Design and Structure–Activity Relationship of a Potent Furin Inhibitor Derived from Influenza Hemagglutinin

Furin plays an important role in various pathological states, especially in bacterial and viral infections. A detailed understanding of the structural requirements for inhibitors targeting this enzyme is crucial to develop new therapeutic strategies in infectious diseases, including an urgent unmet need for SARS-CoV-2 infection. Previously, we have identified a potent furin inhibitor, peptide Ac-RARRRKKRT-NH₂ (CF1), based on the highly pathogenic avian influenza hemagglutinin. The goal of this study was to determine how its N-terminal part (the P8–P5 positions) affects its activity profile. To do so, the positional-scanning libraries of individual peptides modified at the selected positions with natural amino acids were generated. Subsequently, the best substitutions were combined together and/or replaced by unnatural residues to expand our investigations. The results reveal that the affinity of CF1 can be improved (2–2.5-fold) by substituting its P5 position with the small hydrophobic residues (Ile or Val) or a basic Lys.     

Sexual behaviour and condom use among unmarried young men in Cambodia

The objective of this paper is to describe the sexual behaviour of 665 unmarried men aged 15-24, and explore factors associated with condom use at last intercourse. We use data from a cross-sectional survey conducted in economically marginal areas of Phnom Penh and Kratie town, Cambodia. One-third of respondents reported intercourse and half had had sex by the time they were 23. Of these, 39% had given money or gifts in exchange for sex. Transactional sex often occurred in the company of other males, and condom use was higher among those males compared with those who were alone. Of all sexually active respondents, half reported three or more partners, and 71% used a condom at last sex. Regression results showed that condom use varied by type of partner, was less likely among males outside the education system, and higher among those more positive and informed about condoms. Our findings highlight the need for HIV prevention efforts to encourage young men to use condoms with all intimate partners, promote advantages of condoms for both disease and pregnancy prevention, and address the needs of young men no longer in education. Further research, to understand the positive influence that peers have on condom use in certain circumstances, would be useful.     

Psychoeducational Intervention for Symptom Management of Fatigue,Pain, and Sleep Disturbance Cluster Among Cancer Patients: A Pilot Quasi-Experimental Study

Objectives

To assess the feasibility of conducting a trial of a psychoeducational intervention involving the provision of tailored information and coaching to improve management of a cancer-related symptom cluster (fatigue, pain, and sleep disturbance) and reduce symptom cluster impacts on patient health outcomes in the Vietnamese context and to undertake a preliminary evaluation of the intervention.

Psychotropic drug use in non-psychiatric departments of three European university hospitals

The use of psychotropic drugs in the university hospitals in Tartu, Estonia; Huddinge, Sweden; and Badajoz, Spain, were studied, using the defined daily doses per 100 bed-days (DDD/100 bed-days) method. The total amount of drugs used in the surgical and medical departments was 50 DDD/100 bed-days in Huddinge, versus 33 and 14 DDD/100 bed-days in Tartu and Badajoz, respectively. Barbiturates accounted for 35% of all psychotropics in Tartu but were practically not used in Huddinge. In contrast, antidepressants were practically not used in Tartu. The use of psychotropic drugs in the intensive care units was highest in Huddinge (320 DDD/100 bed-days), compared with Tartu and Badajoz (177 and 96 DDD/100 bed-days, respectively). The frequency of psychotropic drug use were strikingly different in the three hospitals studied.