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81.
82.
Takaaki Konuma Shohei Mizuno Tadakazu Kondo Yasuyuki Arai Naoyuki Uchida Satoshi Takahashi Masatsugu Tanaka Takuro Kuriyama Shigesaburo Miyakoshi Makoto Onizuka Shuichi Ota Yasuhiro Sugio Yasushi Kouzai Toshiro Kawakita Hikaru Kobayashi Yukiyasu Ozawa Takafumi Kimura Tatsuo Ichinohe Yoshiko Atsuta Masamitsu Yanada for the Adult Acute Myeloid Leukemia Working Group of the Japanese Society for Transplantation Cellular Therapy 《Blood cancer journal》2022,12(5)
Unrelated cord blood transplantation (CBT) is an alternative curative option for adult patients with acute myeloid leukemia (AML) who need allogeneic hematopoietic cell transplantation (HCT) but lack an HLA-matched related or unrelated donor. However, large-scale data are lacking on CBT outcomes for unselected adult AML. To investigate the trends of survival and engraftment after CBT over the past 22 years, we retrospectively evaluated the data of patients with AML in Japan according to the time period of CBT (1998–2007 vs 2008–2013 vs 2014–2019). A total of 5504 patients who received single-unit CBT as first allogeneic HCT for AML were included. Overall survival (OS) at 2 years significantly improved over time. The improved OS among patients in ≥ complete remission (CR)3 and active disease at CBT was mainly due to a reduction of relapse-related mortality, whereas among patients in first or second CR at CBT, this was due mainly to a reduction of non-relapse mortality. The trends of neutrophil engraftment also improved over time. This experience demonstrated that the survival and engraftment rate after CBT for this group has improved over the past 22 years.Subject terms: Acute myeloid leukaemia, Cancer immunotherapy 相似文献
83.
Haruhisa Kato Makiko Naito Tomoko Saito Takuto Hideyama Yasuhiro Suzuki Takashi Kimura Shin Kwak Hitoshi Aizawa 《JOURNAL OF CLINICAL NEUROLOGY》2022,18(4):463
Background and PurposeAmyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease. Selective deficiency of edited adenosine deaminase acting on RNA 2 (ADAR2), a key molecule in the acquisition of Ca2+ resistance in motor neurons, has been reported in sporadic ALS (sALS) spinal motor neurons. Since ADAR2 activity is positively regulated by prolyl isomerase Protein never in mitosis gene A interacting-1 (Pin1), a known phosphorylation-dependent peptidyl-prolyl cis/trans isomerase, we investigated Pin1 expression in spinal motor neurons in sALS.MethodsSpecimens of the spinal cord were obtained from the lumbar region in eight sALS patients and age-matched five controls after postmortem examinations. The specimens were double stained with anti-Pin1 and anti-TAR DNA-binding protein of 43 kDa (TDP-43) antibodies, and examined under a fluorescence microscope.ResultsThis study analyzed 254 and 422 spinal motor neurons from 8 sALS patients and 5 control subjects, respectively. The frequency of motor neurons with high cytoplasmic Pin1 expression from the spinal cord did not differ significantly between sALS specimens without cytoplasmic TDP-43 inclusions and control specimens. However, in sALS specimens, neurons for which the Pin1 immunoluminescence intensity in the cytoplasm was at least twice that in the background were more common in specimens with cytoplasmic TDP-43 inclusions (p<0.05 in χ2 test).ConclusionsIn sALS, neurons with higher expression levels of Pin1 levels had more TDP-43 inclusions. Despite the feedback mechanism between Pin1 and ADAR2 being unclear, since Pin1 positively regulates ADAR2, our results suggest that higher Pin1 expression levels in motor neurons with TDP-43 pathology from sALS patients represent a compensatory mechanism. 相似文献
84.
85.
Masanao Yamashita Hiroshi Suemizu Norie Murayama Sayako Nishiyama Makiko Shimizu Hiroshi Yamazaki 《Regulatory toxicology and pharmacology : RTP》2014
To predict concentrations in humans of the herbicidal carbamate molinate, used exclusively in rice cultivation, a forward dosimetry approach was carried out using data from lowest-observed-adverse-effect-level doses orally administered to rats, wild type mice, and chimeric mice with humanized liver and from in vitro human and rodent experiments. Human liver microsomes preferentially mediated hydroxylation of molinate, but rat livers additionally produced molinate sulfoxide and an unidentified metabolite. Adjusted animal biomonitoring equivalents for molinate and its primary sulfoxide from animal studies were scaled to human biomonitoring equivalents using known species allometric scaling factors and human metabolic data with a simple physiologically based pharmacokinetic (PBPK) model. The slower disposition of molinate and accumulation of molinate sulfoxide in humans were estimated by modeling after single and multiple doses compared with elimination in rodents. The results from simplified PBPK modeling in combination with chimeric mice with humanized liver suggest that ratios of estimated parameters of molinate sulfoxide exposure in humans to those in rats were three times as many as general safety factor of 10 for species difference in toxicokinetics. Thus, careful regulatory decision is needed when evaluating the human risk resulting from exposure to low doses of molinate and related carbamates based on data obtained from rats. 相似文献
86.
Inhibition of autophagy by chloroquine makes chemotherapy in nasopharyngeal carcinoma more efficient
Tomomi Aga Kazuhira Endo Akira Tsuji Mitsuharu Aga Makiko Moriyama-Kita Takayoshi Ueno Yosuke Nakanishi Miyako Hatano Satoru Kondo Hisashi Sugimoto Naohiro Wakisaka Tomokazu Yoshizaki 《Auris, nasus, larynx》2019,46(3):443-450
Objectives
A combination of platinum-based chemotherapy and radiotherapy is the standard treatment for nasopharyngeal carcinoma (NPC). However, the efficacy of chemotherapy has reached a plateau. Many autophagy studies suggest that autophagy can either promote or suppress to cancer progression. Thus, a role of autophagy in the acquisition of chemoradioresistance has recently been a notable event. Therefore, we examined the relationship between autophagy and chemotherapy in NPC.Methods
The expression of Beclin 1 and microtubule-associated protein light chain 3 (LC3), a marker of autophagy, was determined by immunohistochemistry in the biopsy samples of patients with NPC before and after the first course of chemotherapy. Additionally, to investigate in the effect of autophagy suppression in chemotherapy, NPC cell line C666-1 cells were treated with cisplatin and/or chloroquine, an inhibitor of autophagy.Results
The expression of Beclin 1 increased after chemotherapy in all patients. In NPC cell line C666-1, compared to cisplatin alone, combination therapy (cisplatin and chloroquine) reduced cell viability, and promoted cell apoptosis.Conclusions
These results suggest that autophagy, represented by Beclin 1, is upregulated after chemotherapy in both in vitro and in vivo NPC studies. Inhibition of autophagy could therefore be new strategy for NPC treatment. 相似文献87.
Hirotomo Dochi Satoru Kondo Takayuki Murata Masaki Fukuyo Asuka Nanbo Kousho Wakae WenPing Jiang Toshihide HamabeHoriike Mariko Tanaka Takumi Nishiuchi Harue Mizokami Makiko MoriyamaKita Eiji Kobayashi Nobuyuki Hirai Takeshi Komori Takayoshi Ueno Yosuke Nakanishi Miyako Hatano Kazuhira Endo Hisashi Sugimoto Naohiro Wakisaka ShinHun Juang Masamichi Muramatsu Atsushi Kaneda Tomokazu Yoshizaki 《Cancer science》2022,113(8):2862
88.
Morimatsu Y Matsubara S Hirose N Ohkuchi A Izumi A Ozaki K Ozawa K Suzuki M 《Archives of gynecology and obstetrics》2008,277(3):267-270
Background Disseminated intravascular coagulation (DIC) caused by placental abruption usually improves rapidly after prompt delivery
and adequate anti-DIC treatment.
Case A 30-year-old nulliparous woman suffered from placental abruption at the 25th week of pregnancy, and emergent cesarean section
was done immediately. She exhibited DIC, which continued even after termination of the pregnancy and anti-DIC treatment. She
also showed neutropenia. We closely observed her, and at the 58th day postpartum, blast cells appeared in the peripheral blood
and she was diagnosed with acute promyelocytic leukemia (APL). Induction chemotherapy was done successfully. The close observation
after delivery enabled us to make the prompt diagnosis/treatment, leading to the complete remission.
Conclusion APL should be added to the list of differential diagnosis when DIC persists even after prompt delivery and appropriate anti-DIC
treatment after placental abruption. 相似文献
89.
90.
Kosuke Okuya Junki Mine Kaori Tokorozaki Isshu Kojima Mana Esaki Kohtaro Miyazawa Ryota Tsunekuni Saki Sakuma Asuka Kumagai Yoshihiro Takadate Yuto Kikutani Tsutomu Matsui Yuko Uchida Makoto Ozawa 《Emerging infectious diseases》2022,28(7):1451
Genetic analyses of highly pathogenic avian influenza H5 subtype viruses isolated from the Izumi Plain, Japan, revealed cocirculation of 2 genetic groups of clade 2.3.4.4b viruses among migratory waterfowl. Our findings demonstrate that both continuous surveillance and timely information sharing of avian influenza viruses are valuable for rapid risk assessment. 相似文献