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Al-Nakkash L Clarke LL Rottinghaus GE Chen YJ Cooper K Rubin LJ 《The Journal of nutrition》2006,136(11):2785-2790
Genistein, a naturally occurring isoflavone, augments in vitro epithelial anion transport via activation of the cystic fibrosis transmembrane conductance regulator chloride channel. In this study, we examined whether chronic dietary exposure to 600 mg/kg genistein (600 G) for 1 mo would stimulate anion secretion across wild-type (Wt, normal) murine intestine. Anion secretion was assessed in freshly excised segments of murine jejuna by measuring short circuit current (I(sc)) and comparing with jejunal segments from mice fed 0 mg/kg genistein (0 G). Basal and forskolin-stimulated anion secretions were augmented (P < 0.05) in female but not in male mice fed 600 G, compared with their counterparts fed 0 G. Serum genistein concentrations were greater in both female and male mice fed 600 G (approximately 3.5-6.9 micromol/L) than those fed 0 G (approximately 100 nmol/L). Anion substitution experiments and bumetanide-sensitivity demonstrated that chloride was the major anion mediating the increased secretion. A smaller bicarbonate component was not augmented by consumption of the genistein diet. These data indicate that chronic exposure to dietary genistein stimulates a sex-dependent increase in basal and forskolin-stimulated chloride secretion across murine intestine. 相似文献
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After lactoperoxidase-catalyzed iodination of either human sperm surface or human seminal plasma, a 72–76 kdalton component was resolved on SDS-7.5% polyacrylamide gels. The approximate molecular weight of this component was consistent with that of human lactoferrin, a major sperm-coating antigen. Immunoprecipitation of labeled sperm surface and of labeled seminal plasma with specific anti-human lactoferrin antibody identified lactoferrin as a constituent of this 72–76 kdalton component both on human sperm surface and in human seminal plasma. These data show that components which have been inferred to be shared because of similarities in biochemical characteristics also contain immunologically identical components. 相似文献
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The development and preliminary validation of a brief measure of chronic pain impact for use in the general population 总被引:2,自引:0,他引:2
From a biopsychosocial perspective, assessing chronic pain's psychological impact should involve at minimum the measurement of pain severity, functional interference, and pain-related emotional burden. This article details the development of a brief instrument, the 15-item Profile of Chronic Pain: Screen (PCP:S), designed to address these three key elements in a national (US) sample of over 2400 individuals recruited via random digit dialing. Retest reliability, internal consistency, and preliminary validity were excellent. The scales also demonstrated minimal social desirability response bias. A series of confirmatory factor analyses on several distinct samples revealed a stable, 3-factor solution reflecting pain severity, interference, and emotional burden. Finally, national norms were developed by gender and three age groups. In view of its strong psychometric properties, the PCP:S has the potential to serve as a brief, cost-effective assessment tool for identifying individuals whose chronic pain merits more detailed psychosocial evaluation. 相似文献
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Marcelo E Facciuto Caroline Rochon Mahima Pandey Manuel Rodriguez-Davalos Susana Samaniego David C Wolf Leona Kim-Schluger Grigory Rozenblit Patricia A Sheiner 《HPB : the official journal of the International Hepato Pancreato Biliary Association》2009,11(5):398-404