EFFECT OF SUBCLINICAL LEAD INTOXICATION ON LARYNGEAL CANCER

SUMMARY In this study we investigated the possible relationship of laryngeal cancer and subclinical lead intoxication, using the depression of aminolevulinic acid dehydratase (ALAD) activity in blood as indicator. Twenty-six patients with laryngeal cancer and 53 normal controls met the criteria to enter the study. Blood ALAD activity values in the patients with laryngeal cancer ranged from 27.1 to 75.3 U/l with a mean of 50.79 U/l. The respective values in the control group ranged from 36.2 to 98 U/l with a mean of 59.76 U/l. There was a statistically significant difference between the two means (0.001 <p<0.01), whereas blood lead concentrations in all patients were within normal limits. These findings support the hypothesis that low level lead intoxication (subclinical blood lead levels), from cars, industries and products, may contribute to the risk of laryngeal cancer. Further investigation is needed to clarify the exact relationship between lead and cancer of the larynx.     

Experience with a transfusion recipient education program about hepatitis C

Shortly after test kits for antibodies to the hepatitis C virus (HCV) were licensed in May of 1990, our medical community undertook a public education program encouraging previous transfusion recipients to see their physicians about the wisdom of being tested for anti-HCV. In response, 1034 samples were received for testing. All samples repeatably reactive (RR) with anti-HCV enzyme-linked immunoassay (EIA) were tested further with a research recombinant immunoblot assay (RIBA). Overall, 76 of the 1034 (7.4%) recipient samples were RR and 64 of these (84.2%) were reactive with RIBA. Recipients transfused prior to surrogate testing (alanine aminotransferase [ALT] and anti-hepatitis B core [anti-HBc]) in 1986 showed a 8.6 percent reactivity with RIBA and those transfused after surrogate testing showed a 4.8 percent reactivity, a 44 percent reduction. Of the 57 recipient samples reactive with RIBA and suitable for assay, 11 (19.3%) had an elevated ALT. Among 76 randomly selected blood donors with RR EIAs studied for comparison with recipients, 20 (26.3%) were reactive with RIBA, 9 of which had an abnormal surrogate test that would have disqualified them. ALT concentrations were abnormal in 6 (30%) of the donors who were reactive on RIBA. We conclude that an education program that encourages previous transfusion recipients to seek medical advice about anti-HCV testing is practical from the standpoint of the blood center. We believe more widespread implementation of similar programs should be considered.(ABSTRACT TRUNCATED AT 250 WORDS)     

Fatal outcome in a patient with autoimmune hemolytic anemia associated with an IgM bithermic anti-ITP

BACKGROUND: Several cold autoantibodies (usually IgG) with IT specificity have been reported previously, as have autoantibodies with joint I and P blood group specificities (IP1, ITP1, iP1, IP). A fatal outcome associated with an IgM cold autoantibody of ITP specificity is reported. CASE REPORT: A 54-year-old man suffered from progressively severe cold autoimmune hemolytic anemia for 9 months. Hemoglobin concentration ranged from 6 to 7 g per dL (60-70 g/L) and reticulocytes from 3 to 5 percent (0.030-0.050). The direct antiglobulin test was weakly positive for IgM and strongly positive for C3d. The serum contained a cold agglutinin that reacted strongest with cord i red cells (RBCs) > adult I RBCs > adult i RBCs, which is consistent with IT specificity. The Donath-Landsteiner test was positive; the reaction was neutralized by globoside. The serum reacted weakly or was negative with RBCs from five group p blood donors, which suggests anti-ITP specificity. Dithiothreitol treatment of the serum abolished the cold agglutinin reactivity, which suggests that the anti-IT was IgM. The patient received > 40 RBC transfusions and failed to respond to oral steroids, oral cytoxan, high-dose pulse intravenous steroids, and plasma exchange at room temperature and at 35 degrees C. He died of sepsis following an unsuccessful trial of chlorambucil. Autopsy revealed unsuspected disseminated non-Hodgkin's lymphoma. CONCLUSION: Serologic studies are consistent with our patient's having a single IgM cold autoantibody with IT and P specificities (anti-ITP) and requiring both specificities on the same RBC to permit maximal antibody expression.     

Ni-Ti网状记忆合金支架治疗BPH远期疗效观察

目的：观察Ni—Ti网状记忆合金支架治疗BPH的远期疗效。方法：分析Ni—Ti网状记忆合金支架治疗BPH患者182例的临床资料,其中国产支架139例,进口支架43例。结果：182例患者中有94例远期效果良好,有效率达51．6％（其中国产组38．1％,进口组95．4％）。结论：Ni—Ti网状记忆合金支架治疗BPH远期疗效尚可,可以做为高危且预计生存期相对较长患者的一种安全可靠的治疗方法,进口支架效果尤佳。     

桔梗提取物中桔梗皂苷D的反相高效液相色谱检测方法研究

目的 建立桔梗提取物中桔梗皂苷D的高效液相色谱(HPLC)检测方法.方法 采用RP-HPLC法、蒸发光散射检测器(ELSD)测定桔梗皂苷D含量.色谱柱为Agilent ODS(4.6 mm×250 mm,5 μm),流动相为乙腈:0.5%乙酸溶液=28:72,流速1.0 ml/min;检测器为Waters ELSD 2420.结果 桔梗皂苷D在0.015 625～0.25 mg/ml范围内与峰面积具有良好的线性关系,r=0.999 6.桔梗皂苷D平均回收率为98.25%,RSD为1.18%.结论 通过6批不同的桔梗提取物的含量测定.证明该方法简便、准确、可靠.     

大鼠肝癌肿瘤转移相关基因的表达及不同中医治法作用

目的：观察肿瘤转移相关基因及转移抑制基因在DEN诱发大鼠肝癌组织中的表达及不同治法对其影响。方法：DEN诱发大鼠肝癌，经西药喃氟啶和不同中药治疗，取大鼠肝（含肝癌）组织抽提RNA，Affymetrix Rat 230A GeneChip检测基因表达的差异。结果：肝癌表达增加者55个，减少者8个，不同中西医治法有一定的调节作用。分析：肿瘤转移相关基因及转移抑制基因在肝癌中表达的改变和不同治法作用的差异，其具体调节途径和意义有待进一步研究。     

探讨糖调节受损与颈动脉中膜厚度的关系及意义

目的：探讨糖调节受损（IGR）患者颈动脉中膜厚度（IMT）增加的危险因素。方法：统计76例IGR患者及51例正常糖耐量（NGT）人的年龄、性别、体质量、血压、测空腹血糖、餐后2小时血浆血糖（OGTT）、空腹甘油三酯、总胆固醇（TC）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）,并计算体重指数,检测颈动脉中膜厚度（IMT）值。分析不同IGR患者IMT值的差异性。结果：IGT组及IFG＋IGT组IMT分别与IFG组及NGT组比较有显著差异（P〈0．05）,其他各组比较无显著差异。年龄、收缩压、TC、LDL—C、2hPG是IMT的危险因素。结论：与NGT组比较,IGT组、IFG＋IGT组患者早期已存在明显的动脉粥样硬化（AS）及与之相关的危险因素。     

归芍地黄汤合生脉散治疗围绝经期综合征73例临床观察

目的：观察归芍地黄汤合生脉散对围绝经期综合征的临床疗效。方法：73例围绝经期综合征患者中〈40岁12例,40~44岁14例,45~49岁22例,≥50岁17例。各年龄组均口服归芍地黄汤合生脉散加减进行治疗,分别与治疗前后及治疗12周后比较各年龄组库帕曼评分和激素水平的变化。结果：各年龄组经治疗后与本组治疗前比较库帕曼评分均有明显下降,差异有统计学意义（P〈0.01）。〈40岁的年龄组,促卵泡生成素（FSH）、雌二醇（E2）、促黄体生成（LH）均有改善,差异有统计学意义（P≤0.05）,40～44岁的年龄组,促卵泡生成素（FSH）有改善,差异有统计学意义（P〈0.05）,而雌二醇（E2）、促黄体生成（LH）均无改善（P〉0.05）。≥45岁的两个年龄组,促卵泡生成素（FSH）、雌二醇（E2）,促黄体生成（LH）均无改善（P〉0.05）。结论：归芍地黄汤合生脉散加减可以改善围绝经期综合征患者各年龄组的临床症状,有助于各年龄段的围绝经期妇女平稳度过围绝经期。     

西藏高原三种红景天离体枝条对昆明温度的响应研究

对采集自我国西藏自治区3个地区,分别属于3个不同种的红景天离体枝条在昆明的室温条件下进行新梢生长、根的发生及开花的观测,在昆明室温(室内散射光照;温度17~26℃)条件下,离体枝条均能够长出新梢,并很快开花,水培第7天新梢长度平均达到2.7 cm,2.3 cm和5.5 cm。水培5天后就喜玛红景天和齿叶红景天能观察到开花的新梢,至水培第7天有根生长的仅为长鞭红景天,其余两种红景天至第10天也未观察到生根现象。这些特性对于了解红景天的生物学特性,尤其是从营养生长到生殖生长的快速转化,以及为今后红景天的资源保护、人工繁殖、遗传生理、人工栽培研究有重要意义。     

酵母菌药用乳糖酶制备新方法

目的:建立一种简易的从酵母菌制备克鲁维酵母乳糖酶的方法.方法:在30L发酵罐中进行发酵,用对羟基苯甲酸酯进行细胞破壁,用超微过滤方法浓缩破壁液.结果:乳糖酶的收率可达到每毫升发酵液含11.4 ONPG单位.从酵终细胞中释放乳糖酶可达70%,每毫升牛奶中加入1.2 ONPG单位制备的液态乳糖酶可使牛奶中的乳糖水解率达到70%.