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941.
Ju-yeon Choi Eun-Jin Kim Hyun Joo Rho Jin Young Kim Oh-Kyung Kwon Jin-Hee Lee Min Jee Koo Sung Soon Kim 《Journal of virological methods》2009,161(1):7-11
Human immunodeficiency virus type-1 (HIV-1) RNA viral load is an important biomarker to evaluate the therapeutic efficacy of antiretroviral drugs and to monitor disease progression in HIV-infected individuals. We compared HIV-1 RNA quantitation between two different kits, the NucliSens EasyQ® HIV-1 v1.1 (EasyQ, bioMérieux) and RealTime HIV-1 (RealTime, Abbott), using HIV-1 RNA quality control (QC) materials, cell-cultivated viruses, and the plasma samples of 104 patients with HIV. Correlation between the two kits for HIV RNA-1 quantitation with clinical samples was high (R = 0.91). Based on results obtained with quality control standards, the reproducibility of the RealTime kit was higher than the EasyQ kit: the viral load value and coefficient of variation of each kit was 4.11 ± 0.136 and 3.3% for EasyQ and 3.55 ± 0.042 and 1.2% for RealTime, respectively (P < 0.002).This is the first comparative analysis of the detection limit and reproducibility of two different quantitation kits using clinical plasma samples from Korean HIV-1-infected patients. It will serve a useful reference to determine correction values for each HIV-1 RNA quantitation kits and to choose an appropriate assay kit for each laboratory. 相似文献
942.
Scott Ryan Green Richard S. Kwon Grace H. Elta Yogesh B. Gianchandani 《Biomedical microdevices》2010,12(3):477-484
This paper presents the in situ and ex vivo evaluation of a system that wirelessly monitors the accumulation of intimal tissue and sludge in a biliary stent. The sensing
element, located within the stent, is a magnetoelastic resonator that is queried by a wireless radio frequency signal. The
in situ testing uses a commercially-available self-expanding biliary stent enhanced with a 1 mm × 25 mm magnetoelastic ribbon sensor
(formed from Metglas™ 2605SA1). The stent has a conformal magnetic layer (consisting of strontium ferrite particles suspended
in polydimethylsiloxane) that biases the sensor. The external interrogation module is able to acquire a signal from the sensor
from a distance of at least 5 cm while the sensor is implanted in a porcine carcass and loaded with biological fluids. The
ex vivo testing uses bile harvested from the porcine carcass. The response of a 1 mm × 25 mm magnetoelastic ribbon sensor is first
calibrated with fluids of known density and viscosity, and the calibrated sensor is used to estimate that the viscosity of
the harvested bile is 2.7–3.7 cP. The test results presented in this paper illustrate the fundamental usability of the system
when the sensor is implanted, loaded by biological fluids, and interrogated in a surgical setup. 相似文献
943.
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945.
Uyenchi N Le Hyung-Seok Kim Jin-Sook Kwon Mi Yeon Kim Vu H Nguyen Sheng Nan Jiang Byeong-Il Lee Yeongjin Hong Myung Geun Shin Joon Haeng Rhee Hee-Seung Bom Youngkeun Ahn Sanjiv S Gambhir Hyon E Choy Jung-Joon Min 《Molecular therapy》2011,19(5):951-959
Optimization of the specific affinity of cardiac delivery vector could significantly improve the efficiency of gene/protein delivery, yet no cardiac vectors to date have sufficient target specificity for myocardial infarction (MI). In this study, we explored bacterial tropism for infarcted myocardium based on our previous observations that certain bacteria are capable of targeting the hypoxic regions in solid tumors. Out of several Escherichia coli or Salmonella typhimurium strains, the S. typhimurium defective in the synthesis of ppGpp (ΔppGpp S. typhimurium) revealed accumulation and selective proliferation in the infarcted myocardium without spillover to noncardiac tissue. The Salmonellae that were engineered to express a variant of Renilla luciferase gene (RLuc8), under the control of the E. coli arabinose operon promoter (PBAD), selectively targeted and delivered RLuc8 in the infarcted myocardium only upon injection of -arabinose. An examination of the infarct size before and after infection, and estimations of C-reactive protein (CRP) and procalcitonin indicated that intravenous injection of ΔppGpp S. typhimurium did not induce serious local or systemic immune reactions. This current proof-of-principle study demonstrates for the first time the capacity of Salmonellae to target infarcted myocardium and to serve as a vehicle for the selective delivery of therapeutic agents in MI. 相似文献
946.
The efficacy and safety of a monophasic hyaluronic acid filler in the correction of nasolabial folds: A randomized,multicenter, single blinded,split‐face study 下载免费PDF全文
947.
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949.
Soon Ae Lee Hye Jung Kim Ki Churl Chang Jong Chul Baek Ji Kwon Park Jeong Kyu Shin Won Jun Choi Jong Hak Lee Won Young Paik 《The Korean journal of physiology & pharmacology》2009,13(4):301-307
Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioprotective potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin E2 (PGE2) and IL-6 production, and NF-κB activity were measured by Western blot, ELISA, and luciferase activity, respectively. Results showed that EPA, DHA, or troglitazone significantly reduced COX-2 expression, NF-κB luciferase activity, and PGE2 and IL-6 production in a dose-dependent fashion. Interestingly, low doses (10 µM) of DHA and EPA, but not troglitozone, significantly increased the activity of NF-κB in resting HUVECs. Our study suggests that while DHA, EPA, and troglitazone may be protective on HUVECs under inflammatory conditions in a dose-dependent manner. However there may be some negative effects when the concentrations are abnormally low, even in normal endothelium. 相似文献