1篇 |
神经病学 | 8篇 |
特种医学 | 1篇 |
外科学 | 14篇 |
预防医学 | 13篇 |
药学 | 10篇 |
中国医学 | 1篇 |
肿瘤学 | 5篇 |
出版年
2022年 | 1篇 |
2021年 | 4篇 |
2020年 | 4篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 3篇 |
2014年 | 2篇 |
2013年 | 8篇 |
2012年 | 6篇 |
2011年 | 16篇 |
2010年 | 5篇 |
2009年 | 6篇 |
2008年 | 10篇 |
2007年 | 14篇 |
2006年 | 7篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1989年 | 1篇 |
排序方式: 共有117条查询结果,搜索用时 15 毫秒
41.
42.
43.
Xénia Mergui Marie-Line Puiffe Dominique Valteau-Couanet Marc Lipinski Jean Bénard Mounira Amor-Guéret 《BMC cancer》2010,10(1):473
Background
Human neuroblastoma (NB) cell lines may present with either one of the so-called S-and N-subtypes. We have previously reported a strong correlation between protein expression levels of vimentin, an S-subtype marker, and the p21Waf1 cyclin-dependent kinase inhibitor. We here investigated whether this correlation extend to the mRNA level in NB cell lines as well as in patients' tumors. We also further explored the relationship between expression of vimentin and p21, by asking whether vimentin could regulate p21 expression. 相似文献44.
Cécile Le Page Marie-Line Puiffe Liliane Meunier Magdalena Zietarska Manon de Ladurantaye Patricia N Tonin Diane Provencher Anne-Marie Mes-Masson 《Journal of ovarian research》2009,2(1):1-11
Background
Ovarian cancer is the most lethal gynecologic malignancy. The ovarian tumor microenvironment is comprised of tumor cells, surrounding stroma, and circulating lymphocytes, an important component of the immune response, in tumors. Previous reports have shown that the anti-apoptotic protein Bcl-2 is overexpressed in many solid neoplasms, including ovarian cancers, and contributes to neoplastic transformation and drug-resistant disease, resulting in poor clinical outcome. Likewise, studies indicate improved clinical outcome with increased presence of lymphocytes. Therefore, we sought to examine Bcl-2 expression in normal, benign, and cancerous ovarian tissues to determine the potential relationship between epithelial and stromal Bcl-2 expression in conjunction with the presence of lymphocytes for epithelial ovarian tumor progression.Methods
Ovarian tissue sections were classified as normal (n = 2), benign (n = 17) or cancerous (n = 28) and immunohistochemically stained for Bcl-2. Bcl-2 expression was assessed according to cellular localization, extent, and intensity of staining. The number of lymphocyte nests as well as the number of lymphocytes within these nests was counted.Results
While Bcl-2 staining remained cytoplasmic, both percent and intensity of epithelial and stromal Bcl-2 staining decreased with tumor progression. Further, the number of lymphocyte nests dramatically increased with tumor progression.Conclusion
The data suggest alterations in Bcl-2 expression and lymphocyte infiltration correlate with epithelial ovarian cancer progression. Consequently, Bcl-2 expression and lymphocyte status may be important for prognostic outcome or useful targets for therapeutic intervention. 相似文献45.
Bessaud M Jegouic S Joffret ML Barge C Balanant J Gouandjika-Vasilache I Delpeyroux F 《Journal of virological methods》2008,149(2):277-284
Human enteroviruses are among the most common viruses infecting humans and can cause diverse clinical syndromes ranging from minor febrile illness to severe and potentially fatal diseases. Biodiversity and evolution of human enterovirus genomes are shaped by frequent recombination events. Therefore, identification and characterization of circulating strains of enteroviruses require partial determination of different genomic regions. The development is described of a simple method allowing amplification and partial sequencing of the P1, P2 and P3 genomic regions of field human enterovirus strains isolated in cell cultures, by performing PCR on cDNAs generated through a single RT reaction. A set of generic primers were designed and tested on a panel of 90 field and prototype viruses belonging to the five species of human enteroviruses. This assay was shown to amplify efficiently the targeted regions of all the 90 genomes tested. The generated amplicons were sequenced successfully without the need for gel purification. This assay could be a valuable tool for laboratories interested in molecular epidemiology and evolution studies implicating a great number of human enterovirus strains isolated from human or environmental samples. 相似文献
46.
Michèle Kessler Carole Ayav Marie-Line Erpelding Cécile Couchoud 《Néphrologie & thérapeutique》2012,8(7):521-526
Changing trends concerning the incidence, sociodemographic features, and comorbidities observed among patients, starting their first renal replacement therapy by dialysis were studied in nine geographic regions in France participating in the REIN registry from 2004 to 2009. The standardized incidence of patients on dialysis remained stable while patient age tended to increase. After adjustment for age, the overall number of comorbid conditions remained unchanged, with a decrease in the number of patients with cardiovascular disease. These results, together with trends in laboratory results, suggest that the general health status of the patient population remains unchanged despite increasing age and that care for chronic renal failure has improved, generating an environment of prudent optimism for the future. 相似文献
47.
48.
49.
Alain Le Moine Véronique Flamand Aurore de Lavareille Frédéric Paulart Sofia Buonocore Marie-Line Vanderhaeghen Nathalie Nagy Claude Habran Robert Kiss Daniel Abramowicz Michel Goldman 《European journal of immunology》2002,32(1):174-181
A significant proportion of patients with the hypereosinophilic syndrome suffer from oligoclonal expansion of type 2 helper T lymphocytes (Th2). Herein, we first provide evidence that mice immunized at birth against a single MHC class II alloantigen develop pathological features mimicking this variant of the hypereosinophilic syndrome. Indeed, C57BL / 6 mice injected at birth with (C57BL/ 6 x bm12)F1 spleen cells displayed T lymphocytes producing high levels of IL-5 and IL-13, increased blood eosinophil counts, eosinophilic infiltrates in various tissues, hyperplasia of lymphoid tissues, as well as serum hyperIgE. Moreover, eotaxin mRNA accumulated in the spleen of these animals. IL-4-deficient mice developed neither expansion of Th2 cells nor pathological changes except splenomegaly. Eotaxin mRNA accumulation was also prevented in these animals. We conclude that neonatal exposure to a single MHC class II alloantigen is sufficient to elicit an IL-4-dependent hypereosinophilic syndrome mimicking the lymphocytic variant of this disorder in humans. 相似文献