全文获取类型
收费全文 | 455篇 |
免费 | 18篇 |
国内免费 | 22篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 36篇 |
妇产科学 | 3篇 |
基础医学 | 17篇 |
口腔科学 | 9篇 |
临床医学 | 38篇 |
内科学 | 61篇 |
皮肤病学 | 8篇 |
神经病学 | 6篇 |
特种医学 | 120篇 |
外科学 | 45篇 |
综合类 | 30篇 |
预防医学 | 28篇 |
眼科学 | 27篇 |
药学 | 33篇 |
肿瘤学 | 31篇 |
出版年
2023年 | 2篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 8篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 10篇 |
2014年 | 8篇 |
2013年 | 12篇 |
2012年 | 5篇 |
2011年 | 7篇 |
2010年 | 11篇 |
2009年 | 13篇 |
2008年 | 11篇 |
2007年 | 22篇 |
2006年 | 33篇 |
2005年 | 17篇 |
2004年 | 17篇 |
2003年 | 7篇 |
2002年 | 10篇 |
2001年 | 21篇 |
2000年 | 7篇 |
1999年 | 10篇 |
1998年 | 33篇 |
1997年 | 21篇 |
1996年 | 26篇 |
1995年 | 22篇 |
1994年 | 19篇 |
1993年 | 22篇 |
1992年 | 6篇 |
1991年 | 4篇 |
1990年 | 9篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 4篇 |
1986年 | 11篇 |
1985年 | 8篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 13篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 1篇 |
排序方式: 共有495条查询结果,搜索用时 15 毫秒
51.
目的:间充质干细胞具有强大的增殖能力和多向分化潜能,文章对其主要的来源途径予以综述。资料来源:应用计算机检索Medline1991-01/2006-01期间的相关文章,检索词为“mesenchyma stem cells,origin,research progress”,并限定文章语言种类为English。同时计算机检索中国期刊全文数据库1998-01/2006-10期间的相关文章,检索词为“间充质干细胞,来源,研究进展”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:①间充质干细胞的起源。②间充质干细胞研究进展、干细胞的分离及鉴定。排除标准:重复研究、个案报告或Meta分析类文章。资料提炼:共收集到96篇相关文献,40篇文献符合纳入标准,排除的56篇文献为内容陈旧或重复。符合纳入标准的40篇文献中,分别涉及骨髓、肌肉、脐血、胎盘、外周血、脂肪组织、血管及其他来源的间充质干细胞。资料综合:间充质干细胞是属于中胚层的一类多能干细胞,具有强大的增殖能力和多向分化潜能,动物模型试验和临床应用研究也取得了一定的效果。间充质干细胞来源广泛,易于获得,临床上为神经损伤及其他系统的损伤修复提供了更为广泛的途径。结论:间充质干细胞主要来源于骨髓、肌肉、脐血、外周血、胎盘等组织,具有广阔的应用前景。 相似文献
52.
53.
Preparation of factor IX deficient human plasma by immunoaffinity chromatography using a monoclonal antibody 总被引:1,自引:0,他引:1
Goodall AH; Kemble G; O'Brien DP; Rawlings E; Rotblat F; Russell GC; Janossy G; Tuddenham EG 《Blood》1982,59(3):664-670
A murine hybridoma clone is described that grows continuously in culture and produces a monoclonal antibody we have called Royal Free Monoclonal Antibody to factor IX No. 1 (RFF-IX/1). This has high affinity for a coagulation site on factor IX. RFF-IX/1 immobilised on sepharose can be used to deplete factor IX from normal human plasma. This immunoaffinity depleted plasma is indistinguishable from severe Christmas disease plasma and can be used as the substrate in a one stage coagulation assay for factor IX. The affinity column has high capacity and can be regenerated so that large scale production from normal plasma of factor IX deficient plasma as a diagnostic reagent is now feasible. 相似文献
54.
Alterations in neutrophil subpopulations during human hemodialysis or following injection of C5a des arg into rabbits were studied. Whereas baseline peripheral blood neutrophils contained approximately 80% of cells that formed rosettes with IgG-sensitized erythrocytes, neutrophils harvested at the granulocyte nadir (20 min after initiating hemodialysis or the injection of C5a des arg) were markedly depleted of this population. This was seen in a change in ratio of rosette-forming neutrophils (RFN) to non-rosette-forming neutrophils (non-RFN) from 4:1 at 0 time to 1:2 at 20 min. Since non-RFN are less active in assays of adherence and chemotaxis, these alterations in circulating neutrophil populations were reflected in abnormal functional capacity of neutrophils harvested at 20 min. To study the mechanism of RFN depletion, we investigated the ability of C5a des arg to aggregate various human neutrophil suspensions. Unfractionated neutrophils and RFN demonstrated prompt in vitro aggregation in response to C5a des arg, whereas this activated complement fragment induced little aggregation in a population enriched for non-RFN. These results may explain the alterations in neutrophil adherence, chemotaxis, phagocytosis, and bactericidal activity, which have been reported to accompany clinical disorders characterized by in vivo complement activation (i.e., hemodialysis or gram-negative sepsis). 相似文献
55.
Mark DP Willcox 《Clinical & experimental optometry》2019,102(4):350-363
This article summarises research undertaken since 1993 in the Willcox laboratory at the University of New South Wales, Sydney on the tear film, its interactions with contact lenses, and the use of tears as a source of biomarkers for ocular and non‐ocular diseases. The proteome, lipidome and glycome of tears all contribute to important aspects of the tear film, including its structure, its ability to defend the ocular surface against microbes and to help heal ocular surface injuries. The tear film interacts with contact lenses in vivo and interactions between tears and lenses can affect the biocompatibility of lenses, and may be important in mediating discomfort responses during lens wear. Suggestions are made for follow‐up research. 相似文献
56.
Little DM; Farrell JG; Cunningham PM; Hickey DP 《QJM : monthly journal of the Association of Physicians》1997,90(10):641-642
Systemic donor infection is regarded as being an absolute contraindication
to cadaveric organ donation for transplantation. This is largely due to
fear of transmitting pathogenic organisms to the immunosuppressed
recipient. However, due to the current shortage of organs available for
transplantation, clinicians are faced with the option of using organs from
'non-ideal' donors, such as those patients with documented evidence of
infection. We report the successful outcome of six orthotopic liver
transplants, 11 renal transplants, one combined heart lung transplant and
one simultaneous kidney and pancreas transplant with organs from eight
donors in whom bacterial meningitis (n = 7) and acute bacterial
epiglottitis (n = 1) were the antecedent causes of death.
相似文献
57.
<Emphasis Type="Italic">BRCA1</Emphasis> and <Emphasis Type="Italic">BRCA2</Emphasis> mutations in a population-based study of male breast cancer 下载免费PDF全文
Victoria?M?Basham Julian?M?Lipscombe Joanna?M?Ward Simon?A?Gayther Bruce?AJ?Ponder Douglas?F?Easton Paul?DP?PharoahEmail author 《Breast cancer research : BCR》2001,4(1):R2
Background
The contribution of BRCA1 and BRCA2 to the incidence of male breast cancer (MBC) in the United Kingdom is not known, and the importance of these genes in the increased risk of female breast cancer associated with a family history of breast cancer in a male first-degree relative is unclear.Methods
We have carried out a population-based study of 94 MBC cases collected in the UK. We screened genomic DNA for mutations in BRCA1 and BRCA2 and used family history data from these cases to calculate the risk of breast cancer to female relatives of MBC cases. We also estimated the contribution of BRCA1 and BRCA2 to this risk.Results
Nineteen cases (20%) reported a first-degree relative with breast cancer, of whom seven also had an affected second-degree relative. The breast cancer risk in female first-degree relatives was 2.4 times (95% confidence interval [CI] = 1.4–4.0) the risk in the general population. No BRCA1 mutation carriers were identified and five cases were found to carry a mutation in BRCA2. Allowing for a mutation detection sensitivity frequency of 70%, the carrier frequency for BRCA2 mutations was 8% (95% CI = 3–19). All the mutation carriers had a family history of breast, ovarian, prostate or pancreatic cancer. However, BRCA2 accounted for only 15% of the excess familial risk of breast cancer in female first-degree relatives.Conclusion
These data suggest that other genes that confer an increased risk for both female and male breast cancer have yet to be found.58.
CH Reynolds BBSc DP Crewther PhD SG Crewther PhD SJ West BSc 《Clinical & experimental ophthalmology》1998,26(S1):S117-S119
59.
60.
A PIL for every ill? Patient information leaflets (PILs): a review of past, present and future use 总被引:9,自引:0,他引:9
Kenny T; Wilson RG; Purves IN; Clark J Sr; Newton LD; Newton DP; Moseley DV 《Family practice》1998,15(5):471-479
This article reviews the usefulness and importance of written information,
specifically leaflets, being given to patients. Evidence suggesting how
both patient and doctor may benefit from the giving of written information
is reviewed. Identification of good practice relating to the content and
readability of leaflets is discussed. An argument is put forward that the
giving of written information is an under-utilized resource in contributing
to improving patient outcomes but that this may be changing with the
increasing use of patient leaflet databases. The advantages and
disadvantages of computer- generated patient leaflets are discussed and
desirable further areas of research on computer-generated leaflets are
proposed.
相似文献