全文获取类型
收费全文 | 2584篇 |
免费 | 193篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 51篇 |
儿科学 | 64篇 |
妇产科学 | 82篇 |
基础医学 | 383篇 |
口腔科学 | 59篇 |
临床医学 | 292篇 |
内科学 | 405篇 |
皮肤病学 | 159篇 |
神经病学 | 206篇 |
特种医学 | 60篇 |
外国民族医学 | 1篇 |
外科学 | 254篇 |
综合类 | 24篇 |
一般理论 | 6篇 |
预防医学 | 291篇 |
眼科学 | 32篇 |
药学 | 157篇 |
中国医学 | 1篇 |
肿瘤学 | 257篇 |
出版年
2023年 | 33篇 |
2022年 | 50篇 |
2021年 | 101篇 |
2020年 | 74篇 |
2019年 | 82篇 |
2018年 | 97篇 |
2017年 | 62篇 |
2016年 | 84篇 |
2015年 | 58篇 |
2014年 | 86篇 |
2013年 | 150篇 |
2012年 | 162篇 |
2011年 | 147篇 |
2010年 | 98篇 |
2009年 | 101篇 |
2008年 | 146篇 |
2007年 | 126篇 |
2006年 | 150篇 |
2005年 | 148篇 |
2004年 | 110篇 |
2003年 | 122篇 |
2002年 | 95篇 |
2001年 | 25篇 |
2000年 | 16篇 |
1999年 | 29篇 |
1998年 | 33篇 |
1997年 | 33篇 |
1996年 | 16篇 |
1995年 | 19篇 |
1994年 | 26篇 |
1993年 | 18篇 |
1992年 | 26篇 |
1991年 | 20篇 |
1990年 | 15篇 |
1989年 | 13篇 |
1988年 | 13篇 |
1987年 | 16篇 |
1986年 | 15篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 15篇 |
1982年 | 20篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 12篇 |
1977年 | 7篇 |
1976年 | 8篇 |
1974年 | 10篇 |
1973年 | 7篇 |
排序方式: 共有2784条查询结果,搜索用时 15 毫秒
31.
Catharina Wising Jozef Azem Madeleine Zetterberg Liselott A Svensson Karin Ahlman Teresa Lagerg?rd 《Toxicon》2005,45(6):767-776
We investigated the impact of highly purified Haemophilus ducreyi cytolethal distending toxin (HdCDT) on the apoptosis and necrosis of various human cells; including myeloid cells, epithelial cells, keratinocytes, and primary fibroblasts. The levels of apoptosis and necrosis induced in these cells were compared to those induced by HdCDT in human T cells and in the Jurkat T cell line. Levels of caspase-3 activity were measured, and membrane changes like phosphatidylserine (PS) translocation was evaluated after double-staining with the fluorescein isothiocyanate (FITC)-labeled annexin V and propidium iodide (PI) using flow cytometry. HdCDT induced various degrees of apoptosis and necrosis in dose- and time-dependent manners in cells of various lineages. Early and late apoptosis (annexin V-stained cells) were induced in more than 90% of T cells and monocytes after treatment with 100 ng/ml HdCDT for 24 and 48 h, respectively. The corresponding numbers for epithelial cells, keratinocytes, and fibroblasts were 26-32% after treatment with 100 ng/ml HdCDT for 48 h. HdCDT appears to eliminate effectively by inducing apoptosis those cells that are involved in immune responses. Epithelial cells, keratinocytes and fibroblasts, which are important for the healing of chancroid ulcers, are eliminated by apoptosis or necrosis after contact with HdCDT, albeit slower and to a lesser extent than T cells. 相似文献
32.
33.
34.
35.
Varea Costello Madeleine Sowash Aahana Gaur Michael Cardis Helena Pasieka Glenn Wortmann Sheena Ramdeen 《Emerging infectious diseases》2022,28(5):1002
A case of monkeypox was diagnosed in a returning traveler from Nigeria to Maryland, USA. Prompt infection control measures led to no secondary cases in 40 exposed healthcare workers. Given the global health implications, public health systems should be aware of effective strategies to mitigate the potential spread of monkeypox. 相似文献
36.
Peter Gennemark Rasmus Jansson-Löfmark Gina Hyberg Maria Wigstrand Dorota Kakol-Palm Pernilla Håkansson Daniel Hovdal Peter Brodin Maria Fritsch-Fredin Madeleine Antonsson Karolina Ploj Johan Gabrielsson 《Journal of pharmacokinetics and pharmacodynamics》2013,40(6):651-667
Body composition and body mass are pivotal clinical endpoints in studies of welfare diseases. We present a combined effort of established and new mathematical models based on rigorous monitoring of energy intake (EI) and body mass in mice. Specifically, we parameterize a mechanistic turnover model based on the law of energy conservation coupled to a drug mechanism model. Key model variables are fat-free mass (FFM) and fat mass (FM), governed by EI and energy expenditure (EE). An empirical Forbes curve relating FFM to FM was derived experimentally for female C57BL/6 mice. The Forbes curve differs from a previously reported curve for male C57BL/6 mice, and we thoroughly analyse how the choice of Forbes curve impacts model predictions. The drug mechanism function acts on EI or EE, or both. Drug mechanism parameters (two to three parameters) and system parameters (up to six free parameters) could be estimated with good precision (coefficients of variation typically <20 % and not greater than 40 % in our analyses). Model simulations were done to predict the EE and FM change at different drug provocations in mice. In addition, we simulated body mass and FM changes at different drug provocations using a similar model for man. Surprisingly, model simulations indicate that an increase in EI (e.g. 10 %) was more efficient than an equal lowering of EI. Also, the relative change in body mass and FM is greater in man than in mouse at the same relative change in either EI or EE. We acknowledge that this assumes the same drug mechanism impact across the two species. A set of recommendations regarding the Forbes curve, vehicle control groups, dual action on EI and loss, and translational aspects are discussed. This quantitative approach significantly improves data interpretation, disease system understanding, safety assessment and translation across species. 相似文献
37.
38.
39.
M. Purokivi M.-R. Hirvonen J. Randell M. Roponen H. Tukiainen 《Inhalation toxicology》2013,25(12):1279-1290
Several epidemiological studies have revealed a large variety of adverse health effects related to exposure to microbes in moisture damaged buildings. Recently some evidence has been reported for a biochemical linkage between microbial exposure and the respiratory symptoms suffered by the occupants. The objective of the current study was to evaluate the value of nitric oxide (NO) measurements in determining the inflammatory status of airways in inhabitants of problem buildings. NO was measured by a chemiluminescence analyzer from the exhaled air. In addition, NO was determined as its metabolite nitrite from nasal lavage (NL) and induced sputum (IS) samples and also via the expression of inducible nitric oxide synthase (iNOS) in the cells. Occupants of moisture-damaged and reference schools were studied. The sampling was performed at the end of the spring term, at the end of the summer vacation, during the winter term, and after a 1-wk winter holiday. No statistically significant differences in NO levels were detected between the studied groups or between exposure and vacation periods. iNOS was not detected from IS or NL samples of the exposed occupants. These results suggest that NO measurements alone are not sufficient to quantify airway inflammation when evaluating subjects exposed to microbes present in moisture-damaged buildings. 相似文献
40.