Brief Note: Plastic Tube Technic of Centrifugal Separation of Leukocytes from Human Blood

A new simple plastic tube centrifugation method is described for the fractional harvesting of viable leukocytes from 10 ml. of human blood.

Submitted on June 8, 1961 Accepted on July 13, 1961     

RING CHROMOSOME 14 IN A MENTALLY RETARDED GIRL

Abstract. Iselius, L., Ritzen, M., Hui, T.-H., Olsson, K. and Eklöf, O. (Departments of Clinical Genetics, Pediatrics, Ophthalmology and Pediatric Radiology, Karolinska Hospital, Stockholm, Sweden). Ring chromosome 14 in a mentally retarded girl. Acta Paediatr Scand, 69:803, 1980.—A case of ring chromosome 14 in a 12-year-old girl, showing mental retardation, epilepsy and minor somatic abnormalities, is described and compared with five previously reported cases with the same chromosome aberration.     

Sealing properties of intermediary bases and effect on rat molar pulp

abstract — Five "cream-like" intermediary bases (Hypocal®, Dycal®, Reocap®, Cavitec® and Dropsin®) were studied using ZnO-eugenol (ZOE) and phosphate cement for comparison. The in vitro experiments, which were designed to reveal the penetration of a dye and an etching liquid, showed that Dropsin and Hypocal were permeable to dye and that Dropsin was permeable to acid as well. The in vivo experiments revealed no definite differences between the different cream-like base materials, nor were there differences between those and the conventional cements. The overall mildness of the dentin-pulp reactions and the suitability of the rat molar for this kind of research are discussed.     

A QUANTITATIVE EVALUATION OF THE EFFECT OF ONYTERACYCLINE AND DOXYCYCLINE IN ACNE VULGARIS

SUMMARY.— A double-blind cross over study on the effect of oxytetracycline and doxycycline in acne vulgaris was performed on 28 patients. The acne lesions were classified in different grades of severity and counted. In this way a reasonably precise assessment of the clinical picture could be obtained. After 4 weeks of treatment with either 750 mg. of oxytetracycline or 100 mg. of doxycycline daily, the severity of the disease was reduced to about 55% of the initial values. After reduction of the dose to one-third of the initial dose, a relapse was noted in 8 patients, 6 of whom had been treated with 100 mg- of doxycycline every third day. On average, improvement continued during the 12 weeks of treatment. At this time the "total acne load" was reduced to 19–31% of the initial values (corresponding to 28–39% of the total number of lesions). No definite side effects were noted.     

Active Basal Immunity and Its Application to Epidemiology III. Combined Immunization against Diphtheria, Tetanus, and Pertussis in Infancy

Summary
Some practical aspects of simultaneous immunization of infants against diphtheria, tetanus, and pertussis by means of mixed (triple) vaccines were investigated. Three injections were made, at 3, 4¹/₂ and 6 months of age. Mild febrile reactions occurred in 22 per cent of the infants. Local reactions were common, particularly when a vaccine with a stronger pertussis component was employed. Three different sites of inoculation (back, arm, and leg) were compared; preference is given to the supraspinous fossa since persistent infiltration with consequent risk of abscess was less common there. The antibody response to diphtheria and tetanus was very satisfactory. In regard to pertussis, vaccine I (45 billion pertussis bacilli per 3 cc) gave rather low agglutinin titres, whereas vaccine II (60–75 billions per 3 cc) evoked a more satisfactory response.