Th17 cell‐derived miR‐155‐5p modulates interleukin‐17 and suppressor of cytokines signaling 1 expression during the progression of systemic sclerosis

Background miR‐155‐5p is associated with autoimmune diseases. T helper 17 (Th17) cells, interleukin (IL)‐17, and suppressor of cytokines signaling 1 (SOCS1) have important roles in the pathogenesis of systemic sclerosis (SSc). The purpose of this study was to explore the role of miR‐155‐5p in the regulation of IL‐17 and SOCS1 expression in Th17 cells and the subsequent effect on SSc disease progression.MethodsTh17 cells were isolated from peripheral blood mononuclear cells of SSc patients and healthy controls (HCs). RT‐qPCR and western blotting were used to examine the expression patterns of miR‐155‐5p, IL‐17, and SOCS1. Luciferase reporter assays were performed to confirm SOCS1 as a target of miR‐155‐5p. RNA pull‐down assays were performed to detect the interaction of IL‐17 and SOCS1 with miR‐155‐5p. In situ hybridization was performed to analyze the co‐expression pattern of miR‐155‐5p and IL17A in Th17 cells.ResultsThe levels of Th17 cell‐derived miR‐155‐5p were significantly up‐regulated in SSc patients compared with HCs, and its levels were negatively correlated with SOCS1 levels. Meanwhile, miR‐155‐5p positively regulated IL‐17 expression levels in Th17 cells isolated from SSc patients as the disease progressed. Using pmirGLO vectors, SOCS1 was confirmed as a target of miR‐155‐5p. The binding status of IL‐17 and SOCS1 to miR‐155‐5p was related to SSc progression. An increase in the co‐localization of miR‐155‐5p and IL‐17 was associated with greater SSc progression.ConclusionsIL‐17 and SOCS1 expression modulated by Th17 cell‐derived miR‐155‐5p are critical for SSc progression, which may provide novel insights into the pathogenesis of SSc.     

170例肝移植病例医疗质量费用分析

目的:分析肝移植病例住院费用构成并评价其医疗质量.方法:收集手术操作代码为50.5001的病案170份,采用<病例分型质量费用管理系统>软件和SAS6.1 2,进行描述性统计分析.结果与讨论:肝移植治疗水平不断提高,但费用十分昂贵,必须采取各种有效措施,降低患者负担.     

脂蛋白脂酶基因PvuⅡ位点多态性与高脂血症人群膳食干预易感性的关系

为了解脂蛋白脂酶 (LPL)基因PvuⅡ位点多态性与高脂血症人群膳食干预效果的关系 ,从北京市西城区 8个社区居民中筛出 43 6名高脂血症患者 ,分为干预组 (2 48人 )和对照组 (188人 ) ;对两组人群进行血脂谱水平检测、膳食调查、体格检查及LPL基因PvuⅡ位点多态性检测 (PCR RFLP方法 ) ,并对干预组进行为期 6个月的膳食干预。结果表明与对照组相比 ,干预组膳食总能量、脂肪供能比及胆固醇摄入量明显降低 ,血清TC、LDL C和HDL C水平明显下降 (P <0 0 5 ) ,且LPL PvuⅡ位点 (+ +)基因型携带者TC和LDL C水平降低幅度大于 (+ - )及 (- - )基因型 ;多元线性回归分析显示膳食干预易感的因素包括LPL(+)等位基因、基限TC、LDL C水平较高以及超重等。本研究可初步得出LPL基因PvuⅡ位点 (+)等位基因与高脂血症人群膳食干预易感性密切相关 ,但限于研究对象的代表性该结论还需要进一步研究支持     

台州市2018/2019年度社区老年人接种流感疫苗的保护效果

目的评估台州市2018/2019年度社区老年人接种三价灭活流感疫苗(TIV)的保护效果(VE)。方法运用前瞻性队列研究设计,招募接种和未接种TIV的≥60岁社区老年人随访6个月,观察流感样疾病(ILI)、因ILI就诊、因ILI或肺炎住院、因呼吸或循环系统疾病住院4种临床结局,计算TIV的VE。结果 TIV接种组、未接种组分别纳入研究对象1 048名、1 025名。接种TIV后1-3月预防4种临床结局的VE(95%CI)分别为-25.1(-80.4-13.2)%、-33.1(-99.2-11.1)%、35.8(-124.9-81.7)%和-12.6(-229.2-61.5)%;接种TIV后4-6月分别为25.5(-7.5-48.4)%、35.1(3.0-56.5)%、1.4(-249.1-72.1)%和-11.5(-240.9-63.5)%;接种TIV后1-6月分别为5.9(-24.2-28.7)%、11.6(-18.9-34.3)%、21.7(-99.2-69.2)%和-13.9(-155.5-49.2)%。结论台州市2018/2019年度社区老年人接种TIV对预防ILI病例发病、就诊和相关住院具有一定的保护效果。     

骨折相关感染治疗的研究进展

骨折相关感染(FRI)是骨科常见的手术并发症,FRI发生的多样性以及近年来对抗生素的滥用给FRI的诊断和治疗带来了一定难度.术前体格检查、影像学检查及相关的实验室检查对明确诊断具有重要意义.治疗方法主要以静脉应用抗生素、局部清创、术中植入抗生素载体为主,目前临床应用效果最好且满意度较高的是术中植入抗生素载体,其具有较好...     

Early postoperative pain after subxiphoid uniportal thoracoscopic major lung resection: a prospective,single- blinded,randomized controlled trial

Open in a separate window OBJECTIVESPreoperative selection bias led to the inability to generalize the proposed benefit of subxiphoid uniportal video-assisted thoracoscopic surgery (SVATS) as having less postoperative pain than uniportal intercostal VATS. So, we conducted this prospective, single-blinded, randomized controlled trial to investigate the hypothesis that SVATS may have less early postoperative pain than UVATS in patients who undergo major lung resection for early-stage lung cancer.METHODSA total of262 patients were randomly allocated between 2 groups (each with 131 patients), the first being the UVATS group and the second being the SVATS group. The values indicated on the numerical rating scale (NRS) of pain were collected at 24 h and 48 h during rest and during coughing. In addition, different perioperative variables were analysed and compared between the 2 groups.RESULTSMultiple linear regression analysis showed that the type of surgical approach was a significant predictor of the postoperative NRS values. The postoperative NRS pain values were significantly lower in the SVATS group after 24 h during rest and coughing and after 48 h during coughing. Postoperatively, patients in the SVATS group got out of bed significantly earlier [16.37 (2.54) vs 18.05 (3.29) h, p < 0.001]. The SVATS group showed a significantly higher rate of intraoperative arrhythmia [20 (15.3%) vs 3 (2.3%) patients, p = 0.03].CONCLUSIONSSVATS major pulmonary resection in early-stage lung cancer is associated with less early postoperative pain than the UVATS approach. Operating on patients with cardiac problems using the SVATS approach is still a limiting factor for randomization due to the potential compression on the heart with resulting arrhythmia.Clinical trial registrationThe trial was registered under clinical trials.gov Identifier: {"type":"clinical-trial","attrs":{"text":"NCT03331588","term_id":"NCT03331588"}}NCT03331588. https://clinicaltrials.gov/ct2/show/{"type":"clinical-trial","attrs":{"text":"NCT03331588","term_id":"NCT03331588"}}NCT03331588.     

Targeted peptide-modified oxidized mesoporous carbon nanospheres for chemo-thermo combined therapy of ovarian cancer in vitro

Ovarian cancer remains one of serious hazards to human health due to many drawbacks of existing available treatment options. In this study, a multifunctional chemo-thermo combined therapy nanoplatform (OMCNPID) was successfully prepared, which is composed of I₆P₈ peptide as a targeting moiety to interleukin-6 receptors (IL-6Rs), oxidized mesoporous carbon nanospheres (OMCN) as a near infrared (NIR)-triggered drug carrier and doxorubicin (DOX) as a chemotherapeutic drug and fluorescent agent. The synthesized multifunctional nanoplatform displayed high storage capacity for drugs and excellent photothermal properties. Besides, DOX was rapidly released from OMCNPID at the condition of low pH and NIR laser irradiation due to the dissociation of DOX from graphitic cores of OMCN. In vitro experimental results verified that OMCNPID could be markedly taken up by SKOV-3 monolayer cells and tumor spheroids, and revealed a remarkable synergistic chemo-photothermal effect against ovarian cancer. All the results demonstrated that OMCNPID is a pH/NIR dual-stimulus responsive nanoplatform and can achieve efficient chemo-thermo combined therapy.     

基于项目反应理论的中国职业人群职业紧张测量核心量表条目分析

目的 利用项目反应理论（item response theory,IRT）对《中国版职业紧张核心量表》质量进行分析与评价,为后期量表使用和修订提供参考依据。方法 采用方便抽样方法,抽取湖北省两家三甲医院和多家一、二级医院共1261名医务人员作为研究对象,应用《中国版职业紧张核心量表》调查其职业紧张情况。采用主成分分析验证量表4个维度的单维性。采用IRT中的Same Jima等级反应模型计算每个条目的区分度、难度系数和信息量,从微观角度评价量表的测量特性。结果 量表4个维度均满足单维性假设。IRT结果显示所有条目的区分度较好,取值范围在0.67~3.10;17个条目中有13个条目的难度系数在-2.78~2.30之间,且不存在难度逆反现象,条目9和11难度过高且难度逆反,条目15和16难度过低过高并存且有难度逆反现象,提示待改进;除了条目9、11和15提供的信息量中等,条目16和17提供的信息量较差以外,其余条目的信息量均较好。结论 《中国版职业紧张核心量表》所有条目的区分度较好。从难度系数和信息量两个角度,条目9、11、15、16、17的测验质量均是有待改进的,其余条目性能良好,建议针对上述分析结果结合专家意见对问题条目进行修订。     

参蛤河车胶囊治疗慢性阻塞性肺疾病160例

目的:观察补气健脾、益肾活血类中药配伍治疗高寒地区慢性阻塞性肺疾病的疗效。方法:采用参蛤河车胶囊(西洋参、蛤蚧、紫河车、桑白皮、杏仁、川贝母、茯苓、桃仁等)治疗本病160例。结果:总有效率达76.8%。提示:本方具有补肺健脾,益肾活血的功效。     

LOX与VEGF蛋白在人喉鳞癌中的表达及其相关性研究

目的检测赖氨酰氧化酶（LOX）、血管内皮生长因子（VEGF）蛋白在人喉鳞癌中的表达,探讨其对喉鳞癌转移及预后的影响及意义。方法采用免疫组织化学方法检测14例有淋巴结转移的喉鳞癌及24例未见淋巴结转移的原位癌组织中LOX及VEGF的表达情况,并分析其表达的相关性。结果①LOX、VEGF在喉鳞癌组织中表达显著高于癌旁正常组织（P均〈0．05）。②VEGF在淋巴结转移组与无转移组表达阳性率分别为100％和71％,具有显著性差异（P〈0．05）;LOX在淋巴结转移组与无转移组表达无显著性差异（P〉0．05）。③LOX、VEGF的表达与患者的年龄、性别、临床分型及临床分期等临床病理因素无关。④LOX与VEGF在人喉鳞癌组织中表达呈正相关。结论LOX与VEGF存在正相关性,可作为喉癌治疗的一个新靶点。VEGF的高表达可能是造成LOX表达变化的重要影响因素之一。