全文获取类型
收费全文 | 925篇 |
免费 | 27篇 |
国内免费 | 2篇 |
专业分类
儿科学 | 8篇 |
妇产科学 | 8篇 |
基础医学 | 135篇 |
口腔科学 | 3篇 |
临床医学 | 44篇 |
内科学 | 254篇 |
皮肤病学 | 7篇 |
神经病学 | 61篇 |
特种医学 | 13篇 |
外科学 | 171篇 |
综合类 | 2篇 |
预防医学 | 15篇 |
眼科学 | 8篇 |
药学 | 101篇 |
中国医学 | 1篇 |
肿瘤学 | 123篇 |
出版年
2023年 | 5篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 10篇 |
2016年 | 15篇 |
2015年 | 10篇 |
2014年 | 7篇 |
2013年 | 18篇 |
2012年 | 31篇 |
2011年 | 29篇 |
2010年 | 33篇 |
2009年 | 21篇 |
2008年 | 39篇 |
2007年 | 44篇 |
2006年 | 58篇 |
2005年 | 63篇 |
2004年 | 77篇 |
2003年 | 97篇 |
2002年 | 95篇 |
2001年 | 24篇 |
2000年 | 24篇 |
1999年 | 25篇 |
1998年 | 30篇 |
1997年 | 17篇 |
1996年 | 13篇 |
1995年 | 18篇 |
1994年 | 8篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 10篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 12篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 6篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1955年 | 1篇 |
排序方式: 共有954条查询结果,搜索用时 9 毫秒
11.
The female patient was diagnosed as having Von Gierke's disease at 14 years of age, based on clinical manifestations, laboratory examination and liver biopsy. At 19 years of age she had uremia and died from its deterioration at 24 years of age. The parents were consanguineous, and a 27-year-old sister is presently hospitalized for renal Insufficiency with hepatomegaly. On autopsy, the patient's kidneys were highly contracted and contained a number of small cysts, mainly in the medulla. Histological examination indicated periglomerular fibrosis, glomerular hyalinization, tubular atrophy or cystic dilatation and intersitial fibrosis with round cell infiltration. These findings correspond to Fanconi's familial juvenile nephronophthlsis, except for age. The liver was markedly enlarged and Indicated severe glycogen deposits, but the kidney did not contain glycogen deposits. It can, therefore, be presumed that the renal lesions were not a secondary consequence of long-term glycogen deposits but that renal and hepatic lesions were associated with each other. 相似文献
12.
Aoyama K Ozaki Y Nakanishi T Ogasawara MS Ikuta K Aoki K Blomgren K Suzumori K 《American journal of reproductive immunology (New York, N.Y. : 1989)》2004,52(6):362-369
PROBLEM: The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated. METHOD OF STUDY: Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis. RESULTS: The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia. CONCLUSIONS: Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium. 相似文献
13.
Koga T Hashimoto S Sugio K Yonemitsu Y Nakashima Y Yoshino I Matsuo Y Mojtahedzadeh S Sugimachi K Sueishi K 《American journal of clinical pathology》2002,117(3):464-470
We assessed the occurrence of atypical adenomatous hyperplasia (AAH) in whole lung lobes with primary cancer lesions. Following surgical resection, tissue specimens were sliced to a thickness of 4 mm (3,641 specimens from 61 cases; mean = 59.7 specimens per case). A total of 119 AAH foci were found and an association was evident in 25 (57%) of 44 adenocarcinomas, 3 (30%) of 10 squamous cell carcinomas, and 2 (29%) of 7 other lung cancers. Histologic evaluation showed that 108 AAH foci were categorized as low-grade and the other 11 as high-grade AAH. These 11 foci of high-grade AAH were present in 7 patients with adenocarcinoma, and in 1 patient there was a synchronous double primary lung adenocarcinoma. High-grade AAH was closely associated with bronchioloalveolar carcinoma (BAC) type adenocarcinoma, and low-grade AAH with non-BAC adenocarcinoma. The mean +/- SD Ki-67 labeling index in high-grade AAH (3.5%+/-2.9%) was significantly higher than for the low-grade index (1.4%+/-1.6%). We propose that foci of high- but not low-grade AAH may be potential precursor lesions of lung adenocarcinoma, especially with the BAC component. 相似文献
14.
Role of apoptosis controlled by cytochrome c released from mitochondria for luteal function in human granulosa cells 总被引:1,自引:0,他引:1
Makino A Ozaki Y Matsubara H Sato T Ikuta K Nishizawa Y Suzumori K 《American journal of reproductive immunology (New York, N.Y. : 1989)》2005,53(3):144-152
PROBLEM: The aim of this study was to investigate the relationship between apoptosis by the mitochondrial pathway and luteal function in human granulosa cells. METHOD OF STUDY: Granulosa cells were obtained by ultrasound-guided follicular aspiration from patients undergoing in vitro fertilization and embryo transfer. After the addition of RU486, cells were stained with a mitochondria-specific fluorescent dye, MitoTracker Red CM x Ros. Using flow cytometry and National Institute of Health image, the mitochondrial fluorescent area was measured. After staining with Hoechst 33258 dye, the number of apoptotic bodies per 1000 cells were counted at random on photomicrographs. Homogenates were used for sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis using antibodies against cytochrome c or caspase-3. RESULTS: The incidence of apoptotic bodies increased and the mitochondrial membrane potential decreased time dependently. The opposite effect was observed dose dependently with RU486 treatment. Western blot analysis showed increased cytochrome c expression, after treatment with 1-2 microg/mL of RU486 which then decreased with 5-10 microg/mL of RU486. Caspase-3 expression increased dose dependently with RU486. CONCLUSIONS: These results suggest that the activation of caspase-3 caused by cytochrome c released from mitochondria plays an important role in apoptosis-related luteal function in human granulosa cells. 相似文献
15.
Ichiro Yamadori Makoto Motoi Kazuhiko Hayashi Akira Tsutsumi Katsuo Ogawa Kenji Doi Kazuo Hamaya 《Pathology international》1984,34(2):459-469
Two cases of disseminated infection caused by Mycobacterium intracellulare were reported and discussed. In the first case, the patient was a fifty-seven-year-old male who complained of general fatigue, weight loss, and fever. Biopsy of the right inguinal lymph nodes and the liver revealed infiltration by histiocytes engulfing many acid-fast bacilli. At autopsy an egg-sized abscess was found is the region of the right Iliac lymph nodes. Histological examination showed histiocytic infiltration in the abscess wall, neighboring lymph nodes, liver, and spleen. In the second case, the patient was a four-year-old boy, who had persistent fever and splenomegaly. Splenectomy was performed and histological examination of the spleen revealed multiple nodular infiltration by swollen histiocytes with many acid-fast bacilli in their cytoplasm. The bone marrow aspirates and liver tissue obtained in the necropsy also showed many histiocytes containing many acid-fast bacilli. The authors emphasized the importance of paying special attention to atypical mycobacteriosis in feverish patients having lesions with a proliferation of histiocytes. 相似文献
16.
Wakamatsu T Saito T Hayashi J Takeichi T Kitamoto K Aizawa K 《Medical molecular morphology》2005,38(4):225-232
Flexible treatments for intimal hyperplasia after angioplasty are still needed. The aim of this study was to demonstrate the
long-term effects of vascular photodynamic therapy with talaporfin sodium on intimal hyperplasia following interventional
injury. Intimal hyperplasia was induced by balloon distension injury to the carotid artery in 31 rabbits. Talaporfin, 5.0 mg/kg,
was delivered systemically immediately after balloon injury. The injury site was irradiated with a diode laser light of wavelength
664 nm using a fluence of 50 J/cm2 after 30 min. At day 3 and weeks 3, 6, 9, 15, and 25 after photodynamic therapy, the treated artery of each rabbit was excised
and examined immunohistochemically. Thirty minutes after talaporfin administration, drug fluorescence was found only in the
balloon-injured carotid artery wall. At 3 days, no smooth muscle cells were seen in the media of the photodynamic therapy-treated
arterial segments. Intimal hyperplasia developed progressively in the balloon-injured and untreated segments; however, in
the segments treated with photodynamic therapy, intimal hyperplasia was markedly suppressed until 25 weeks and the media was
repopulated by smooth muscle cells without macrophages. Vascular photodynamic therapy with talaporfin may be used to inhibit
restenosis after vascular intervention.
An erratum to this article is available at . 相似文献
17.
We investigated changes in the P100 latency of the visual evoked potential (VEP) and the saccadic reaction time (SRT) in relation to the degree of activity of the shoulder girdle elevators. Muscle force was set in 10% increments from 0% to 50% of the maximal voluntary contraction (MVC). The VEP was derived from a midline occipital electrode with reference electrodes on the ears when the right retina was stimulated through the eyelid by light emitting diodes while the eyes were closed. The P100 latency of the VEP was defined as the time from the stimulus onset to the main positive peak. The SRT was defined as the latency until the beginning of eye movement toward the lateral target, which was moved at random time-intervals. P100 latency was shortened until 30% of the MVC, and which it lengthened. The SRT changed in a pattern similar to that observed for the P100 latency. The ratio of the shortening in P100 latency relative to that of the SRT was approximately 20%. All data is presented as the mean value, plus the standard deviation. We believe that the information processing time in the neural pathway from the retina to the visual cortex was shortened up to a certain muscle force of the shoulder girdle elevators, and then this processing time lengthened. These findings indicate that shortening of information processing time in the neural pathway beyond the visual cortex is included in the shortening of the SRT. 相似文献
18.
Expression of matrix Gla protein and osteonectin mRNA by human aortic smooth muscle cells. 总被引:3,自引:0,他引:3
Hiroyuki Hao Seiichi Hirota Hatsue Ishibashi-Ueda Toshio Kushiro Katsuo Kanmatsuse Chikao Yutani 《Cardiovascular pathology》2004,13(4):195-202
BACKGROUND: Recent data indicate that matrix proteins such as matrix Gla protein (MGP) and osteonectin (ON) influence not only mineralization of vasculature but smooth muscle cell (SMC) differentiation. METHODS: We examined whether MGP and ON are expressed by human aortic SMCs in vivo using Northern blotting, in situ hybridization and immunohistochemistry. RESULTS: MGP and ON mRNAs were strongly expressed in the aorta without atherosclerosis from newborn and four young subjects up to 10 years old. In the aorta from 15 adult cases, MGP and ON mRNAs were decreased as atherosclerosis developed. We determined cell type and distribution of the MGP- and ON mRNA-expressing cells by in situ hybridization and immunohistochemistry. In the aorta obtained from newborn and young subjects, SMCs in the media and thin intima expressed MGP mRNA and, to a lesser extent, ON mRNA. In the adult aorta with fibrous thickening, MGP mRNA was expressed by intimal SMCs and subpopulation of medial SMCs. Osteonectin mRNA was expressed mainly by intimal SMCs and few medial SMCs. Double immunohistochemical staining revealed that both MGP- and ON protein-expressing cells were positive for anti-alpha-smooth muscle actin antibody, aortic SMCs. CONCLUSIONS: These results suggested that MGP and ON expression by aortic SMCs might be regulated by the degree of atherosclerosis and SMC differentiation in human aorta. 相似文献
19.
20.
Iwabuchi Satoru Handa Masashi Usuda Katsuo Sato Masami Kondo Takashi Tanita Tatsuo Fujimura Shigefumi 《Surgery today》1994,24(11):1014-1018
A 52-year-old Japanese man with a slow-growing chondroma originating from the sternal bone was referred to our hospital. A subtotal resection of the sternum was performed, hereafter termed the sandwich method, and an originally designed prosthesis made from ultra-high-molecular-weight polyethylene and Marlex mesh was used for reconstruction. The postoperative course was uneventful without any symptoms due to paradoxical movement of the chest or regional abscess, and no disturbance in the movement of the upper limbs, such as a surgical sequelae, was observed. 相似文献