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141.
Assessment of Becton Dickinson Plain and Serum Separator Tubes in Measurement of 25‐Hydroxyvitamin D3 (25OHD3) by HPLC and Immunoassay Methods
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142.
This study reports the viscosity (thickness) of nectar- and honey-thick liquids measured at a typical serving temperature.
Centipoise (cP) measurements were compared for three products (two starch and one gum-based thickener) mixed with five beverages
that set for three time intervals (manufacturer-recommended time to thicken, 10 min, and 30 min). The serving temperature
of the cold beverages was 4°C (water, apple juice, orange juice, and milk), and the hot beverage (coffee) was measured at
70°C. Statistical analysis showed that all factors interacted with one another, meaning that the viscosity of a nectar- or
honey-like liquid varies greatly depending on the type of thickening agent and beverage combination in relation to the amount
of time it thickens. Simply Thick, the gum-based thickener, typically produced samples that were the least viscous but they
maintained a more consistent level of thickness over time. Serving temperature results are contrasted with viscosity measurements
collected at room temperature, showing variable thickening patterns especially related to the type of thickening agent. 相似文献
143.
144.
Kifaya Azmi Abedelmajeed Nasereddin Suheir Ereqat Gabriele Schönian Ziad Abdeen 《Tropical medicine & international health : TM & IH》2010,15(8):872-880
The aim of the study was to assess the 7SL RNA PCR followed by restriction fragment length polymorphism (RFLP) and reverse dot blot (RDB) assays for use in identification of Old World Leishmania species. Species‐specific RFLP patterns were obtained for Leishmania major, Leishmania tropica and the Leishmania donovani complex when the 7SL RNA PCR product was digested with the restriction enzyme BsuRI, an isoschizomer of HaeIII. For the RDB assay, biotin‐labelled 7SL RNA amplicons were hybridized to Leishmania genus‐specific and species‐specific oligonucleotide probes immobilized onto a membrane. The Old World Leishmania species could be distinguished by using five probes: one that was a genus‐specific probe and hybridized to all Leishmania species (Lc), two that were specific for L. major (Lm1 and Lm2), one that was specific for L. tropica (Lt) and one that detected both L. major and L. tropica (Lmt). The PCR–RDB was 10 times more sensitive than 7SL PCR and can detect <1 parasite. In addition, the identification of species was easier and more reliable than with 7SL PCR–RFLP. 7SL PCR–RFLP detected parasites in 50 of 57 clinical samples, whereas PCR–RDB detected 53 and 55 were detected by amplification of kinetoplast (k) DNA. The 7SL RNA PCR has proven useful for direct diagnosis of Old World leishmaniasis, especially when combined with the RBD assay for species identification. 相似文献
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147.
Schepers E Glorieux G Dou L Cerini C Gayrard N Louvet L Maugard C Preus P Rodriguez-Ortiz M Argiles A Brunet P Cohen G Jankowski J Jankowski V Massy Z Rodriguez M Vanholder R;European Uremic Toxin Work Group 《Blood purification》2010,30(4):277-287
Chronic kidney disease is considered a major cause of cardiovascular risk and non-traditional risk factors remain largely unknown. The in vitro toxicity of 10 guanidino compounds (GCs) was evaluated via a standardized approach on different cell systems of relevance in cardiovascular disease. The parameters evaluated were production of reactive oxygen species, expression of surface molecules, cell proliferation, cytotoxicity and calcification. Several GCs had a stimulatory effect on monocytes and granulocytes (SDMA, creatine and guanidinobutyric acid (GBA)). Some GCs (guandine (G), guanidinosuccinic acid (GSA) and SDMA) inhibited endothelial cell proliferation or reduced calcification in osteoblast-like human VSMC (ADMA, GSA and SDMA). Stimulation of osteoclastogenesis could be demonstrated for ADMA, G, guanidinoacetic acid and GBA in a RAW264.7 cell line. No compounds were cytotoxic to AoSMC or endothelial cells, nor influenced their viability. GCs, especially SDMA, likely contribute to cardiovascular complications in uremia, mainly those related to microinflammation and leukocyte activation. 相似文献
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149.
Purpose
Sever hyponatremia is associated with a high mortality rate. It can occur during procedures requiring irrigation with a glycine solution.Clinical features
A 31-year old female patient underwent transcervical resection of a uterine myoma under general anesthesia. 35 minutes into surgery, a 41 min?1 bradycardia with hypertension (150/86 mmHg) was observed, followed by a 50 cmH2O increase in airway pressure and an expired CO2 of 49 mmHg, a sodium of 100 mmoles L?1 and a negative irrigation fluid water balance of 3000 mL. After administering 600 mL of 3% hypertonic saline, 60 mg of furosemide, performing a transvaginal puncture to release the extravased fluid in the peritoneal cavity, and artificial ventilation for 6 h, positive outcome was finally achieved.Conclusions
The resorption syndrome during transcervical resection of a uterine myoma is linked to the passage into the blood of the irrigation fluid that contains glycine. The symptoms are hidden during general anesthesia and severe hyponatremia can occur before the syndrome is detected. 相似文献150.
Ibrahim Fathi Ghalayini Mohammed A. Al-Ghazo Ziad Ali Elnasser 《International urology and nephrology》2009,41(4):805-813