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131.
S Pierno GM Camerino V Cippone J-F Rolland J-F Desaphy A De Luca A Liantonio G Bianco JD Kunic AL George Jr D Conte Camerino 《British journal of pharmacology》2009,156(8):1206-1215
Background and purpose:
Statins and fibrates can produce mild to life-threatening skeletal muscle damage. Resting chloride channel conductance (gCl), carried by the ClC-1 channel, is reduced in muscles of rats chronically treated with fluvastatin, atorvastatin or fenofibrate, along with increased resting cytosolic calcium in statin-treated rats. A high gCl, controlled by the Ca2+-dependent protein kinase C (PKC), maintains sarcolemma electrical stability and its reduction alters muscle function. Here, we investigated how statins and fenofibrate impaired gCl.Experimental approach:
In rats treated with fluvastatin, atorvastatin or fenofibrate, we examined the involvement of PKC in gCl reduction by the two intracellular microelectrodes technique and ClC-1 mRNA level by quantitative real time-polymerase chain reaction. Direct drug effects were tested by patch clamp analysis on human ClC-1 channels expressed in human embryonic kidney (HEK) 293 cells.Key results:
Chelerythrine, a PKC inhibitor, applied in vitro on muscle dissected from atorvastatin-treated rats fully restored gCl, suggesting the involvement of this enzyme in statin action. Chelerythrine partially restored gCl in muscles from fluvastatin-treated rats but not in those from fenofibrate-treated rats, implying additional mechanisms for gCl impairment. Accordingly, a decrease of ClC-1 channel mRNA was found in both fluvastatin-and fenofibrate-treated rat muscles. Fenofibric acid, the in vivo metabolite of fenofibrate, but not fluvastatin, rapidly reduced chloride currents in HEK 293 cells.Conclusions and implications:
Our data suggest multiple mechanisms underlie the effect of statins and fenofibrate on ClC-1 channel conductance. While statins promote Ca2+-mediated PKC activation, fenofibrate directly inhibits ClC-1 channels and both fluvastatin and fenofibrate impair expression of mRNA for ClC-1. 相似文献132.
133.
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135.
Dr. Rade B. Vukmir MD JD FCCP FACEP 《European journal of trauma and emergency surgery》2008,34(3):261-266
Abstract
Background: This work attempted to define the care and course of those most severely affected patients in the setting of blunt chest trauma,
who had hypotension refractory to routine fluid resuscitation.
Methods: Twenty-three critically ill blunt trauma ICU patients were resuscitated and enrolled with ongoing hypotension required placement
of a pulmonary artery catheter. The REF?Explorer (Baxter, Edwards, Anaheim, CA) catheter was placed in the right heart measuring pressure, volume and oxygen utilization
information, as well as recording Injury Severity Score, EKG, CXR, CPK/MB and echocardiography over the initial 72-h time
period.
Results: There were an approximately 2,300 Level I trauma patients admitted annually over a 4-year period with an overall mortality
rate of 4.3% (100) patients with 3.4% (79) patients “ruling in” with elevated cardiac enzymes, associated with an increased
mortality rate of 6.7% (p < 0.05). The 23 patients were male (17, 74%), mean age 41.2 years, with no past medical history
(19, 83%), in a motor vehicle accident (21, 91%), with pulmonary injury (9, 39%), undergoing celiotomy in (10, 44%). They
presented with moderate to severe trauma acuity defined as mean GCS of 8.6, TS of 11.3, and ISS of 34 with an increased mean
hospital stay of 15 days versus 6 days in the ICU; and a 26 days versus 10 days overall stay for those with myocardial contusion
(p < 0.05). Analysis of diagnostic variables found an abnormal EKG in (21, 91%), CXR in (20, 87%) and echocardiogram in (8,
37%). The total CPK was found to be elevated, mean 2,219 (204–8,278 U/l), while the MB fraction was normal 2.3 ± 1.3%. Invasive
cardiac monitoring found an increase in CO of 1.6 l/min from 5.9 to 7.8 l/min during the first 24 h of recovery. Survival
was worsened with increased ISS (29 vs. 43) p < 0.02, but improved with longer ICU (17 vs. 8) p < 0.03 and hospital (39 vs.
7) p < 0.05 stay in days. The analysis of commonly used diagnostic modalities – EKG, CXR, ECHO, or CO, did not correlate with
survival, but the total CPK was increased in survivors (2,715 vs. 1,432 U/l) p < 0.009.
Conclusion: There is worsened morbidity with a 2-fold increase in ICU LOS and hospital stay, and a 1.5-fold increase in mortality in the
severe myocardial contusion group. The diagnostic dilemma posed by lack of definitive testing continues unresolved after analysis
of routinemodalities – EKG, CXR, ECHO, CPK or CO – failing to yield a “best test”. 相似文献
136.
137.
Shikha Rao MBBS FAAD David Goldberg MD JD FAAD 《Journal of Cosmetic Dermatology》2023,22(10):2765-2768
Background
Neck skin is thinner and has a more delicate dermal layer than facial skin. The studied product was specifically formulated for the neck combining a hydrating delivery system with a trifunctional corrective technology composed of 0.2% pure retinol, 2.5% tripeptide concentrate, and 5.0% glaucine complex to help improvement in signs of aging.Objectives
To evaluate cosmetic and histologic changes 3 months after treatment using immunostains for Type I collagen, Type III collagen, and glycosaminoglycan (GAGS). In addition, overall clinical improvement in photoaged skin was measured by both Griffith's photonumeric photoaging scale, photographic improvement, and questionnaires.Methods
This study was an open-label, blinded clinical trial evaluating a combined retinol, tripeptide, and glaucine containing cream in the treatment of photo-aged skin. The study enrolled a total of 20 healthy male or female subjects, who applied the product for 3 months to their face and neck.Results
Clinical as well histologic changes were consistent with improvement in all 20 subjects.Conclusion
Use of a combined retinol, tripeptide, and glaucine containing cream led to both clinical and histologic improvement of phototoaging. 相似文献138.
139.
David J. Goldberg MD JD Anneke Andriessen PhD Ashish C. Bhatia MD FAAD Michael H. Gold MD FAAD Amy B. Lewis MD Margarita S. Lolis MD FAAD Jason H. Miller MD FAAD Adriana Ros MD FAAD 《Journal of Cosmetic Dermatology》2023,22(11):3026-3032
Introduction
Acne is the most common reason for dermatology consultation in adolescents and young adults. Consultation is often delayed despite unsuccessful self-treatment. Postponing effective treatment places acne sufferers at higher risk for permanent acne scars and post-inflammatory pigment changes.Aim
This review discusses clinical challenges with present therapeutic options for acne treatment and the role of a 1726 nm laser for acne.Methods
Current acne treatment guidelines were reviewed. A literature review was conducted for trials of light-based acne therapy. The selectivity of previous light-based therapies was reviewed.Results
Available acne therapy is effective, but treatment-related side effects are common. Acne treatment guidelines do not include recommendations for light-based treatments. Different types of light-based treatments have been tried but until now no wavelength specifically targeted sebaceous glands.Conclusion
The 1726 nm laser is safe and effective for treating mild to severe acne in all Fitzpatrick skin types. Acne resolution is apparent within the first month and improves for up to 2 years beyond treatment. 相似文献140.
Michael Gold MD FAAD Yoon-Soo Cindy Bae MD FAAD David J. Goldberg MD JD FAAD Sumayah Jamal MD PhD Amy B. Lewis MD Shari Marchbein MD FAAD Adriana Ros DO Uma Santhanam PhD Lisa DiNatale Jaime Emmetsberger PhD 《Journal of Cosmetic Dermatology》2023,22(12):3313-3319