晶状体蛋白αB对卵巢癌细胞恶性行为的影响

目的 研究晶状体蛋白αB(αB-crystallin)在卵巢癌组织中的表达及其对卵巢癌细胞SKOV3增殖及迁移侵袭能力的影响.方法 免疫组织化学法检测人正常卵巢组织和上皮性卵巢癌组织中αB-crystallin表达;针对蛋白αB-crystallin的基因(CRYAB基因)设计合成靶向小干扰RNA(smallinterfering RNA,siRNA)重组慢病毒,并感染人卵巢浆液性乳头状囊腺癌细胞株SKOV3;流式细胞仪、平板克隆、MTT检测细胞凋亡和增殖;Transwell检测细胞迁移和侵袭能力;Western blot检测基质金属蛋白酶-2 (MMP-2)和基质金属蛋白酶-9(MMP-9)的表达.结果 αB-crystallin在卵巢癌组织中表达高于正常卵巢组织,在Ⅱ型卵巢癌组织中呈明显高表达(P＜0.01),稳转CRYAB siRNA慢病毒后SKOV3细胞内αB-crystallin蛋白表达降低64％,其凋亡和增殖无明显改变,但SKOV3细胞迁移和侵袭能力明显受到抑制(P＜0.01),同时蛋白MMP-2和MMP-9表达下调(P＜0.01).结论 αB-crystallin能够影响卵巢癌SKOV3细胞浸润转移,其在Ⅱ型卵巢癌组织中的高表达可能与其高侵袭潜能相关.     

中国药品不良反应热点主题可视化研究

目的 利用文献计量分析方法探究国内核心期刊药品不良反应（adverse drug reaction，ADR）文献的研究热点、关键点、薄弱点、特殊点以及整体结构等，以期为ADR监测者和研究者把握本领域的研究热点与发展态势提供参考和依据。方法 收集国内核心期刊ADR文献，对关键词进行抽取和统计，利用聚类分析、社会网络分析等方法对高频关键词进行可视化表达。结果 经检索和筛选确定12 527篇文献作为样本，包括关键词50 576个。45个高频关键词被划分为7类，关键词网络密度为0.59。结论 中国核心期刊ADR文献形成了以“合理用药”“不良反应监测”等为核心的研究热点，“儿童”成为新兴的研究热点。“合理用药”是本领域的关键点，其在网络沟通中的“桥梁作用”最为明显。“过敏性休克”“儿童”“肝损害”等是本领域的特殊点。从整体来看，中国核心期刊ADR文献研究已基本成型，但核心主题内容过多且繁杂，尚未形成有一定深度且界限清晰的核心主题。     

磁共振检查辅助隐匿性宫颈癌的诊断及处理6例临床病例报道

目的：探讨磁共振检查（magnetic resonance imaging,MRI）对隐匿性宫颈癌的诊断价值。方法：收集6例绝经晚期宫颈癌患者、有性交出血或阴道流血病史、临床高度怀疑为宫颈浸润癌,但常规宫颈癌三阶梯检查资料（细胞学检查、阴道镜检查及宫颈组织活检）多次未找到病理学“浸润癌”的证据,盆腹腔MRI明确显示宫颈浸润癌,经手术后病理证实。结果：6例患者MRI图像上均找到明确宫颈肿瘤,肿瘤与宫颈基质、子宫内膜及宫旁脂肪有良好的对比,MRI测量肿瘤大小与手术后测量结果符合率为88.5%[（2.370±1.109） cm vs. （2.250±1.084） cm,P ＞0.05]。结论：因价格因素,MRI尚未纳入宫颈癌诊断的常规检查,但对于绝经后阴道流血等有明确症状、常规三阶梯检查未找到病理依据,而临床高度怀疑宫颈浸润癌患者,辅助MRI能直观、准确显示宫颈癌病灶大小及侵犯范围,有利于及时明确诊断,制定适宜的治疗方案。     

顺铂联合紫杉醇分次给药抑制卵巢癌细胞SKOV3的种群恢复

目的：比较顺铂（cisplatin,CDDP）与紫杉醇（paclitaxel,PTX）不同的给药方案对卵巢癌细胞种群恢复的影响。方法：（1）CDDP、PTX或CDDP联合PTX分别于第1天（D1）或D1及D8处理卵巢癌SKOV3细胞;（2）倒置显微镜观察不同处理组中,SKOV3细胞凋亡与种群恢复的情况;（3）CCK-8法分别测定不同处理组在D7和D21时SKOV3细胞的吸光度（absorbance,A）值。结果：（1）单用CDDP组中,单次给药组与分次给药组相比较,二者对SKOV3细胞的凋亡与种群恢复的影响无明显差异（P=0.970）;（2）单用PTX组中,与单次给药组相比,分次给药组明显抑制了SKOV3细胞的种群恢复（P=0.000）;（3）CDDP联合PTX组中,分次给药组较单次给药组对SKOV3细胞的种群恢复明显抑制（P=0.000）,并且在分次给药组中,CDDP联合PTX组较单用PTX组明显抑制SKOV3细胞的种群恢复（P=0.000）。结论：CDDP联合PTX分次给药较单次给药可能通过抑制卵巢癌细胞的种群恢复从而抑制卵巢癌的耐药或复发。     

18F-FDG PET/CT在结直肠腺癌及癌前病变中的诊断价值

背景 结直肠癌发病率、死亡率高,早诊早治十分重要.但常规检查难以鉴别诊断直径≥20 mm的进展期腺瘤,造成了患者经济和心理的双重负担.目的 探讨18F-FDG PET/CT鉴别直径≥20 mm结直肠癌癌前病变与结直肠腺癌的诊断效能及相关影响因素.方法 回顾性分析2010年1月-2020年1月于解放军总医院第一医学中心明...     

Clinical experience of using a novel self‐help inflatable balloon to prevent esophageal stricture after circumferential endoscopic submucosal dissection

Although endoscopic submucosal dissection (ESD) is gradually becoming a first‐line treatment for superficial esophageal neoplasms (SEN), strictures occur in almost 100% of cases after circumferential ESD. A standard method to prevent stricture has not been established. Thus, we propose a novel self‐help inflatable balloon to prevent stricture. The new balloon was used by the patients themselves at home (4–5 times a day, duration of each procedure was approximately 15–20 min), and was removed when the defects were almost healed. From January 2018 to September 2018, eight patients who received circumferential ESD for SEN and underwent a novel self‐help inflatable balloon to prevent stricture were enrolled. Median size of the mucosal defects was 76.3 mm (range: 50–90 mm). Median time for removing the self‐help inflatable balloon was 94.6 days (range, 71–119 days). Only one (12.5%) patient experienced stricture, and three endoscopic balloon dilation sessions were carried out for this patient. All patients tolerated the balloon well, and none experienced perforation or delayed bleeding. The self‐help inflatable balloon seems to show a high preventive effect against stricture in patients whose mucosal defect was no longer than 100 mm in length after esophageal circumferential ESD. This method is economic, feasible, and safe.     

A Mixed Wavelet-Learning Method of Predicting Macroscopic Effective Heat Transfer Conductivities of Braided Composite Materials

In this paper, a novel mixed wavelet-learning method is developed for predicting macroscopic effective heat transfer conductivities of braided composite materials with heterogeneous thermal conductivity. This innovative methodology integrates respective superiorities of multi-scale modeling, wavelet transform and neural networks together. By the aid of asymptotic homogenization method (AHM), off-line multi-scale modeling is accomplished for establishing the material database with high-dimensional and highly-complex mappings. The multi-scale material database and the wavelet-learning strategy ease the on-line training of neural networks, and enable us to efficiently build relatively simple networks that have an essentially increasing capacity and resisting noise for approximating the high-complexity mappings. Moreover, it should be emphasized that the wavelet-learning strategy can not only extract essential data characteristics from the material database, but also achieve a tremendous reduction in input data of neural networks. The numerical experiments performed using multiple 3D braided composite models verify the excellent performance of the presented mixed approach. The numerical results demonstrate that the mixed wavelet-learning methodology is a robust method for computing the macroscopic effective heat transfer conductivities with distinct heterogeneity patterns. The presented method can enormously decrease the computational time, and can be further expanded into estimating macroscopic effective mechanical properties of braided composites.     

生产性噪声对人体健康的影响

用统一印制的接触噪声作业体检册对暴露在８０～１０１ｄＢ（Ａ）生产环境中的７１名作业者进行调查，听力检查在本底噪声值≤３０ｄＢ（Ａ）的静室内进行，传导性耳聋加做诱发电位。发现接噪者自觉症状以头昏为最多，头痛、耳鸣次之，其它如胃痛、食欲不振、胸闷等症状各占一定比例；神经衰弱是接噪者明显的主观症状，阳性者３９人（５４．９３％），女（占６７．６５％）明显多于男（４３．２４％），稳态组与脉冲组相比差异有显著性；接噪早期有低血压倾向，心电图发现异常较多，但以窦性心律不齐（７．０４％）和窦性心动过缓（５．６３％）为主；查出噪声性耳聋６人（８．４５％），高频听损３４人（４３．６６％），听力图发现脉冲组听力Ｖ型下降较稳态组明显，脉冲组平均听损较稳态组大近４ｄＢ（Ａ），表明脉冲噪声对人体的听觉系统损害较稳态噪声严重