The sense of personal ineffectiveness in patients with eating disorders: One construct or several?

The sense of personal ineffectiveness in eating disorders was investigated as a complex construct referring to various aspects of functioning rather than as the unidimensional trait assumed by prior studies. Eighteen patients and 18 controls were given a number of measures in order to test for ineffectiveness from several perspectives. The results indicated that patients with eating disorders experience a sense of ineffectiveness in many areas of their lives. Their more general attitudes about personal control appear to be dominated by their lack of control over their eating behaviour. However, these patients also exhibit distinct and specific difficulties in the areas of social effectiveness, personal independence, and self-esteem. These findings support the notion that ineffectiveness in eating disorders is not a single attitudinal style but a complex construct referring to a range of behaviors.     

Physiology of ageing

The impact that ageing has on organisms is a complex interaction between the processes of ageing at a cellular, organ and integrated systems level, and the effects of environmental factors such as nutrition, infection and trauma. Recovery from an insult that triggers a pathological response is never complete. The incremental fall in possible performance is part of the progressive diversity in ‘physiology’ that is the true hallmark of ageing. In this article we will outline some of the physiological changes, particularly cardiorespiratory, associated with the ageing process that will be of relevance to anaesthesia.     

Magnesium transport systems: genetics and protein structure (a review).

Magnesium is unique among biological cations. Its volume change from hydrated cation to atomic ion is over an order of magnitude larger than that of any other biological cation. This volume change presents particular problems for a magnesium transport system and suggests that these systems may be significantly different from other classes of ion transport systems. Detailed study of Mg2+ transport in complex organisms is limited by severe technical problems. However, molecular genetic techniques have enabled the isolation of three Mg2+ transport systems from the Gram-negative bacterium Salmonella typhimurium. The MgtA and MgtB transport systems are members of the P-type ATPase superfamily of transporters but possess unique characteristics among members of this family. The CorA transport protein is the first member of an entirely new class of transport proteins. In addition, another completely new family of Mg2+ transport proteins have been identified that is present in both Gram-negative and Gram-positive bacteria. Characterization of these transporters should provide substantial insight into Mg2+ transport and cellular Mg2+ homeostasis.     

加替沙星无菌检查方法的建立与标准操作探讨

目的:建立加替沙星原料及制剂无菌检查法及标准操作方法。方法:按2005年版中国药典无菌检查法验证实验的有关要求,通过接种阳性代表菌株,对薄膜过滤、添加中和剂等去除加替沙星抗菌活性的实验方法和条件进行验证,逐步建立加替沙星原料及制剂无菌检查的标准操作方法。结果:在对加替沙星不同原料及制剂样品适当的处理基础上,采用薄膜过滤法,以0.1%蛋白胨水溶液作为冲洗液,约每滤筒300 mL 的冲洗量,每筒培养基中加入0.1 mol·L~(-1)硫酸锰溶液3 mL 可去除加替沙星对细菌的抑菌作用。结论:加替沙星具有较强的抑菌活性,通过适当的样品处理、薄膜过滤法和添加硫酸锰溶液作为重金属络合剂,去除加替沙星抑菌活性,可对解决喹诺酮类抗生素无菌检查问题起到较好的参考作用。