Association between serum cystatin C and early impairment of cardiac function and structure in type 2 diabetes patients with normal renal function

BackgroundType 2 diabetes mellitus (T2DM) patients may have cardiac remodeling and dysfunction from the early stage of disease. This study aimed to determine the association between cystatin C (CysC) and early cardiac functional or structural impairment in T2DM patients without renal dysfunction.MethodsA total of 1135 T2DM patients without renal dysfunction and known heart diseases were included in our study. Cardiac function and structure were evaluated by echocardiography. Patients were diagnosed as left ventricular hypertrophy (LVH), impaired left ventricular (LV) diastolic function, and categorized into four different LV geometry patterns including normal, concentric remodeling, concentric hypertrophy, and eccentric hypertrophy.ResultsIn multivariate linear regression analyses, CysC was positively associated with interventricular septum, LV mass index, left atrial volume index, E/e'' ratio, and negatively associated with Tissue Doppler e'', E/A ratio (p < .05). As a continuous variable, increasing CysC levels were associated with prevalence of LVH (OR: 1.47, 95% confidence interval [CI]: 1.22–1.77), impaired LV diastolic function (OR: 1.58, 95% CI: 1.33–1.87), concentric hypertrophy (OR: 1.54, 95% CI: 1.23–1.93) and eccentric hypertrophy (OR: 1.34, 95% CI: 1.00–1.80) according to multivariate logistic regression analyses. While as a categorical variable, the highest CysC quartile (CysC > 1.04 mg/L) was associated with LVH (OR: 2.95, 95% CI: 1.74–5.00), impaired LV diastolic function (OR: 4.09, 95% CI: 2.54–6.60), and concentric hypertrophy (OR: 3.26, 95% CI: 2.05–5.18).ConclusionsCysC was significantly associated with early LV remodeling and cardiac functional impairment in T2DM patients with normal renal function. It could be a reliable and convenient biomarker detecting early impairment of cardiac function and structure in T2DM patients.     

酶处理急性分离技术用于研究果蝇成虫中枢神经细胞钾电流

目的 建立急性分离果蝇成虫中枢神经细胞技术,并记录分析其钾电流。方法 取S、R或H品系果蝇成虫3—5只,分离出果蝇脑组织。胶原酶消化处理后,加入10％的胎牛血清果蝇培养基终止酶反应。巴氏吸管吹打,细胞悬液静置1h后用于电生理实验。全细胞电压钳记录并分析其外向钾电流。结果 酶消化法成功急性分离出果蝇成虫中枢神经细胞。85％以上为Ⅱ型神经细胞,少部分为Ⅰ型和Ⅲ型神经细胞。记录到快失活与持续复合型、慢失活型、快失活和持续型四种全细胞钾电流。结论 本技术可成功急性分离三品系果蝇成虫中枢神经细胞,并记录到四种钾电流。     

残疾人士的健康、心理和生活状况调查分析

目的　了解残疾人现时的健康、心理和生活状况,为制订全面社区康复计划提供科学依据。方法　进行随机抽样的入户访谈式调查。结果　肢残者所占的比例最大,其次是听力语言残疾,视残居第三位;以疾病、先天遗传和意外伤害为三大致残原因,而车祸是意外伤害的第一致残原因;不同年龄阶段各类残疾的构成存在显著性差异;不同文化程度的残疾人心理状态评价存在显著性差异;大多数残疾人对福利政策感到不满意或不太满意。结论　普遍开展社区康复服务,增加残疾人的就业机会,完善我国的残疾人福利政策等。     

深圳市南山区8～24月龄儿童基础免疫延迟接种影响因素的调查分析

采用按容量比例概率抽样法（PPS）随机抽取深圳市南山区30个居委会的210名8～24mon龄儿童,以被抽中儿童及其父母作为调查对象,对他们进行问卷调查。调查内容包括：儿童、父母一般情况,父母有关计划免疫知识和健康信念,计划免疫服务评价,儿童计划免疫情况等。对其中的184份有效问卷进行了分析,结果184名儿童“四苗”及时接税率为45．1％,以及时接种者为对照组,延迟接种者为观察组,进行Logistic回归分析。单因素分析结果显示：儿童出生在外地、是暂住户口、在外地居住3个月以上者、亲友中无从事医疗工作者是儿童“四苗”接种延迟的危险因素（P＜0.05）,父母亲文化程度越低,儿童出现“四苗”接种延迟的可能性越高,父母对有关的知识、信念得分愈高儿童发生“四苗”接种延迟的危险性也就愈小（P＜0．05）;其余因素对“四苗”接种延迟无显著影响。进一步的多因素Logstic回归分析,儿童出生地、亲戚朋友有无从事医疗工作、户口类型、信念得分四因素依次进入多元logisitic回归方程。     

19号染色体长臂部分重复致癫痫伴发育落后1例遗传及表型分析

目的:探讨1例癫痫伴运动发育落后的患儿的遗传学病因,为临床诊断和遗传咨询提供依据。方法:收集2020年1月因癫痫伴运动发育落后到河南省人民医院就诊的1例患儿及其父母外周血各2 mL,采用常规G显带分析患儿及其父母的外周血染色体核型,采用微阵列比较基因组杂交(aCGH)技术对患儿及其父母进行染色体片段重复/缺失进行分析。...     

Effect of acupuncture on adrenocortical hormone production: I. Variation in the ability for adrenocortical hormone production in relation to the duration of acupuncture stimulation.

The effect of acupuncture on adrenocortical hormone production in adult female albino rabbits of New Zealand strain was investigated. There was significant increase compared to the control cortisol and corticosterone following application of acupuncture at the locus Tsu-San-Li. In the non-locus group, such treatment resulted in only a transient raise followed by a significant fall in cortisol and corticosterone. Acupuncture treatment with electric current for 1 hour proved to be unique in that it gave rise to no biphasic change in the 14C uptake of the steroids. These results indicate that adrenal production of corticosterone and cortisol was enhanced by acupuncture and electroacupuncture at the Tsu-San-Li locus.