Studies of Human Erythrocyte Catalase by Fluorescent Antibody Technic The Distribution of Catalase in Acatalasia and Hypocatalasia

In order to investigate the cellular distribution of catalase in normal,hypocatalasic and acatalasic red blood cells, the fluorescent antibody labellingtechnic was employed. Sensitive anticatalase sera were produced in rabbitsby immunization with purified catalase extracted from human erythrocytes.Specificity against human erythrocyte catalase was confirmed by Ouchterlony’sdouble diffusion method.

The distribution of catalase is fairly homogeneous in normal and hypocatalasic red cells, but in acatalasic cells fluorescence due to the presence ofcatalase was not observed.

By this method the amount of catalase in hypocatalasic red cells was judgedto be between that of normal and acatalasic red cells.

Submitted on October 1, 1968 Accepted on February 13, 1969     

Preliminary Phase II Study of Adriamycin (ADM) in Patients with Non-Small Cell Lung Cancer (NSCLC)

A phase II study of adriamycin (ADM) (60 mg/m² was performedin 22 patients with non-small cell lung carcinoma (NSCLC). Therewere no responders in the 19 evaluable patients (16 with adenocarcinoma,two with squamous cell carcinoma and one with large cell carcinoma).The major side effects were alopecia (89%), leukocytopenia (73%),thrombocytopenia (58%) and upper gastrointestinal symptoms. Although ADM at 60 mg/m² did not appear to have sufficient antitumoractivity against NSCLC in this study, it is necessary to evaluatefurther the efficacy of ADM against NSCLC with another treatmentschedule.     

Chitosan-DNA nanoparticles: the effect of cell type and hydrolysis of chitosan on in vitro DNA transfection

Commercial chitosan (Ch) with low (LMWCh) and medium molecular weight (MMWCh) were hydrolyzed in diluted hydrochloric acid by heating at different temperatures. The viscosity average molecular weight of Chs was gradually decreased from 450 to 14 kDa as a function of temperature. Ch fractions were used for formation of Ch-DNA nanoparticles and tested for the ability to introduce DNA into HEK293, Swiss3T3, HeLa, and MDCK cells in vitro. The average diameter of nanoparticles was 200-220 nm. The surface charge of nanoparticles varied depending on the Ch/DNA ratio. The cell lines different response to DNA transfection with Ch fractions depended on molecular weight. HEK293 cells were efficiently transfected by nanoparticles prepared with Chs having a wide range of molecular weight (approximately 14-195 kDa). Swiss3T3 cells were efficiently transfected by Ch polymers with about <17 kDa. In contrast, HeLa and MDCK cells were highly resistant to DNA transfection with Ch polymers. These results strongly suggest that Ch polymers may be widely used for DNA trasnfection of the mammalian cells under optimized conditions.     

Developmental Alteration in RNA Metabolism of El Mouse Brain

Abstract: The contents of brain RNA rose rapidly until 14 days after birth and then dropped. They were lower in the El than in the non-convulsive mice between 56 and 100 days of life. The contents of acid-soluble UMP and their specific radioactivities, three hours after an intracranial injection of [³H]-orotic acid, were not different in the same age of El and non-convulsive mouse brains. From four to 20 days after birth, the specific and relative specific radioactivities, representing the ratio of brain RNA synthesis, of the El mice were lower than those of the non-convulsive strains, while the El mice, applying convulsions once a week, exhibited higher values during the periods from 56 to 100 days of age as compared with the non-stimulated El or non-convulsive strains.     

Anti-inflammatory effects of perioperative intensive insulin therapy during cardiac surgery with cardiopulmonary bypass

Purpose  

Recent studies have reported that controlling blood glucose with insulin can suppress systemic inflammation. In the present study, we evaluated how perioperative intensive insulin therapy (IIT) influences the inflammatory response in an artificial pancreas during cardiac surgery with cardiopulmonary bypass.