全文获取类型
收费全文 | 159篇 |
免费 | 8篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 17篇 |
妇产科学 | 3篇 |
基础医学 | 19篇 |
口腔科学 | 4篇 |
临床医学 | 23篇 |
内科学 | 31篇 |
皮肤病学 | 2篇 |
神经病学 | 1篇 |
特种医学 | 32篇 |
外科学 | 5篇 |
综合类 | 5篇 |
预防医学 | 7篇 |
眼科学 | 1篇 |
药学 | 14篇 |
中国医学 | 1篇 |
肿瘤学 | 10篇 |
出版年
2021年 | 1篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2014年 | 1篇 |
2013年 | 5篇 |
2012年 | 3篇 |
2011年 | 2篇 |
2010年 | 5篇 |
2009年 | 9篇 |
2008年 | 4篇 |
2007年 | 9篇 |
2006年 | 4篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2002年 | 8篇 |
2001年 | 4篇 |
2000年 | 9篇 |
1999年 | 3篇 |
1998年 | 12篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 10篇 |
1993年 | 10篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 7篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1875年 | 1篇 |
排序方式: 共有177条查询结果,搜索用时 15 毫秒
41.
Autologous bone marrow transplantation is potentially curative in the treatment of acute leukemia if residual leukemic cells in the marrow can be eliminated prior to transplantation. We studied the purging effects of a synthetic alkyl-lysophospholipid (ALP) on marrow containing leukemic cells from a transplantable myelomonocytic leukemia (WEHI-3B) in BALB/c mice. Simulated remission bone marrow containing 2% leukemic cells treated in vitro with 20 and 100 micrograms/mL of ET-18- OCH3 (1-octadecyl-2-methyl-sn-glycerol-3-phosphocholine) significantly prolonged survival of lethally irradiated transplanted recipients. At a dose of 100 micrograms/mL, 88% of the mice survived for the duration of the experiment (approximately five months). Autopsies showed that 25% of these survivors had microscopic evidence of leukemia. Thus, in vitro treatment of marrow eliminated leukemic blasts and spared sufficient normal stem cells to allow hematologic reconstitution. The effect of ET- 18-OCH3 is not entirely selective for leukemic cells. A spleen colony assay showed that ALP has some cytotoxic effect on normal hematopoietic stem cells. 相似文献
42.
Resident bone marrow macrophages (RBMM) play an important role in clearance of nuclei extruded from late-stage erythroblasts (Eb). To investigate the nature of macrophage receptors involved in this process, extruded erythroblast nuclei (EEN) were purified by cultivation of erythroblasts with erythropoietin, followed by density gradient centrifugation. By electron microscopy, the majority of free nuclei had an intact plasma membrane. EEN bound avidly to RBMM in a divalent cation-independent manner at both 4 degrees C and 37 degrees C. The use of specific monoclonal antibodies (MoAbs) and inhibitors showed that this adhesive interaction was not mediated by previously characterized macrophage receptors involved in recognition of either developing hematopoietic cells or apoptotic cells. The EEN receptor was expressed on resident macrophages isolated from murine bone marrow, spleen, lymph node, and peritoneal cavity, but was completely absent from alveolar macrophages. Despite high levels of EEN binding to RBMM, few were phagocytosed even after prolonged culture. Phorbol myristate acetate (PMA) was found to stimulate phagocytosis, suggesting that this is a regulated process. These findings indicate that EEN are recognized by a novel macrophage receptor and that recognition may be triggered during the membrane remodeling that accompanies enucleation. 相似文献
43.
Combination cytokine therapy continues to be evaluated in an effort to stimulate multilineage hematopoietic reconstitution after bone marrow myelosuppression. This study evaluated the efficacy of combination therapy with the synthetic interleukin-3 receptor agonist, Synthokine- SC55494, and recombinant methionyl human granulocyte colony-stimulating factor (rhG-CSF) on platelet and neutrophil recovery in nonhuman primates exposed to total body 700 cGy 60Co gamma radiation. After irradiation on day (d) 0, cohorts of animals subcutaneously received single-agent protocols of either human serum albumin (HSA; every day [QD], 15 micrograms/kg/d, n = 10), Synthokine (twice daily [BID], 100, micrograms/kg/d, n = 15), rhG-CSF (QD, 10 micrograms/kg/d, n = 5), or a combination of Synthokine and rhG-CSF (BID, 100 and 10 micrograms/kg/d, respectively, n = 5) for 23 days beginning on d1. Complete blood counts were monitored for 60 days postirradiation and the durations of neutropenia (absolute neutrophil count < 500/microL) and thrombocytopenia (platelet count < 20,000/microL) were assessed. Animals were provided clinical support in the form of antibiotics, fresh irradiated whole blood, and fluids. All cytokine protocols significantly (P < .05) reduced the duration thrombocytopenia versus the HSA-treated animals. Only the combination protocol of Synthokine + rhG-CSF and rhG-CSF alone significantly shortened the period neutropenia (P < .05). The combined Synthokine/rhG-CSF protocol significantly improved platelet nadir versus Synthokine alone and HSA controls and neutrophil nadir versus rhG-CSF alone and HSA controls. All cytokine protocols decreased the time to recovery to preirradiation neutrophil and platelet values. The Synthokine/rhG-CSF protocol also reduced the transfusion requirements per treatment group to 0 among 5 animals as compared with 2 among 5 animals for Synthokine alone, 8 among 5 animals for rhG-CSF, and 17 among 10 animals for HSA. These data showed that the combination of Synthokine, SC-55494, and rhG-CSF further decreased the cytopenic periods and nadirs for both platelets and neutrophils relative to Synthokine and rhG-CSF monotherapy and suggest that this combination therapy would be effective against both neutropenia and thrombocytopenia consequent to drug- or radiation- induced myelosuppression. 相似文献
44.
Rasool O; Heyman M; Brandter LB; Liu Y; Grander D; Soderhall S; Einhorn S 《Blood》1995,85(12):3431-3436
Malignant cells from 52 children with acute lymphocytic leukemia (ALL) were investigated for inactivation of the p15ink4B and p16ink4 genes and other genetic alterations on chromosome 9p21. Homozygous deletions of the p15ink4B and/or the p16ink4 genes were detected in 16 cases and a further 9 cases showed evidence of allelic loss either by hemizygous deletion or loss of heterozygosity (LOH) for 9p21 markers. Most cases had loss of both genes, but 5 patients had lost only p16ink4 and 2 cases had homozygous loss of p15ink4B only. Sequence analysis of all exons of p15ink4B and p16ink4 was performed in patients with hemizygous deletions or LOH for 9p21 markers. A frame shift mutation of p16ink4 exon 1 was shown in 1 case, whereas all other clones carried the wild- type sequence of p15ink4B and p16ink4 in the remaining allele. The data suggest that both the p15ink4B and p16ink4 genes can be inactivated in ALL. The existence of a hitherto undefined tumor-suppressor gene on chromosome 9p cannot be ruled out. 相似文献
45.
Elliott MJ; Vadas MA; Eglinton JM; Park LS; To LB; Cleland LG; Clark SC; Lopez AF 《Blood》1989,74(7):2349-2359
Two human hemopoietic growth factors involved in monocytopoiesis, interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were studied for their ability to stimulate blood monocytes and to bind to the monocyte membrane. Both cytokines maintained monocyte/macrophage numbers during long-term culture and increased cell size as compared with controls. Effects on cell numbers were present at low cytokine concentrations (6 to 20 pmol/L), whereas enhanced 3H-thymidine incorporation was observed only at higher concentrations (greater than or equal to 60 pmol/L). Autoradiographic studies showed only 1% to 3% of stimulated monocytes with nuclear grains. These results suggest that the primary mechanism for IL-3 and GM-CSF-induced maintenance of monocyte/macrophage numbers in humans is through an effect on cell survival. Surface receptors for both IL-3 and GM-CSF were studied by using 125I-labeled recombinant human (rh) cytokines and performing Scatchard analyses. Both cytokines showed curvilinear Scatchard plots, and computer analyses favored a two-site binding model. High-affinity binding data for 125I rhIL-3 (Kd 7.7 to 38.2 pmol/L; receptor number/cell 95 to 580) and for 125I rhGM-CSF (Kd 4.7 to 38.9 pmol/L; receptor number/cell 8 to 67) show similar binding affinities for the two cytokines but a lower receptor number/cell for 125I rhGM-CSF. Low-affinity binding characteristics for 125I rhIL-3 (Kd 513 to 939 pmol/L; receptor number/cell 179 to 5,274) and for 125I rhGM- CSF (Kd 576 to 1,120 pmol/L; receptor number/cell 130 to 657) show a similar pattern for the two cytokines. Specificity of 125I rhIL-3 and 125I rhGM-CSF binding to monocytes was established by the ability of the homologous cytokine to inhibit binding and the inability of a range of other cytokines to compete at 100-fold excess molar concentration. It is important, however, that binding of 125I rhIL-3 was partially inhibited by rhGM-CSF and that rhIL-3 partially inhibited binding of 125I rhGM-CSF to the monocyte membrane under conditions shown to prevent receptor internalization. The degree of inhibition varied between 25% and 80% in different experiments, and quantitative inhibition experiments showed that 1,000-fold excess concentrations of competitor failed to inhibit binding of the heterologous ligand completely. These results demonstrate that human IL-3 and GM-CSF have similar effects on growth and survival of human monocytes in vitro and suggest that these and other common biological effects may be mediated either through a common receptor or through distinct receptors associated on the monocyte membrane. 相似文献
46.
47.
Schwartz LB; Chiu AS; Courtney M; Krey L; Schmidt-Sarosi C 《Human reproduction (Oxford, England)》1997,12(1):45-50
To evaluate embryonic and endometrial factors for their value in predicting
pregnancy outcome in in-vitro fertilization (IVF) and embryo transfer, a
retrospective data collection and prospective uterine artery colour Doppler
imaging study was performed in a university-based IVF-embryo transfer
programme setting. A total of 210 patients were included and grouped as
follows: (I) IVF with controlled ovarian stimulation (214 cycles); (II)
frozen-thaw cycle of autologous embryos (30 cycles); (III) oocyte donation,
no cryopreservation (12 cycles); (IV) frozen-thaw cycle with embryos from
donated oocytes (10 cycles). Embryo quality was significantly better in
pregnant than non-pregnant cycles (group I, P = 0.0104; groups II-IV, P =
0.0418). The endometrial echo was significantly thicker in pregnant versus
non-pregnant patients in group I (P = 0.0059), but not in groups II-IV (P =
0.741). Past uterine surgery or abnormalities had no effect on pregnancy
outcome. There were no significant differences in mean uterine artery
resistance index or peak systolic velocity in pregnant versus non-pregnant
patients in groups II-IV. Thus, embryo quality is the most reliable
predictor of pregnancy outcome. Endometrial measurements were significantly
thicker in subsequently pregnant patients only in group I, where the
endometrium reflects the hormonal environment. Doppler parameters were not
useful in predicting pregnancy outcome.
相似文献
48.
Lawrence ��LB�� Brown 《Journal of the National Medical Association》2004,96(12):1679-1679
49.
口腔诊所信息管理系统的研究与开发 总被引:1,自引:0,他引:1
目的 研究利用计算机信息处理、数据库和图形技术 ,开发用于口腔诊所 (科室 )的信息管理系统。方法 在专家论证诊所 (科室 )的医疗现状和管理需求的基础上 ,制定我国口腔临床医疗操作代码 ,用PowerBuilder6 .0和SQLAnywhere数据库管理系统为开发工具 ;采用C语言作为图形开发工具 ,进行信息管理系统的研制。结果 该系统含 7个子系统 :患者信息管理、工作人员管理、财务管理、诊所资产管理、消耗品管理、技工委托、系统维护。经 8个单位试用 ,一年中累计管理患者约 110 0例。测试结果表明 ,系统具有较强和较完善的临床使用功能及方便的用户界面。结论 该系统能提高使用单位的临床医疗管理水平和效益。对提高医生的病历水平和积累研究资料也具有重要意义。 相似文献
50.