Assessment of aortoiliac obstructive disease by Doppler spectrum analysis of blood flow velocities in the common femoral artery at rest and during reactive hyperemia

Aortoiliac obstructive disease was assessed by Doppler spectrum analysis of blood flow velocities in the common femoral artery measured both at rest and during reactive hyperemia. The intraarterial femoral artery pressure measured at rest and during reactive hyperemia served as the "gold standard" for the definition of a hemodynamically significant aortoiliac stenosis. Our results, obtained from 93 patients (136 limbs), showed that differences between Doppler spectra obtained from limbs with a hemodynamically significant aortoiliac stenosis and those from limbs without were more pronounced during reactive hyperemia than at rest. The best assessment of aortoiliac obstructive disease could be obtained with a combination of parameters derived from Doppler spectra measured at rest and during reactive hyperemia. With these parameters, obtained by multivariate analysis, 85% of the limbs were diagnosed correctly compared with the gold standard. If angiographic data were added to the gold standard, even 91% of the limbs were diagnosed correctly. We conclude that analysis of Doppler spectra-obtained noninvasively from the common femoral artery at rest and during reactive hyperemia provides an accurate hemodynamic assessment of the aortoiliac segment.     

Tear of the distal biceps branchii tendon: A new method of ultrasound evaluation

Tear of the distal biceps brachii tendon is an uncommon injury. Ultrasound evaluation of the distal tendon using an anterior approach is often difficult because of technical factors. We describe a new method of ultrasound evaluation of the distal biceps tendon insertion. This involves a posterior approach with the forearm pronated. With pronation of the forearm, the radial tuberosity faces posteriorly, bringing the distal biceps tendon insertion into view. A surgically proven case of distal biceps tendon tear is presented to illustrate our technique.     

培养创新型临床医学人才的思考

文章从创新及创新型人才的概念入手，通过调研指出，目前临床医学人才培养存在的主要问题，提出在新时期要转变教育教学观念、强化创新意识教育、增强创新思维训练、着力创新人格塑造，以培养创新型临床医学人才。     

YD383、YD439对JAK-STAT6信号传导通路作用的特异性研究

目的:探讨化合物YD383和YD439对STAT6信号传导通路的作用是否具有特异性.方法:通过瞬时转染的方法将分别依赖于STAT1和STAT6活性的带有荧光素酶报告基因的质粒转染至HeLa细胞株,两种细胞株在各自相应的细胞因子(IFN-γ或IL-4)刺激下激活JAK-STAT1或JAK-STAT6信号传导通路.通过测定荧光素酶的活性来评价细胞株在化合物存在的情况下报告基因的表达.结果:YD383和YD439均能降低相应细胞因子刺激引起的报告基因的表达,并且具有剂量依赖性.随着化合物剂量的增加,报告基因的表达降低(P＜0.05).但化合物引起的两种转染细胞报告基因表达降低的差异比较无统计学意义(P＞0.05).结论:两种化合物对JAK-STAT1和JAK-STAT6信号传导通路均有抑制作用,对JAK-STAT6通路的抑制不具有特异性.     

microRNA调控NK细胞KIR3DL1表达初探

目的初步探寻人外周血自然杀伤细胞（Nature Killer,NK）杀伤细胞免疫球蛋白样受体（Killer Cell Immnoglobulin-Like Receptor,KIR3DL1）表达可能存在的microRNA（miR）调控机制。方法利用生物信息学方法,从miR信息库中筛选出可能与KIR3DL1相关的miRs,构建含KIR3DL1 3’非翻译区（UTR）的PGL3质粒,分别将含相应miR的PcDNA3.0质粒与前者共转染293T细胞,通过荧光素酶报告实验及之后的突变实验筛选出可能调控KIR3DL1的miR。结果通过TARGET SCAN信息库筛选了miR-146b等10个miR;转染miR-146b后,荧光素酶活性下降最多（61.3%）,突变其KIR3DL1 3’UTR靶位点后荧光素酶活性恢复（91.4%）。结论 miR-146b可与KIR3DL1’UTR在靶位点特异结合,很可能参与KIR3DL1表达的调控。     

氯化镧对内毒素诱导巨噬细胞肿瘤坏死因子表达的影响

目的　探讨稀土化合物氯化镧对内毒素效应的影响。　方法　分离培养小鼠腹腔巨噬细胞 (M) ,用内毒素 (lipopolysaccharide ,LPS)对其进行诱导实验。采用ELISA法及SYBRGreen荧光定量RT PCR方法 ,观察氯化镧对LPS诱导的小鼠M肿瘤坏死因子α(TNFα)分泌及TNFαmRNA表达的影响。BALB/C小鼠随机分为 2组 ,分别给予致死量LPS及经氯化镧处理的LPS ,观察 7d内小鼠死亡率。　结果　经氯化镧处理的LPS诱导的小鼠 ,MTNFα分泌量及TNFαmRNA表达水平均明显低于LPS组 (P <0 .0 1)。经氯化镧处理的致死量LPS攻击小鼠 ,其死亡率明显低于LPS对照组。　结论　氯化镧具有降低内毒素毒性、抑制LPS诱导小鼠MTNFα分泌及TNFαmRNA表达的作用     

妊娠与未妊娠患者超排卵周期第10天子宫内膜基因表达差异分析

目的对内膜修整后所取得的内膜组织按妊娠结果的不同分组进行分子水平的分析和检测,寻找合适的内膜发育分子标志物,早期预测辅助生殖技术(ART)患者的妊娠结果。方法随机选择10例接受增殖期子宫内膜修整术患者,其B超及组织学检查结果表明内膜处于相同发育阶段,按妊娠结果分为妊娠组与未妊娠组,抽提每例的子宫内膜组织总RNA,4例妊娠患者和3例未妊娠患者的内膜组织RNA符合芯片检测要求,使用Affymetrix U133 plus 2.0基因芯片进行表达谱分析。结果妊娠组与未妊娠组子宫内膜组织具有218个差异表达基因(≥2倍),其中177个基因在未妊娠组高表达,41个基因在妊娠组高表达。差异基因主要涉及代谢、细胞通讯和胞内过程调控等生物学过程,参与离子结合、信号传导和物质转运等重要功能。结论妊娠组与未妊娠组的增殖期子宫内膜细胞表达在转录组水平上差异明显,某些重要差异表达基因(LAMA4i、ntegrinα6和MMP1)有可能成为便利的早期预测ART患者妊娠结果的分子标志物。