全文获取类型
收费全文 | 9487篇 |
免费 | 704篇 |
国内免费 | 198篇 |
专业分类
耳鼻咽喉 | 119篇 |
儿科学 | 312篇 |
妇产科学 | 107篇 |
基础医学 | 841篇 |
口腔科学 | 165篇 |
临床医学 | 874篇 |
内科学 | 1524篇 |
皮肤病学 | 197篇 |
神经病学 | 410篇 |
特种医学 | 366篇 |
外国民族医学 | 1篇 |
外科学 | 1244篇 |
综合类 | 1158篇 |
现状与发展 | 2篇 |
一般理论 | 6篇 |
预防医学 | 849篇 |
眼科学 | 170篇 |
药学 | 1081篇 |
2篇 | |
中国医学 | 380篇 |
肿瘤学 | 581篇 |
出版年
2024年 | 13篇 |
2023年 | 84篇 |
2022年 | 274篇 |
2021年 | 386篇 |
2020年 | 232篇 |
2019年 | 219篇 |
2018年 | 246篇 |
2017年 | 194篇 |
2016年 | 229篇 |
2015年 | 286篇 |
2014年 | 378篇 |
2013年 | 457篇 |
2012年 | 751篇 |
2011年 | 778篇 |
2010年 | 593篇 |
2009年 | 528篇 |
2008年 | 618篇 |
2007年 | 598篇 |
2006年 | 576篇 |
2005年 | 465篇 |
2004年 | 380篇 |
2003年 | 311篇 |
2002年 | 253篇 |
2001年 | 205篇 |
2000年 | 197篇 |
1999年 | 127篇 |
1998年 | 54篇 |
1997年 | 48篇 |
1996年 | 48篇 |
1995年 | 45篇 |
1994年 | 47篇 |
1993年 | 43篇 |
1992年 | 78篇 |
1991年 | 69篇 |
1990年 | 52篇 |
1989年 | 45篇 |
1988年 | 54篇 |
1987年 | 40篇 |
1986年 | 36篇 |
1985年 | 52篇 |
1984年 | 47篇 |
1983年 | 25篇 |
1982年 | 17篇 |
1981年 | 19篇 |
1979年 | 21篇 |
1978年 | 11篇 |
1972年 | 16篇 |
1971年 | 21篇 |
1970年 | 12篇 |
1969年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
Naltrexone-3-salicylate (3), a prodrug of naltrexone (1), was prepared by a simple procedure from naltrexone-3-acetylsalicylate (2). The plasma (dog and human) hydrolysis half-life of 3 was found to be approximately 30 min. Compound 2 was previously shown to hydrolyze in dog and human plasma with a fast deacetylation step to 3, followed by slower hydrolysis of 3 to 1 (t
1/2, 30 min). Oral naltrexone bioavailability was greatly improved (30-fold) after oral administration of 3 to dogs, similar to the improvement observed after oral administration of 2. The half-life of naltrexone in dogs after oral administration of 3 was similar to that observed after oral administration of 2 (1 hr). 相似文献
62.
Zahir Ahmed 《Parasitology research》1959,19(1):67-99
Ohne ZusammenfassungMit 8 Textabbildungen in 21 Einzeldarstellungen 相似文献
63.
64.
Faiyaz-Ul-Haque M Ahmad W Zaidi SH Hussain S Haque S Ahmad M Cohn DH Tsui LC 《Clinical genetics》2004,66(2):144-151
Multiple hereditary exostoses (HME) is an autosomal dominant developmental disorder exhibiting multiple osteocartilaginous bone tumors that generally arise near the ends of growing long bones. Here, we report two large consanguineous families from Pakistan, who display the typical features of HME. Affected individuals also show a previously unreported feature--bilateral overriding of single toes. Analysis using microsatellite markers for each of the known EXT loci, EXT1, EXT2, and EXT3 showed linkage to EXT1. In the first family, mutation analysis of the EXT1 gene revealed that affected individuals were heterozygous for an in-frame G-to-C transversion at the conserved splice donor site in intron 1. This mutation is predicted to disrupt splicing of the first intron and produce a frameshift that leads to a premature termination codon. In the second family, an insertion of an A in exon 8 is predicted to produce a frameshift at codon 555 followed by a premature termination, a further 10 codons downstream. In both families, an increased number of affected male subjects were observed. In affected females in family 2, phenotypic variability and incomplete penetrance were noted. 相似文献
65.
Rapid exposure of cardiac muscle to high concentrations of caffeine releases Ca(2+) from the sarcoplasmic reticulum (SR). This Ca(2+) is then extruded from the cell by the Na(+)/Ca(2+) exchanger. Measurement of the current carried by the exchanger (I(Na/Ca)) can therefore be used to estimate of the Ca(2+) content of the SR. Previous studies have shown that caffeine, however, can also inhibit K(+) currents. We therefore investigated whether the inhibitory effects of caffeine on these currents could contaminate measurements of I(Na/Ca). Caffeine caused partial inhibition of the inward rectifier K(+) current (I(K1)): the outward current at -40 mV was 1.15+/-0.24 pA/pF in control and decreased to 0.34+/-0.15 pA/pF in the presence of 10 mmol/l caffeine (P<0.05, n=15). This was similar to the effect of caffeine on the holding current observed at -40 mV in the absence of K(+) channel block and could therefore account for the contaminating effects of caffeine observed during measurements of I(Na/Ca). Moreover, caffeine also partially inhibited the transient outward ( I(to)) and the delayed rectifier (I(K)) K(+) currents. 相似文献
66.
67.
Lymphocyte vaccination prevents spontaneous diabetes in the non-obese diabetic mouse. 总被引:2,自引:0,他引:2 下载免费PDF全文
The aim of this study was to investigate whether lymphocyte vaccination can prevent diabetes occurring in the non-obese diabetic (NOD) mouse, an animal model of human insulin-dependent diabetes mellitus (IDDM). The lymphocyte vaccine was composed of lymphocytes isolated from the spleens of diabetic NOD mice, activated in vitro using concanavalin A (Con A) and rendered immunogenic using glutaraldehyde treatment. These cells were used to vaccinate mice at 6 weeks with boosters at weeks 10, 14 and 18. The animals were then monitored for signs of diabetes until week 30. Twenty-eight NOD mice (11 male, 17 female) were T-lymphocyte vaccinated while 35 littermates (14 male, 21 female) were sham vaccinated with the vaccine carrier, as control mice. The percentage of mice remaining non-diabetic was 50% in the T-lymphocyte-vaccinated group compared with 20% in control mice (P < 0.05). When the results were divided according to sex of the mouse the percentage of female NOD mice remaining non-diabetic was 47.1% in the T-lymphocyte-vaccinated group compared to only 9.4% in the controls (P < 0.01), while in the males there was no significant difference between the groups. These results suggest that T-lymphocyte vaccination can prevent diabetes in NOD mice and that it has its greatest effect in females. The therapy is apparently safe and its efficacy indicates that it may be of value in prediabetes in man. 相似文献
68.
R. Blazev M. Hussain A.J. Bakker S.I. Head G.D. Lamb 《Journal of muscle research and cell motility》2001,22(3):277-286
This study investigated the effects of the protein kinase A (PKA) inhibitor, H-89, in mechanically-skinned muscle fibres and intact muscle fibres, in order to determine whether PKA phosphorylation is essential for normal excitation–contraction (E–C) coupling. In skinned EDL fibres of the rat, force responses to depolarization (by ion substitution) were inhibited only slightly by 10M H-89, a concentration more than sufficient to fully inhibit PKA. Staurosporine (1 M), a potent non-specific kinase inhibitor, also had little if any effect on depolarization-induced responses. At 1–2 M, H-89 significantly slowed the repriming rate in rat skinned fibres, most likely due to it deleteriously affecting the T-system potential. With 100 M H-89, the force response to depolarization by ion substitution was completely abolished. This inhibitory effect was reversed by washout of H-89 and was not due to block of the Ca2+ release channel in the sarcoplasmic reticulum (SR). In intact single fibres of the flexor digitorum longus (FDB) muscle of the mouse, 1–3 M H-89 had no noticeable effect on action-potential-mediated Ca2+ transients. Higher concentrations (4–10 M) caused Ca2+ transient failure in fibres stimulated at 20 Hz in a manner indicative of action-potential failure. At 10–100 M, H-89 also inhibited net Ca2+ uptake by the SR and affected the Ca2+-sensitivity of the contractile apparatus in rat skinned fibres. All such effects were proportionately greater in toad muscle fibres. These results do not support the hypothesis that phosphorylation is essential for the Ca2+ release channel to open in response to voltage-sensor activation in skeletal muscle fibres. 相似文献
69.
In anesthetized, artificially ventilated guinea pigs, intranasal and intravenous administration of albuterol produced the same maximum degree of protection against bronchoconstriction induced by bilateral electrical stimulation of the cervical vagal nerves. Intranasal albuterol showed a slower onset of action than intravenous albuterol and exhibited equivalent cardiovascular side effects for the same level of bronchoprotection. Accordingly, intranasal albuterol may represent an alternative to metered-dose inhalation for prophylaxis and treatment of bronchoconstriction in humans. 相似文献
70.
目的研究1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者CYP17A1基因突变特点,并结合患者的临床表现与基因突变类型初步探讨P450C17酶蛋白的结构与功能的关系。方法收集1例17α-羟化酶/17,20-裂解酶部分性联合缺陷症患者的临床资料及其亲属血标本,提取基因组DNA,设计7对引物扩增CYP17A1基因的8个外显子及外显子与内含子的连接区域,琼脂糖凝胶电泳鉴定PCR产物,产物胶回收后直接做为DNA双链模板测序。DNA双链模板不一致的PCR产物经克隆后测序。测序结果在核苷酸序列数据库进行比较分析。结果患者CYP17A1基因突变检测结果为5994-5995delAT/7541C>T复合杂合子。这两种突变均未见报道。推测5994-5995delAT导致I259H,274X,突变形成的截短蛋白质缺少血红素结合区域,因此是没有功能的;而通过人类P450C17酶计算机模型分析显示7541C>T导致的A398V远离酶的活性中心,推测突变可能使酶的活性减弱,而不是完全地丧失。患者临床表现为有自发不规则月经及轻度高血压、低血钾,结合激素测定结果提示肾上腺和性腺保留部分功能。因而患者的基因型与其临床表型是一致的。结论应进行突变P450C17酶的功能学研究来进一步明确结构改变对功能的影响。 相似文献