大连口岸出国劳务人员疾病监测结果分析

[目的]了解出国劳务人员中传染病感染情况，为口岸疾病监测工作提供科学依据。[方法]对大连口岸13902名各类出国劳务人员按照《国际旅行健康检查证明书》逐项进行临床检查和实验室检验。[结果]检出各类疾病2742例，检出率为19．72％。其中传染性疾病548例，占病例总数的19．99％；非传染性疾病2194例，占病例总数的80．01％。[结论]出国劳务人员是国际旅行者中的特殊群体，由于长期的集体生活，极易患各种疾病，尤其是传染性疾病。应加强对出国劳务人员的传染病监测体检，并对劳务人员的健康状况进行评估，使他们了解自己的健康状况，防患于未然，提高自我保护意识，有效控制传染病在国内、国际间的传播。     

全自动血压计智能校准仪的研发

为了提高血压计校准的速度和准确性,本产品运用新型压力传感器,特殊的8955单片机调压控制程序,很好的解决了血压计(表)的校准问题.     

通络汤治疗糖尿病周围神经病变经验

糖尿病周围神经病变是糖尿病最常见的慢性并发症之一,其发病隐匿,可出现于糖尿病症状之前,易被忽视,严重者可致溃疡、坏疽,甚至截肢,是糖尿病致残的主要原因.本病中医多属于"痹症"、"痿症"范筹.     

接骨灵颗粒对家兔骨折愈合期的生物力学研究

目的:观察接骨灵颗粒促骨折愈合的作用.方法:对40只试验兔桡骨造成人工骨折模型,分为5组:对照组、接骨片、接骨灵(H)、接骨灵(M)、接骨灵(L),进行生物力学的拉伸、弯曲和冲击试验,并分别记录拉伸极限荷载、弯曲最大荷载和冲击值.结果:生物力学试验数据表明,接骨灵颗粒对兔桡骨骨折拉伸极限荷载、弯曲最大荷载、冲击值均显著高于对照组.结论:接骨灵颗粒具有良好的促骨折愈合作用.     

Interaction between the ACL graft and MCL in a combined ACL+MCL knee injury using a goat model

The optimal treatment for the MCL in the combined ACL and MCL-injured knee is still controversial. Therefore, we designed this study to examine the mechanical interaction between the ACL graft and the MCL in a goat model using a robotic/universal force-moment sensor testing system. The kinematics of intact, ACL-deficient, ACL-reconstructed, and ACL-reconstructed/ MCL-deficient knees, as well as the in situ forces in the ACL, ACL graft, and MCL were determined in response to two external loading conditions: 1) anterior tibial load of 67 N and 2) valgus moment of 5 N-m. With an anterior tibial load, anterior tibial translation in the ACL-deficient knee significantly increased from 2.0 and 2.2 mm to 15.7 and 18.1 mm at 30 degrees and 60 degrees of knee flexion, respectively. The in situ forces in the MCL also increased from 8 to 27 N at 60 degrees of knee flexion. ACL reconstruction reduced the anterior tibial translation to within 2 mm of the intact knee and significantly reduced the in situ force in the MCL to 17 N. However, in response to a valgus moment, the in situ forces in the ACL graft increased significantly by 34 N after transecting the MCL. These findings show that ACL deficiency can increase the in situ forces in the MCL while ACL reconstruction can reduce the in situ forces in the MCL in response to an anterior tibial load. On the other hand, the ACL graft is subjected to significantly higher in situ forces with MCL deficiency during an applied valgus moment. Therefore, the ACL-reconstructed knee with a combined ACL and MCL injury should be protected from high valgus moments during early healing to avoid excessive loading on the graft.     

维生素A干预对大鼠抗氧化能力及细胞膜流动性影响的研究

目的　通过对大鼠补充不同剂量维生素A(VA)观察机体抗氧化能力及对红细胞膜流动性的影响 ,了解抗氧化活性的最佳剂量。方法　将Wistar大鼠随机分为 4组 ,分别为维生素A缺乏组 (VA1组 )补充1 1 4 3(VA2组 )、4 2 86 (VA3组 )和 1 4 2 86 μgRE·kg- 1 ·d- 1 (VA4组 )维生素A三个剂量组。测定各组大鼠血浆VA含量、SOD、MDA和GSH Px的含量和活性 ,用荧光偏振度P值和微粘度 η值评价红细胞膜的流动性。 结果　各组血浆中VA水平随补充剂量的增加而增加。血浆中SOD、GSH -Px和MDA的结果显示 ,补充剂量为VA2、VA3组SOD的水平较VA缺乏组和VA4组明显偏低 (P <0 0 1 )。VA3组的MDA较其它 3个剂量组明显下降 (P <0 0 1 )。VA4组的SOD、MDA含量明显高于其它三个剂量组 (P <0 0 1 )。VA3组血浆中GSH -Px的活性明显升高 ,P <0 0 1。细胞膜流动性的结果显示 :VA3组P值最小 (P <0 0 1 ) ;VA4组P值明显增高 (P<0 0 1 )。微粘度 η在VA3组与VA缺乏组和VA2组之间未见明显差异 ,与VA4组相比明显下降 (P <0 0 1 )。结论　维生素A补充剂量为 4 2 86 μgRE·kg- 1 ·d- 1 时 ,能够发挥较好抗氧化作用 ,红细胞膜的流动性明显增加 ,过量摄入维生素A可造成机体的中毒反应 ,增加机体的氧化损伤     

β-紫罗兰酮对人乳腺癌细胞抑制作用

目的　研究 β 紫罗兰酮对人乳腺癌细胞 (MCF 7)生长的影响。 方法　采用细胞核分裂 ,生长曲线 ,集落形成和DNA合成实验 ,观察了用不同浓度 β 紫罗兰酮 (2 5、5 0、10 0和2 0 0 μmol L)对MCF 7细胞生长作用。 结果　β 紫罗兰酮可明显抑制MCF 7细胞增殖、细胞核分裂、集落形成和细胞DNA的合成 ,随着剂量的增加 ,抑制作用增强 ,IC50 值为 10 4 μmol L。细胞核分裂的抑制率分别为 2 4h 12 88%～ 6 8 6 7%。 4 8h 2 0 79%～ 87 79% ;集落形成抑制率分别为 2 4h 14 11%～ 6 5 18% ,4 8h 6 5 8%～ 72 4 8% ;DNA合成实验抑制率为 2 4h 16 75 %～6 5 33% ,4 8h 35 10 %～ 81 89%。结论　β 紫罗兰酮可抑制MCF 7细胞的生长 ,其机制需要进一步探讨。     

Comparison Study on Clinical and Neuropathological Characteristics of Hamsters Inoculated With Scraple Strain 263K in Different Challenging Pathways

OBJECTIVE: To understand the infectious characteristics of a hamster-adapted scrapie strain 263K with five different routes of infection including intracerebral (i.c.), intraperitoneal (i.p.), intragastrical (i.g.), intracardiac and intramuscular (i.m.) approaches. METHODS: Hamsters were infected with crude- or fine-prepared brain extracts. The neuropathological changes, PrP(Sc) deposits, and patterns of PK-resistant PrP were analyzed by HE stain, immnunohistochemistry (IHC) assay and Western blot. Reactive gliosis and neuron loss were evaluated by glial fibrillary acidic protein (GFAP) and neuron specific enolase (NSE) specific IHC. RESULTS: The animals inoculated in i.m. and i.p. ways with crude PrP(Sc) extracts showed clinical signs at the average incubation of 69.2 +/- 2.8 and 65.5 +/- 3.9 days. Inoculation in i.c. and intracardiac ways with fine PrP(Sc) extracts (0.00035 g) caused similar, but relative long incubation of around 90 days. Only one out of eight hamsters challenged in i.g. way with low dosage (0.01 g) became ill after a much longer incubation (185 d), while all animals (4/4) with high dosage (0.04 g) developed clinical signs 105 days postinfection. The most remarkable spongiform degeneration and PrP(Sc) deposits were found in brain stem among the five challenge groups generally. The number of GFAP-positive astrocytes increased distinctly in brain stems in all infection groups, while the number of NSE-positive cells decreased significantly in cerebrum, except i.c. group. The patterns of PK-resistant PrP in brains were basically identical among the five infection routes. CONCLUSION: Typical TSE could be induced in hamsters by inoculating strain 263K in the five infection ways. The incubation periods in bioassays depend on infective dosage, administrating pathway and preparation of PrP(Sc). The neuropathological changes and PrP(Sc) deposits seem to be related with regions and inoculating pathways.     

妊娠期接受MRI磁场照射对子代大鼠不同发育期MAPK的影响

【目的】　研究妊娠期接受磁共振 (magneticresonanceimaging ,MRI)复合磁场照射对子代大鼠不同发育期脑部丝裂原活化蛋白激酶 (mitogenactivatedproteinkinase ,MAPK) /细胞外信号调节蛋白激酶 (extracellularsignalregulatedproteinkinase ,ERK )表达的影响。 　【方法】　SD孕鼠妊娠第 12～ 18d给予 0 .3 5TMRI磁场照射 ,雌性子鼠1、2、5月龄时进行大脑切片免疫组化SP方法染色 ,并用图像分析仪进行半定量分析。　【结果】　MAPK/ERK免疫反应在大鼠脑切片上广泛表达 ;随着年龄增长 ,未发现其表达的变化 ;在各年龄段子鼠照射组和对照组间MAPK/ERK的免疫反应表达差异均无显著性 (P >0 .0 5 )。　【结论】　妊娠期MRI磁场照射后并未引起子代大鼠MAPK/ERK免疫反应表达的异常