全文获取类型
收费全文 | 15084篇 |
免费 | 772篇 |
国内免费 | 124篇 |
专业分类
耳鼻咽喉 | 98篇 |
儿科学 | 335篇 |
妇产科学 | 409篇 |
基础医学 | 1937篇 |
口腔科学 | 362篇 |
临床医学 | 1049篇 |
内科学 | 4252篇 |
皮肤病学 | 222篇 |
神经病学 | 1500篇 |
特种医学 | 610篇 |
外科学 | 2233篇 |
综合类 | 42篇 |
一般理论 | 1篇 |
预防医学 | 591篇 |
眼科学 | 162篇 |
药学 | 766篇 |
中国医学 | 23篇 |
肿瘤学 | 1388篇 |
出版年
2024年 | 13篇 |
2023年 | 98篇 |
2022年 | 210篇 |
2021年 | 434篇 |
2020年 | 228篇 |
2019年 | 318篇 |
2018年 | 413篇 |
2017年 | 307篇 |
2016年 | 343篇 |
2015年 | 378篇 |
2014年 | 594篇 |
2013年 | 729篇 |
2012年 | 1016篇 |
2011年 | 1039篇 |
2010年 | 678篇 |
2009年 | 616篇 |
2008年 | 1064篇 |
2007年 | 1049篇 |
2006年 | 941篇 |
2005年 | 1000篇 |
2004年 | 885篇 |
2003年 | 819篇 |
2002年 | 725篇 |
2001年 | 118篇 |
2000年 | 109篇 |
1999年 | 133篇 |
1998年 | 157篇 |
1997年 | 125篇 |
1996年 | 138篇 |
1995年 | 101篇 |
1994年 | 109篇 |
1993年 | 86篇 |
1992年 | 100篇 |
1991年 | 91篇 |
1990年 | 86篇 |
1989年 | 63篇 |
1988年 | 64篇 |
1987年 | 48篇 |
1986年 | 58篇 |
1985年 | 42篇 |
1984年 | 49篇 |
1983年 | 30篇 |
1982年 | 42篇 |
1981年 | 38篇 |
1980年 | 48篇 |
1979年 | 38篇 |
1978年 | 16篇 |
1977年 | 14篇 |
1976年 | 17篇 |
1971年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
41.
Giovanni Melioli Editta Baldini Maria Cristina Mingari Andrea De Maria Mario Roberto Sertoli Fausto Badellino Pier Luigi Percivale Alessandra Catturich Sergio Bertoglio Dario Civalleri Leonardo Santi Lorenzo Moretta 《International journal of cancer. Journal international du cancer》1989,43(2):231-234
In the course of a phase I trial, in which recombinant IL-2 (rIL-2) was infused intraperitoneally (i.p.) in patients with peritoneal carcinomatosis, we evaluated the effect on "tumor-associated lymphocytes" (TAL) isolated from the ascitic fluid. No major changes in the percentages of cells expressing the CD3, CD4, CD8, Leu-7, OKM1 and WT-31 antigens were detected either in TAL or in peripheral blood lymphocytes (PBL) after 7 days of rIL-2 infusion. In contrast the percentages of TAL (but not PBL) expressing surface IL-2 receptor (Tac), or LAK-1 antigen were sharply increased. Analysis of cytolytic functions showed a potentiation of the lytic activity against natural-killer (NK) sensitive K562 target cells and the de novo appearance of lytic activity against fresh melanoma cells. In one patient IFN-gamma was detected in the ascitic fluid following rIL-2 infusion. T-cell clones derived from the patient were analyzed for the IFN-gamma production. While only approximately 40% of PB-derived control clones produced medium to low amounts of IFN-gamma, all of the TAL-derived clones produced medium to high amounts of the lymphokine. 相似文献
42.
Ilaria Marigo Luigi Dolcetti Paolo Serafini Paola Zanovello Vincenzo Bronte 《Immunological reviews》2008,222(1):162-179
Summary: Emerging evidence indicates that the Achilles' heel of cancer immunotherapies is often the complex interplay of tumor-derived factors and deviant host properties, which involve a wide range of immune elements in the lymphoid and myeloid compartments. Regulatory lymphocytes, tumor-conditioned myeloid-derived suppressor cells (MDSCs), tumor-associated macrophages, and dysfunctional and immature dendritic cells take part in a complex immunoregulatory network. Despite the fact that some mechanisms governing tumor-induced immune tolerance and suppression are starting to be better understood and their complexity dissected, little is known about the diachronic picture of immune tolerance. Based on observations of MDSCs, we present a time-structured and topologically consistent idea of tumor-dependent tolerance progression in tumor-bearing hosts. 相似文献
43.
Crosignani Pier Giorgio; De Cecco Luigi; Gastaldi Attilio; Venturini Pier Luigi; Oldani Sabina; Vegetti Walter; Semino Antonio; Commare Paolo La; Vercellini Paolo 《Human reproduction (Oxford, England)》1996,11(12):2732-2735
An open-label randomized pilot study was conducted to evaluatethe efficacy and acceptability of 6 months treatment with leuprolidein a 3-monthly versus a monthly i.m. depot injection for therelief of chronic pelvic pain in women with endometriosis. Atotal of 30 women aged 18-38 years were allocated to the 3-monthlydepot arm (n = 15) or to the monthly depot arm (n = 15) afterlaparoscopic diagnosis of pelvic endometriosis. Mean (SD) deepdys-pareunia scores according to a 03 point verbal ratingscale decreased from 1.8 (0.9) at baseline to 1.3 (0.7) at theend of treatment in the 3-monthly depot group and from 2.1 (1.2)to 13 (0.7) in the monthly depot group. Correspondingvalues in non-menstrual pain scores fell from 2.1 (0.6) to 1.1(03), and from 2.1 (0.8) to 1.2 (0.4) respectively, withoutstatistically significant differences between the groups. Serumluteinizing hormone (LH) and 17-oestradiol concentrations weresignificantly suppressed at 12 and 24 weeks compared with baselinevalues, without differences between the groups. The monthlydepot caused a slightly more marked inhibition of serum folliclestimulating hormone (FSH) levels with respect to the 3-monthlypreparation. Mean (SD) endometriosis scores at baseline andat 6-month follow-up laparoscopy were respectively 32.8 (25.1)and 12.2 (9.3) in the 3-monthly depot group and 29.0 (22.7)and 13.1 (15.3) in the monthly depot group (paired Mest, P 0.05). Mean percentage decrease in lumbar spine bone mineraldensity was 5.2% in the former and 4.9% in the latter subjects.In the 3-monthly depot group, 13 women graded the tolerabilityof their treatment schedule as good compared withseven in the monthly depot group (2 = 5.40, P = 0.02). 相似文献
44.
Luigi Marazzini Andrea Pelucchi Marina Bozzoni Berardino Mastropasqua Erminio Longhini 《Journal of clinical immunology》1985,5(3):195-203
The role of adrenergic mechanism in the pathogenesis of allergic disease is controversial. Recent experimental and clinical reports have suggested that -adrenergic blockade impairs and stimulation enhances extrarenal potassium uptake in humans. This led us to study the effect of the intravenous administration of salbutamol, a specific -2-adrenergic agonist, on serum potassium in 9 healthy subjects and in 23 patients with allergic asthma and/or rhinitis. Serum potassium fell significantly and reached a peak decline at the end of venous infusion in all the normal subjects. Seventeen atopic subjects showed a lower or absent serum K+ decrement: there was no difference between asthmatic and rhinitic patients. There was no relation among the salbutamol-induced serum potassium decrement, serum glucose increment, blood pressure and heart-rate changes, and nonspecific bronchial reactivity. These findings suggest that -2-adrenergic hyporesponsiveness is present only in some allergic patients. 相似文献
45.
Peripheral blood dendritic cells increase in kidney-transplant patients without rejection 总被引:2,自引:0,他引:2
Saresella M Marini M Guerini F Ferrante P Della Bella S Chiaramonte S Riva A 《Clinical immunology (Orlando, Fla.)》2004,110(2):191-3; author reply 194
46.
Maria Rosaria Raspollini Gianni Amunni Alessandro Villanucci Francesca Castiglione Duccio Rossi Degl'Innocenti Gianna Baroni Milena Paglierani Gian Luigi Taddei 《Applied immunohistochemistry & molecular morphology》2006,14(2):181-186
The bcl-2 protein is a membrane protein involved in prolonging cell survival by inhibiting apoptosis. The HER-2 oncogene, which is located on chromosome 17 and encodes for a tyrosine-kinase growth factor receptor, is amplified and HER-2/neu is overexpressed in 25% to 30% of breast carcinomas. The authors analyzed the bcl-2 expression and the bcl-2 gene and HER-2/neu overexpression and amplification in FIGO stage IIIC, serous, G3, ovarian carcinomas obtained from living patients who had no evident disease 5 years after primary treatment compared with ovarian carcinomas obtained from patients, matched for stage, grade of differentiation, and treatment, who had died of progression of disease no later than 2 years after primary treatment. bcl-2 overexpression was statistically correlated with progression of disease during first-line chemotherapy (P=0.021). The HER-2/neu status was found not to correlate with progression of disease during first-line chemotherapy. Both bcl-2 and HER-2/neu expression were not statistically associated with the clinical outcome of ovarian cancer patients. Gene amplification of the HER-2/neu chromosome 17 was found in all the HER-2/neu, 3+ score, positive-staining ovarian carcinomas. None of the analyzed samples revealed a translocation t(14;18)(q32;q21) in the bcl-2 gene. The knowledge of additional prognostic or even predictive factors, such as bcl-2 expression, in patients with advanced ovarian carcinoma before the primary chemotherapeutic treatment may help in the management of patients who require a more tailored treatment. In addition, the gene amplification of the HER-2/neu suggests that HER-2 is a potential target for treatment in ovarian cancer. 相似文献
47.
Raspollini MR Castiglione F Garbini F Villanucci A Amunni G Baroni G Boddi V Taddei GL 《International journal of surgical pathology》2005,13(2):135-142
We analyzed in advanced ovarian serous G3 carcinoma the correlation between epidermal growth factor receptor (EGFR) overexpression and tumor angiogenesis and their relation with clinical outcome. Microvessel density (MVD) and vascular endothelial growth factor (VEGF) were statistically correlated with disease-free interval and death from disease both in univariate and multivariate analyses while EGFR expression was not correlated with clinical outcome. MVD was significantly associated with progression of disease during chemotherapy while VEGF and EGFR expression were not correlated with responsiveness to chemotherapy (Fisher's exact test). VEGF expression was correlated with MVD (Fisher's exact test). EGFR showed a trend to correlation with MVD. Further studies focusing on the use of angiogenesis inhibitors in addition to EGFR inhibitors on ovarian carcinoma cells may produce therapeutic strategies in the selection of tailored therapies in ovarian cancer patients. 相似文献
48.
Arbustini E Grasso M Ansaldi S Malattia C Pilotto A Porcu E Disabella E Marziliano N Pisani A Lanzarini L Mannarino S Larizza D Mosconi M Antoniazzi E Zoia MC Meloni G Magrassi L Brega A Bedeschi MF Torrente I Mari F Tavazzi L 《Human mutation》2005,26(5):494
Marfan Syndrome (MFS) is an autosomal dominant disorder of the connective tissue due to mutations of Fibrillin-1 gene (FBN1) in more than 90% of cases and Transforming Growth Factor-Beta-Receptor2 gene (TGFB2R) in a minority of cases. Genotyping is relevant for diagnosis and genotype-phenotype correlations. We describe the FBN1 genotypes and related phenotypes of 81 patients who were referred to our attention for MFS or Marfan-like phenotypes. Patients underwent multidisciplinary pertinent evaluation in the adult or paediatric setting, according to their age. The diagnosis relied on Ghent criteria. To optimise DHPLC analysis of the FBN1 gene, all coding regions of the gene were directly sequenced in 19 cases and 10 controls: heterozygous amplicons were used as true positives. DHPLC sensitivity was 100%. Then, DHPLC was used to screen 62 other cases. We identified 74 FBN1 mutations in 81 patients: 64 were novel and 17 known. Of the 81 mutations, 41 were missense (50.6%), 27, either nonsense or frameshift mutations and predicted a premature termination codon (PTC) (33%), 11 affected splice sites (13.6%), and two predicted in-frame deletions (2.5%). Most mutations (67.9%) occurred in cbEGF-like modules. Genotype was clinically relevant for early diagnosis and conclusion of the diagnostic work-up in patients with incomplete or atypical phenotypes. 相似文献
49.
A case of somatostatin-producing pancreatic tumor associated with severe insulindependent diabetes mellitus and ketoacidotic
coma is reported. The tumor, a 10-cm expansile mass arising from the pancreatic tail of a 70-yr-old woman, was first detected
by ultrasonography, performed because of abdominal pain, and subsequently confirmed by computed tomography and fine-needle
tumor aspiration. Pathologic investigation showed a predominatly solid-trabecular structure with scattered microacini and
psammomatous bodies. A large proportion of tumor cells expressed somatostatin and/or calcitonin. Following resection of the
primary tumor and three peripancreatic lymph nodes with metastases, the patient recovered rapidly from her diabetic syndrome
and remained in substantially good health during a subsequent 8-yr follow-up period, without evidence of tumor recurrence. 相似文献
50.