The immunostatus of natural killer cells in people exposed to sulfur mustard

Sulfur mustard (2,2-dichloroethyl sulfide, SM) has been documented as an alkylating agent. It has been widely used as a chemical weapon during the last two decades. Despite extensive worldwide research, no effective therapy has yet been devised for the treatment of patients exposed to SM. A severe suppression of the immune system still remains as the major cause of opportunistic infections, septicemia and death in such patients. The aim of this study was to determine the possible effect of SM on natural killer (NK) cells in patients suffering from SM injuries. Patients were classified into three groups: mild, moderate and severe. Blood sample obtained from each patient was examined using flowcytometric technique. Results showed that the percentage of NK cells (CD45+/CD56+) is significantly lower in severe patients than that of the control group (P<0.05). It was also observed that the activity of NK cells (CD56+/CD25+) in severe alkylating group is noticeably higher compared with the control group (P<0.1).     

Both combined oral azithromycin plus allopurinol and intramuscular Glucantime yield low efficacy in the treatment of Old World cutaneous leishmaniasis: a randomized controlled clinical trial

Old World cutaneous leishmaniasis (OWCL) is an endemic and major health problem in Iran. The optimal treatment of OWCL is unknown, and current treatments are not ideally effective and have many adverse effects. To compare the efficacy and tolerability of combined oral azithromycin and allopurinol with intramuscular Glucantime in the treatment of OWCL, we conducted a prospective randomized clinical trial. A total of 86 patients with OWCL were assigned and divided randomly into two groups; they received a combination of azithromycin capsule 10 mg/kg/d and allopurinol tablet 10 mg/kg/d for two months or IM injection of Glucantime 20 mg/kg of antimony daily for 20 days. All patients were followed for two months after termination of treatment. Although immediately at the end of the treatment period, complete response was seen in 27.8% of patients on combination therapy vs. 0% in the Glucantime group. The combination of azithromycin and allopurinol had a better outcome; two months after the end of the treatment period, complete, partial, and no responses were seen in 38.9%, 22.2%, and 38.9% in combination therapy and 40%, 31.4%, and 28.6% in the Glucantime group. There was no significant difference between the response rate in both groups after two months (P = 0.5). No severe adverse effect occurred. This study demonstrated that the efficacy of combined oral azithromycin and allopurinol at the above doses and duration was similar to that of IM Glucantime in the treatment of OWCL.     

Fourth-generation fluoroquinolone-resistant mycobacterial keratitis after laser in situ keratomileusis

We report a case of mycobacterial keratitis resistant to fourth-generation fluoroquinolones after laser in situ keratomileusis (LASIK) with fourth-generation fluoroquinolone prophylaxis. While receiving moxifloxacin post LASIK, the patient was diagnosed with moxifloxacin-resistant Mycobacterium chelonae keratitis. Culture susceptibilities revealed isolates resistant to moxifloxacin and gatifloxacin, and treatment with topical amikacin and clarithromycin with oral doxycycline and clarithromycin along with flap amputation was necessary to control the infection. This case demonstrates the potential limitations in the coverage of these antibiotic agents.     

The modulating effects of Resveratrol on the expression of MMP-2 and MMP-9 in endometriosis women: a randomized exploratory trial

Endometriosis is an inflammatory disease; the hallmark of inflammation is over-activation of matrix metalloproteinases (MMPs). The regulatory effects of Resveratrol on MMPs were formerly depicted in other cell lines. This study aimed at investigating the effects of Resveratrol on expression of MMP-2 and -9 in endometriosis patients. This trial was carried out on endometriosis patients (n?=?34) who were randomly divided into treatment (i?=?17) and control (n?=?17) groups. Alongside the routine protocol, the control and treatment groups took placebo and Resveratrol (400?mg), respectively, for 12–14?weeks. Endometrial tissue and fluid as well as blood sampling from both groups were done before and after the intervention. The level of mRNA and protein of both MMP-2 and -9 reduced in the endometrium of treatment group following intervention. Also, the serum and the endometrial fluid concentration of them lowered within the treatment group. Moreover, the serum and endometrial fluid levels of MMP-2 as well as MMP-9 were also diminished following the surgical removal of endometritic lesions. We showed that Resveratrol can modify the inflammation process in the endometrium of women with endometriosis at least in the level of MMP-2 and -9 expressions. The therapeutic potency of Resveratrol in endometriosis needs more clinical studies.     

The effectiveness of acceptance and commitment therapy on mental health and mindfulness in mothers of newborns with cystic fibrosis

BackgroundThe birth of a child is always a beautiful experience for any family. With the birth of a sick baby, however, all wishes will be lost. The existence of a sick newborn, the loss of wishes, and the hardships, along with financial pressures, may have adverse effects on the family's mental health, especially the mother. In this regard, acceptance and commitment therapy can help mothers get rid of the thought that the newborn's illness will hurt his/her future.MethodsThe present study is quasi-experimental research in the pretest and posttest form that was performed with control groups and a one-month follow-up. Random sampling was used to recruit thirty mothers of newborn children with cystic fibrosis disorder under the auspices of the Iranian Pediatric Hospital. Subjects responded to the SCL-90 questionnaire and the Five Facet Mindfulness Questionnaire (FFMQ) before and after the intervention, and one month later in the follow-up period. Acceptance and commitment therapy intervention was administered to the experimental group in 8 sessions (60–90 min each) for three weeks. The control group did not receive any interventions. Repeated measures analyses of variance (ANOVA) was used to analyze the data.ResultsThe results showed significant differences between the control and experimental groups in all facets of mindfulness toward the expected non-judgemental attitude and the mother's acceptance (p < 0.5).ConclusionIt can be concluded that commitment-based therapy was effective. Therefore, this method can be used to raise the mindfulness and health of the mother having a newborn with cystic fibrosis.     

Demonstration of the mineralocorticoid hormone receptor and action in human leukemic cell lines.

We studied the expression of the mineralocorticoid receptor (MCR), and of the amiloride-sensitive sodium channel (ASSC) regulated by the MCR, in human leukemic cell lines. Cell extracts from TF1 (proerythroblastic), HEL (human erythroblastic leukemia) and U937 (myeloblastic) cell line were positive for the ASSC, as a 82 kDa band in Western blots developed with the aid of a polyclonal antibody raised against the peptide QGLGKGDKREEQGL, corresponding to the region 44-58 of the alpha subunit of the epithelial sodium channel (ENaC) cloned from rat colon, linked to KLH. The polyclonal antibody against the MCR revealed a single band of about 102 kDa in extracts from HEL and TF1 cells. The immunofluorescent labelling of the MCR in all cell lines showed a nucleocytoplasmic localization of the receptor but the ASSC was exclusively membrane-bound and these results were confirmed by confocal microscopy. The expression of the MCR in the HEL cells was evident as a predicted band of 843 bp (234 amino acids) in electrophoresis of the PCR product obtained after total RNA had been reverse transcribed and then amplified using the primers 5'-AGGCTACCACAGTCTCCCTG-3' and 5'-GCAGTGTAAAATCTCCAGTC-3' (sense and antisense, respectively). The ENaC was similarly evident with the aid of the primers 5'-CTGCCmATG GATGATGGT-3' (sense) and 5'-GTTCAGCTCGAAGAAGA-3' (antisense) as a predicted band of 520 bp. In both cases, 100% identity was observed between the sequences of the PCR products compared to those from known human sources. The multiplication of the HEL cells was influenced by antagonists (RU 26752, ZK 91587) targeted for specificity to the MCR and this was selectively reversed by the natural hormone aldosterone. These steroids also provoked chromatin condensation in the HEL population. These permit new and novel possibilities to understand the pathobiology of human leukemia and to delineate sodium-water homeostasis in nonepithelial cells.     

Psychometric properties of the Iranian version of the Sexual Modes Questionnaire (SMQ): to assess the association between automatic thoughts,emotions and sexual response

The role of cognitive-affective factors on male and female sexual functioning has been studied in North America and European countries and there is a lack of validated measures available for use elsewhere. The aim of this study was to translate and validate the Sexual Modes Questionnaire (SMQ) to the Iranian context. The SMQ is a validated measure aimed at assessing the association between thoughts, emotions and sexual response during sexual activity. A total of 388 participants (224 women and 164 men) completed the SMQ. A principal component analysis with varimax rotation and subsequent confirmatory factor analysis (CFA) were performed. Three factors were identified in the male version: Failure Anticipation Thoughts, Erection Concern Thoughts, and Lack of Erotic Thoughts. In the female version, six factors were identified: Failure and Disengagement Thoughts, Low Self-Body Image Thoughts, Sexual Abuse Thoughts, Sexual Passivity and Control, Lack of Erotic Thoughts, and Partner's Lack of Affection. Also, a consistent pattern of correlations was found between the three subscales of the SMQ (thoughts, emotions, and sexual response) supporting the interactional character of the measure. This study has provided some preliminary evidence of the reliability and validity of the SMQ for use in Iranian populations.