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91.
Makoto Bannai Katsushi Tokunaga Ling Lin Atsuko Ogawa Kiyoshi Fujisawa Takeo Juji 《Human immunology》1996,46(2):107-113
We developed a system for discriminating HLA-B40, B18, B27, and B37 alleles using a two-step PCR method followed by SSCP analysis. Fragments (0.8 kb) including exon 2, intron 2, and exon 3 were amplified in the first PCR. We used two sets of primers, one specific for HLA-B60-related alleles and the other specific for HLA-B6l-related, B18, B27, and B37 alleles. No amplifications of other class I genes or pseudogenes were observed. In the second PCR, exon 2 and exon 3 were amplified separately, using diluents of the first PCR products as templates. HLA-B6l-related, B18, B27, B37, and B60-related alleles were clearly discriminated in the SSCP analysis of the second PCR products. In a population study in which B6l alleles were analyzed, B*4003 was detected in two Japanese individuals in addition to two B6l alleles previously reported to occur in Japanese, B*4002 and B*4006. The relative frequencies of B*4002, B*4006, and B*4003 in Japanese were 58, 35, and 6%, respectively. The individuals having B*4003 are the first non-South Americans in whom this allele has been detected. The SSCP banding patterns of 18 HLA-B60-positive Japanese population samples were identical to those of a B*40012 sample for both exon 2 and exon 3. We also demonstrated that the B37 allele occurring in some Japanese is B*3701. 相似文献
92.
Maki-Paakkanen Jorma; Hayashi Makoto; Suzuki Takayoshi; Tanabe Hideyuki; Honma Masamitsu; Sofuni Toshio 《Mutagenesis》1995,10(6):513-516
The presence of centromeric DNA was studied in micronuclei isolatedfrom the blood of male ddY mic after five weekly intraperitonealinjections of mitomycu C (MMC), 1-ß-D-arabinofuranosylcytosine(Ara-C), colchi cine (COL) or vinblastine sulfate (VBL). Inagreement with our earlier findings, about half of the micronucleiisolate* from vehicle control mice showed centromere signalsa analyzed by fluorescence in situ hybridization (FISH) witha mouse major (gamma) satellite DNA probe. In an earlie experimentwith mice acutely exposed to the same chem icals, the clastogensMMC and Ara-C did not reduce thi proportion of micronuclei withcentromere signals. In the present study, however, MMC and Ara-Cdecreased the proportion of micronuclei with centromeres. Incontrast the spindle poisons COL and VBL increased the proportionof micronuclei that contained centromeres.
3To whom correspondence should be addressed 相似文献
93.
Hideo Sakuma Naoyoshi Mori Mizu Kojima Takeo Saito Fuminori Tachibana Makoto Naito 《Pathology international》1984,34(5):1157-1166
A case with lymphadenopathy of the left side of the neck in a 38-year-old male is described. He had a history of several relapses of about 10 years duration. Swollen lymph nodes were histologically similar to those of the hyaline-vascular type of Castleman's disease, but contained clear-cut lymph sinus and a sheet-like proliferation of plasma cells. Lymph follicles showed proliferation and atrophic germinal centers, in which cellular hypertrophy in the wall of ramifying small blood vessels, called angiosclerosis, was frequently encountered. During its progress, the patient developed plasmacytoma of the lymph nodes with varied clinical manifestations such as polyneuropathy, disturbance of gait, unusual perspiration, hirsuitism, gynecomastia, bilateral papilledema, and albumino-cytologic dissociation in cerebrospinal fluid. 相似文献
94.
Adjuvant Activity of 6-Amino-6-Deoxy-Muramyldipeptides and Their Acylamino Derivatives on the Induction of Delayed Hypersensitivity to Azobenzenearsonate-N-Acetyl-l-Tyrosine in Guinea Pigs 下载免费PDF全文
Ichiro Azuma Hiroyuki Okumura Ikuo Saiki Yoshiro Tanio Makoto Kiso Akira Hasegawa Yuichi Yamamura 《Infection and immunity》1981,32(3):1305-1308
The 6-amino-6-deoxy-N-acetylmuramyldipeptides and their 6-acylamino derivatives were shown to be active as adjuvants on the induction of delayed-type hypersensitivity to azobenzenearsonate-N-acetyl-l-tyrosine in guinea pigs. However, 6-acylamino-6-deoxy-N-(acyl)muramyldipeptides were inactive as adjuvants. 相似文献
95.
Assignment of a polymorphic locus of OS-4(D18S5) DNA segment to human chromosome region 18q21.3→qter
96.
Masamitsu Shirai Tatsuki Nagatsuka Makoto Tanaka 《Macromolecular chemistry and physics.》1978,179(1):173-179
The effect of polyanions on the formation of mixed dimers of methylene blue ( 1 ) and trypaflavine ( 2 ), methylene blue ( 1 ) and phenosafranine ( 3 ), and methylene blue ( 1 ) and pyronine G ( 4 ) was investigated spectrophotometrically. The following polyanions were used: poly(potassium styrenesulfonate) (PSS), poly(potassium vinyl sulfate) (PVS), and poly(sodium acrylate) (PAA). On addition of polyanions, the formation of mixed dimers was enhanced largely. Thermodynamic parameters inferred that the enhancement of the formation of mixed dimers in the presence of polyanions resulted from an entropic factor. 相似文献
97.
Masamitsu Shirai Tatsuki Nagatsuka Makoto Tanaka 《Macromolecular chemistry and physics.》1977,178(1):37-46
The structural effect of polyanions on the binding type of methylene blue ( 1 ) was investigated spectrophotometrically. 1 was bound to poly(potassium styrenesulfonate) (PSS) and poly(sodium 4-vinylphenylsulfate) (SVS) in the dimeric or slightly aggregated form and to poly(sodium vinylsulfonate) (SVF) and poly(potassium vinyl sulfate) (PVS) in the highly aggregated (polymeric) form. It was found that the flexibility of polyanions plays an important rǒle in the aggregation of bound 1 and that the difference between ? SO and ? OSO as binding site is not a significant factor. 相似文献
98.
Toshio Nishikawa Takeshi Kasajima Akira Kajita Mitsunori Yamakawa Gengo Mabuchi Hideo Orihata Toshimitsu Shibata Makoto Nakazawa 《Pathology international》1988,38(5):683-691
A case of adrenocortical carcinoma associated with congenital heart defect in a 6-month-old Japanese girl is reported. A fist-sized tumor was incidentally noted in the right hypochondrium upon admission for cardiac surgery. No clinical endocrinopathy was evident in this case. The resected tumor was encapsulated with smooth surface and no invasion to adjacent tissues or organs was observed. Histologically, the tumor was composed of small cells with granular or clear cytoplasm, and occasional giant cells with single or multiple nuclei. By electron microscopy, the tumor cells showed various nuclear contours with distinct nucleoli and had a moderate amount of cytoplasm containing abundant rough endoplasmic reticulum and mitochondria with variable-sized electron-dense granules. Intercellular desmosome-llke junctions were observed in some tumor cells. Immunohistochemlcally, the tumor cells contained granules positive for estriol, progesterone and Cortisol. These morphological findings including electron microscopic features suggested that the tumor cells had a malignant character. 相似文献
99.
Yoshiharu Ohaki Kazuaki Misugi Junya Fukuda Masahiko Okudaira Makoto Hieose 《Pathology international》1987,37(10):1581-1590
Three cases of pancreatoblastoma in children were examined immunohis-tochemically and the results were compared with those of pancreatic duct carcinoma in adults. The pancreatoblastoma demonstrated positive reactions to α-fetoprotein (AFP) (67%: 2/3), α-1-antitrypsin (AAT) (100%: 3/3), carcinoembryonic antigen (CEA) (67% : 2/3) and keratin (33% : 1/3), although CEA was only weakly positive in both cases. On the other hand, adult pancreatic duct carcinoma showed positive reactions as follows; AFP: 3% (1/29), AAT: 21% (6/29), CEA: 97% (28/29) and keratin: 93% (27/29). Also, endocrine substances including insulin, glucagon and somatostatin were all negative in the pancreatoblastomas. Two cases of pancreatoblastoma which were immunohistochemically positive for AFP also showed elevation of the serum AFP level clinically. The different expressive pattern of oncofetal antigens in pancreatoblastoma as compared with pancreatic duct carcinoma in adults may provide further supporting evidence for the embryonic nature of pancreatoblastoma, and suggests that such a pattern might be used as a tumor marker for pancreatoblastoma. ACTA PATHOL. JPN. 37 : 1581-1590, 1987. 相似文献
100.
Watanabe T Hasegawa G Yamamoto T Hatakeyama K Suematsu M Naito M 《Archives of histology and cytology》2003,66(2):155-162
Heme oxygenase (HO), the heme-degrading enzyme, plays an important role in heme catabolism. Among three isozymes, HO-1 is an inducible form expressed mainly in macrophages. In rat ontogeny, HO-1 immunoreactivity was detected in mononuclear cells in the yolk sac at 10 days of gestation. HO-1-expressing cells were then detected in the fetal liver and their numbers increased during the gestational period. The numbers of HO-1-positive cells and HO-1 mRNA levels in the liver peaked at 18 days of gestation. Most of the macrophages expressed both HO-1 and a macrophage scavenger receptor. Macrophages in the fetal liver showed marked hemophagocytosis. Macrophages in the lung, spleen, bone marrow, and other tissues also expressed HO-1. HO-1 immunoreactivity was also observed in syncytial cells of the chorionic villi, the endodermal layer of the yolk sac, and renal tubules of the fetus. Intestinal mucosal epithelial cells expressed HO-1 after birth. These findings imply that HO-1 is crucial for macrophages in heme catabolism from an early stage of ontogeny. HO-1 expression in non-macrophagic cells may be required for other purposes such as protection from oxidative stress and various stimuli. 相似文献