氮6-环戊基腺苷对大鼠肾缺血再灌注肾细胞凋亡的影响

目的观察氮6-环戊基腺苷(CPA)对大鼠缺血再灌注(I/R)肾细胞凋亡的影响。方法健康SD大鼠32只随机分成4组,每组8只。假手术组(S)组只进行手术操作,不做其他处理;I/R组肾缺血45 min再灌注24 h;C组给予CPA 1.0 mg/kg夹闭肾动脉前15 min腹腔注射,余操作同I/R组;CD组给予CPA 1.0 mg/kg和8-环戊基-1,3-二丙基黄嘌呤(DPCPX)1.0 mg/kg在夹闭肾蒂前15 min腹腔注射,余操作同I/R组。所有动物于再灌注24h处死。肾组织制备肾脏单细胞悬液,经碘化丙啶染色后用流式细胞分析法测定肾脏细胞凋亡率。结果 I/R组、C组和CD组肾组织细胞凋亡率明显高于S组(P均0.05),C组肾组织细胞凋亡率明显低于I/R组及CD组(P均0.05);I/R组与CD组比较差异无统计学意义(P0.05)。结论 CPA可抑制细胞凋亡,对大鼠肾I/R损伤有一定的保护作用。     

Early menarche is associated with insulin resistance in advanced maternal age before delivery

Abstract

Purpose: With the wide implementation of the universal two-child policy in China, the number of pregnant women in advanced maternal age (AMA) will increase gradually. We aimed to assess the association between age at menarche (AAM) and insulin resistance (IR) before delivery in AMA. Methods: A total of 80 pregnant women in AMA were consecutively enrolled before delivery in Zhongda hospital. Pregnant women were stratified into early menarche group and late menarche group according to the age of regular menstruation (about 13?years). At delivery, serum glucose and lipid levels were measured. IR was calculated by the method of homeostasis model assessment 2(HOMA2). Results: The fasting blood insulin (17.68(9.72–36.71) and 10.35(7.76–15.10), respectively; p?=?.006) and HOMA-IR (2.08(1.18–4.37) and 1.24(0.89–1.78), respectively; p?=?.005) were higher in early menarche group than in late menarche group. AAM was inversely associated with HOMA-IR in AMA (r=??0.27, p?=?.014). In the multivariable analysis, AAM in late menarche group was negatively related to the level of HOMA-IR compared to those in early menarche group (β=??2.275, p≤.0001). Conclusions: Taken together, our findings suggest that AAM was inversely associated with HOMA-IR in AMA. Furthermore, pregnant women in AMA with early menarche might have higher HOMA-IR levels than those with late menarche.

Clinical trial registration: Chinese Clinical Trial Registry (No. ChiCTR-RRC-16008714), retrospectively registered     

早期肠内免疫营养联合ERAS干预对腹腔镜胆囊切除术患者的影响

目的 探讨早期肠内免疫营养联合加速康复外科(ERAS)干预对腹腔镜胆囊切除术患者的影响.方法 选取我院收治的腹腔镜胆囊切除术患者107例,按照随机抽样法分成两组,对照组53例给予ERAS干预,观察组54例在此基础上增加早期肠内免疫营养干预,观察两组患者的术后消化运动功能、氧化应激指标、免疫球蛋白水平及并发症情况.结果 ...     

柴胡疏肝散在肝脏疾病中的应用研究进展

柴胡疏肝散出自《景岳全书》,由柴胡、川芎、陈皮、芍药、香附、炙甘草、炒枳壳7味中药组成,其主要功效为疏肝解郁、理气止痛,常用于治疗肝郁气滞引起的两胁肋部疼痛,胸闷善太息,情绪急躁易怒,反酸嗳气,脘腹胀满等。现代药理学研究发现,其组方主要包含皂苷类、黄酮类、酚酸类、萜类四种成分,具有抗炎、镇痛、护肝、抗肿瘤等多种药理作用,应用于各种肝脏疾病中可以有效改善患者症状、体征,改善肝功能、肝纤维化、血脂指标,提高患者生存质量。现从柴胡疏肝散近年来在肝纤维化、肝癌、肝硬化、脂肪肝、慢性胆囊炎等肝脏疾病中的相关应用,对柴胡疏肝散的最新研究动态进行综述,为临床应用柴胡疏肝散治疗肝脏疾病提供依据。     

大学生学业成就目标与考试焦虑的关系研究

目的探讨大学生学业成就目标倾向及其与考试焦虑之间的关系。方法采用考试焦虑自评量表和大学生学业成就目标倾向问卷对355名大学生进行随机抽样调查。结果①过程取向学业成就目标、结果取向学业成就目标、考试焦虑3个变量的年级主效应显著；②过程搴向的学业成就目标显著高于结果取向的学业成就目标；③考试焦虑与结果取向的学业成就目标呈极其显著正相关，与过程取向的学业成就目标呈极其显著负相关。结论学业成就目标对大学生考试焦虑具有显著影响。     

生物反馈电刺激联合盆底肌训练对产后盆底康复的疗效观察

目的探讨盆底肌训练联合生物反馈电刺激对产后盆底康复的疗效。方法选取165例初产妇(分成观察组和对照组)和59例经产妇(经产妇组),将初产妇随机分成观察组(85例)和对照组(80例)。观察组及经产妇组予以产后盆底肌训练联合功能性电刺激,对照组予以一般的产后健康宣教。产妇分别于产后3月随访,进行一般情况问卷调查及盆底肌肉功能评估、阴道张力测定,评价盆底肌肉训练的结果。结果观察组在盆底综合肌力、盆底脏器脱垂及尿失禁方面均较对照组及经产妇组有明显改善(P<0.05)。结论电刺激联合盆底肌训练对产后盆底康复是一个可行、安全、有效的方案,且对初产妇效果更明显。     

新型肿瘤标记物-循环miRNA在原发性肝癌中的研究现况

原发性肝癌(primary hepatic carcinoma,PHC)的发病率和死亡率居高不下,临床上亟需找到更可靠的生物标记物作为诊断及预后指标。目前研究发现循环miRNA的变化与癌的发生、发展具有密切的关系,同时部分循环miRNA可能作为肝癌的诊断和预后评估的分子标志物。本文就循环miRNA的发现、来源、功能、检测方法及在PHC中的关系做一综述。     

Tuning the performance of the non-fullerene organic solar cells by the polarizability

We report here the synthesis and characterizations of a novel series of acceptor copolymers with a broad absorption band. The acceptor polymers were synthesized as a copolymer of perylenediimide (PDI) and naphthalene imide (NDI) along with dithieno[3,2-b:2′,3′-d]silole (DTS) and N-alkyl dithieno[3,2-b:2′,3-d]pyrroles (DTP). When the dipole moment and polarizability of the acceptor polymer are compared, it is observed that when the dipole moment decreases, the polarizability becomes larger. The polarizability of polymers containing PDI is significantly greater than those containing NDI, and their polarizability change is in accordance with the change in the transient fluorescence lifetime. It was also found that the power conversion efficiency of the non-fullerene solar cell was strongly correlated to polarizability. The results demonstrate that the polarizability can be utilized to screen novel donor and acceptor polymers for the design and synthesis of high-performance solar cells.

The PCE of solar cells has a direct relationship with the polarizability is also directly related to the PL lifetime. The performance of non-fullerene solar cells can be controlled by polarizability.     

A study on graphene composites for peripheral nerve injury repair under electrical stimulation

Electrical stimulation (ES) provides an effective alternative to peripheral nerve repair via conductive scaffolds. The aim of the present study is to investigate a graphene (GR)/thermoplastic polyurethane (TPU) composite for the repair of peripheral nerve injury under ES. To this end, conductive composite membranes were fabricated by blending GR (2, 4 and 6 wt%) with TPU. GR maintains its own structure in the composite and enhances the mechanical and electrical properties of the composite. The composites with excellent biocompatibility had a hemolysis rate of less than 5%. As a result, the 4GR–TPU (4 wt% GR) sample with enhanced mechanical properties possessed the highest conductivity value of 33.45 ± 0.78 S m⁻¹. Compared with the non-conductive sample, 4GR–TPU was favorable for the viability of Schwann cells (SCs) under ES. When different voltages of ES were applied, a direct current of 10 mV was more suitable for the growth and proliferation of SCs. This study provides beneficial information for peripheral nerve repair via ES.

Electrical stimulation (ES) provides an effective alternative to peripheral nerve repair via conductive scaffolds.     

基于T7核酸外切酶的一步法克隆初探

目的 检测T7核酸外切酶是否可用于同源重组介导的DNA片段重组克隆技术.方法 利用PCR方法克隆pUC 19片段和卡那霉素供体片段后与T7核酸外切酶混合反应,将反应物直接转化大肠杆菌并克隆计数.结果 末端互补的线性化双链DNA分子与T7核酸外切酶反应后,无需纯化,即可直接用于转化大肠杆菌.和其他不依赖于连接酶的克隆法相似,T7核酸外切酶的一步克隆法(T7 exonuclease dependent one-step cloning,EDOC)的效率与末端互补序列的长度成正比,互补序列大于等于10 bp时即可稳定地产生克隆,互补区内的突变不利于同源重组发生.测序结果显示EDOC是一种精确克隆法,并未发现缺失,插入和点突变生成.结论 证实了T7核酸外切酶可被用于重组DNA的精确拼接,并成功建立了依赖于EDOC.