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111.
Rabies is an important public health problem in South East Asia, with cases in this part of the world contributing to about
70% of the global burden. A large number of rabies cases occur in India, however, there is no organized system of surveillance
and hence there is a lack of reliable data. Moreover, comprehensive molecular epidemiological studies have not been performed
on Indian virus isolates. In this study, we determined the complete nucleotide and deduced amino acid sequence of a primary
isolate of rabies virus obtained from the brain of an infected patient. Comparison of the genomic sequence with those of the
ten fully sequenced rabies strains available in GenBank showed nucleotide homology ranging from 97% with AY956319 to 81% with
AY705373. Amino acid homology of nucleoprotein ranged from 99.7% with AY352493 to 92% with DQ875051. In case of the glycoprotein
gene, the homology ranged from 98.8% with AY956319 to 87.2 % with AY705373. An extensive nucleoprotein, glycoprotein, and
full-length genome-based phylogenetic analysis was performed along with sequences available from the GenBank. Phylogenetic
analysis of the complete genome sequence indicated that this isolate exhibited close homology with the ex Indian strain AY956319.
Primer sequences and the scheme of amplification can be availed from the authors on request. 相似文献