全文获取类型
收费全文 | 1670篇 |
免费 | 97篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 12篇 |
儿科学 | 79篇 |
妇产科学 | 58篇 |
基础医学 | 236篇 |
口腔科学 | 46篇 |
临床医学 | 166篇 |
内科学 | 323篇 |
皮肤病学 | 80篇 |
神经病学 | 120篇 |
特种医学 | 34篇 |
外国民族医学 | 1篇 |
外科学 | 176篇 |
综合类 | 35篇 |
一般理论 | 3篇 |
预防医学 | 113篇 |
眼科学 | 23篇 |
药学 | 145篇 |
中国医学 | 14篇 |
肿瘤学 | 110篇 |
出版年
2024年 | 2篇 |
2023年 | 20篇 |
2022年 | 55篇 |
2021年 | 84篇 |
2020年 | 53篇 |
2019年 | 64篇 |
2018年 | 84篇 |
2017年 | 57篇 |
2016年 | 65篇 |
2015年 | 56篇 |
2014年 | 82篇 |
2013年 | 108篇 |
2012年 | 118篇 |
2011年 | 134篇 |
2010年 | 86篇 |
2009年 | 68篇 |
2008年 | 75篇 |
2007年 | 76篇 |
2006年 | 80篇 |
2005年 | 70篇 |
2004年 | 81篇 |
2003年 | 62篇 |
2002年 | 49篇 |
2001年 | 17篇 |
2000年 | 14篇 |
1999年 | 16篇 |
1998年 | 7篇 |
1997年 | 8篇 |
1996年 | 8篇 |
1995年 | 8篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 3篇 |
1991年 | 8篇 |
1990年 | 8篇 |
1989年 | 6篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1980年 | 2篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1971年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有1774条查询结果,搜索用时 9 毫秒
11.
Triplett Noah S. Woodard Grace S. AlRasheed Rashed Meza Rosemary D. Robinson Sophia Berliner Lucy Dorsey Shannon 《The journal of behavioral health services & research》2021,48(4):617-624
The Journal of Behavioral Health Services & Research - Characterizing community mental health (CMH) treatment duration and discharge is an important step toward understanding how to better meet... 相似文献
12.
Joseane C. de Castro Laila V. de Almeida Mariana Santos Cardoso Fabricio M. Silva Oliveira Denise S. Nogueira João Luis Reis-Cunha Luisa M.D. Magalhaes Bin Zhan Maria Elena Bottazzi Peter J. Hotez Lilian L. Bueno Daniella Castanheira Bartholomeu Ricardo T. Fujiwara 《Vaccine》2021,39(2):394-401
An estimated 400 million people are infected by parasites of the genus Ascaris and the existing control measures are inefficient. Vaccine development using B cell antigens is a promising strategy for increased protection against this parasite. The present study aimed at developing a chimeric protein capable of conferring protection against infection by Ascaris sp. For this purpose, we performed B-cell epitope predictions on previously described vaccine candidate proteins from Ascaris suum and the corresponding peptides were used to construct a chimeric protein. Female BALB / c mice were immunized subcutaneously in three doses at 10 day intervals with a vaccine formulation comprised of the chimeric protein together with monophosphoryl lipid A (MPLA). Control groups included protein alone, MPLA, or PBS. After challenge infection, animals vaccinated with chimeric protein plus MPLA showed a reduction of 73.54% of larval load in the lung compared to control group animals. Animals immunized with chimeric protein plus MPLA also display higher IgG response and a reduction in lung inflammation. Our study highlights how chimeric proteins containing more than one B cell epitope can enhance immune protection against helminthic infection and offer new approaches to the development of Ascaris vaccines. 相似文献
13.
An oxidimetric titrant, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone in anhydrous acetic acid is used for the semimicro-determination
of hydrazine hydrate, phenylhydrazine hydrochloride, isoniazid and iproniazid phosphate in pure forms as well as in some pharmaceutical
preparations containing isoniazid and iproniazid phosphate. The end point was detected potentiometrically using a platinum-calomel
combination electrode. The results obtained are compared statistically with those obtained by the official methods and they
are in good agreement. 相似文献
14.
P G Pearson J G Slatter M S Rashed D H Han T A Baillie 《Chemical research in toxicology》1991,4(4):436-444
The reactivity toward peptides and proteins of S-(N-methylcarbamoyl)glutathione (SMG), the glutathione conjugate of methyl isocyanate, and the corresponding cysteine adduct, S-(N-methylcarbamoyl)cysteine (SMC), was investigated with the aid of in vitro model systems. Incubation of SMC or a trideuteriomethyl analogue of SMC with either the reduced or oxidized forms of oxytocin afforded similar mixtures of mono-, bis- and tris-N-methylcarbamoylated peptides. Structure elucidation of the mono and bis adducts by fast atom bombardment tandem mass spectrometry indicated that carbamoylation of oxytocin occurred preferentially at Cys-6 and that Cys-1 and/or Tyr-2 were secondary sites of modification. Upon incubation of S-[N-([14C]methyl)carbamoyl]glutathione (14C-SMG) with native bovine serum albumin (BSA), radioactivity became bound covalently to the protein in a time- and concentration-dependent fashion. "Blocking" of the lone Cys-34 thiol group of BSA in the form of a disulfide prior to exposure of the protein to 14C-SMG failed to decrease significantly the extent or time course of this covalent binding. It is concluded that carbamate thioester conjugates of MIC are reactive, carbamoylating entities which can donate the elements of MIC to nucleophilic functionalities on peptides and proteins. Free thiols appear to be preferred sites for such carbamoylation processes, a phenomenon that may have important toxicological consequences in the pathology of tissue lesions induced by MIC and related isocyanates. 相似文献
15.
Cancer is a leading cause of death worldwide and affects society in terms of the number of lives lost. Current cancer treatments are based on conventional chemotherapy which is nonspecific in targeting cancer. Therefore, intensive efforts are underway to better target cancer-specific cells while minimizing the side effects on healthy tissues by using LDL particles as active drug delivery vehicles. The goal is to encapsulate anticancer agents thiosemicarbazone metal-ligand complexes into LDL particles to increase the cytotoxic effect of the agent by internalization through LDL receptors into MCF7, A549, and C42 cancer cell lines as segregate models for biological evaluations targeting tubulin. Zeta potential data of LDL-particles encapsulated anticancer agents showed an acceptable diameter range between 66–91 nm and uniform particle morphology. The results showed cell proliferation reduction in all tested cell lines. The IC50 values of LDL encapsulated thiosemicarbazone metal-ligand complexes treated with MCF7, A549, and C42 ranged between 1.18–6.61 µM, 1.17–9.66 µM, and 1.01–6.62 µM, respectively. Western blot analysis showed a potent decrease in tubulin expression when the cell lines were treated with LDL particles encapsulated with thiosemicarbazone metal-ligand complexes as anticancer agents. In conclusion, the data provide strong evidence that LDL particles are used as an active drug delivery strategy for cancer therapy. 相似文献
16.
17.
Compliance with hand hygiene practices is directly affected by the accessibility and availability of cleaning agents. Nevertheless, the decision of where to locate these dispensers is often not explicitly or fully addressed in the literature. In this paper, we study the problem of selecting the locations to install alcohol-based hand sanitizer dispensers throughout a hospital unit as an indirect approach to maximize compliance with hand hygiene practices. We investigate the relevant criteria in selecting dispenser locations that promote hand hygiene compliance, propose metrics for the evaluation of various location configurations, and formulate a dispenser location optimization model that systematically incorporates such criteria. A complete methodology to collect data and obtain the model parameters is described. We illustrate the proposed approach using data from a general care unit at a collaborating hospital. A cost analysis was performed to study the trade-offs between usability and cost. The proposed methodology can help in evaluating the current location configuration, determining the need for change, and establishing the best possible configuration. It can be adapted to incorporate alternative metrics, tailored to different institutions and updated as needed with new internal policies or safety regulation. 相似文献
18.
This urban community clinic had poor asthma outcomes compared to the state of Minnesota. The standard of care supports an annual Asthma Action Plan (AAP). However, the majority of patients at this clinic had not received one. This quality improvement project aimed to improve asthma control, measured by an asthma control test of at least 20, through AAP implementation supported by all team members. The clinic’s interdisciplinary champion group implemented workflow changes via practice facilitation and Plan-Do-Study-Act cycles. Asthma control rates increased from 23% to 58% in adults and 45% to 63% in children over one year, a statistically significant change. 相似文献
19.