全文获取类型
收费全文 | 2991篇 |
免费 | 191篇 |
国内免费 | 40篇 |
专业分类
耳鼻咽喉 | 16篇 |
儿科学 | 38篇 |
妇产科学 | 26篇 |
基础医学 | 385篇 |
口腔科学 | 72篇 |
临床医学 | 200篇 |
内科学 | 868篇 |
皮肤病学 | 35篇 |
神经病学 | 151篇 |
特种医学 | 112篇 |
外科学 | 549篇 |
综合类 | 9篇 |
预防医学 | 107篇 |
眼科学 | 55篇 |
药学 | 229篇 |
中国医学 | 11篇 |
肿瘤学 | 359篇 |
出版年
2024年 | 8篇 |
2023年 | 56篇 |
2022年 | 101篇 |
2021年 | 176篇 |
2020年 | 95篇 |
2019年 | 86篇 |
2018年 | 133篇 |
2017年 | 78篇 |
2016年 | 106篇 |
2015年 | 96篇 |
2014年 | 103篇 |
2013年 | 143篇 |
2012年 | 205篇 |
2011年 | 233篇 |
2010年 | 121篇 |
2009年 | 100篇 |
2008年 | 148篇 |
2007年 | 164篇 |
2006年 | 161篇 |
2005年 | 165篇 |
2004年 | 124篇 |
2003年 | 126篇 |
2002年 | 119篇 |
2001年 | 33篇 |
2000年 | 34篇 |
1999年 | 42篇 |
1998年 | 37篇 |
1997年 | 25篇 |
1996年 | 25篇 |
1995年 | 11篇 |
1994年 | 13篇 |
1993年 | 7篇 |
1992年 | 18篇 |
1991年 | 7篇 |
1990年 | 15篇 |
1989年 | 14篇 |
1988年 | 4篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1973年 | 6篇 |
1971年 | 4篇 |
1970年 | 5篇 |
1966年 | 3篇 |
1965年 | 4篇 |
排序方式: 共有3222条查询结果,搜索用时 109 毫秒
31.
Kazuki Hayasaka Yui Watanabe Takashi Hirama Hisashi Oishi Masafumi Noda Hiroaki Toyama Yutaka Ejima Yoshikatsu Saiki Yoshinori Okada 《Transplantation proceedings》2021,53(4):1385-1387
Although single-lung transplant on the side with better lung function is challenging in patients with significantly asymmetrical lung function between the right and left sides, it sometimes can be a realistic option because of the recipient's condition and from the viewpoint of organ sharing. We report our experience with a successful case of single-lung transplant on the side with a pulmonary perfusion ratio of 89%. The transplant was performed with the patient under central venoarterial extracorporeal membrane oxygenation through a clamshell incision, and the patient had an acceptable short- and long-term outcome with a remarkable improvement of lung function. 相似文献
32.
I. Sano Y. Kakimoto K. Taniguchi M. Takesada 《Journal of molecular medicine (Berlin, Germany)》1960,38(1):41-45
Ohne Zusammenfassung 相似文献
33.
Masahiko Igarashi Yuki Takeda Seijiro Mori Naoko Ishibashi Eiichi Komatsu Kentaro Takahashi Tsunekazu Fuse Mikako Yamamura Kazuki Kubo Yasuo Sugiyama Yasushi Saito 《British journal of pharmacology》1997,120(6):1172-1178
- The aim of this study was to determine whether BAYw6228 (BAYw), a newly developed 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitor, could suppress an atherogenic process such as intimal thickening by a mechanism other than lowering the level of serum cholesterol.
- First, we evaluated the in vitro effect of BAYw on the proliferation of vascular smooth muscle cells (SMC) from various species: Sprague-Dawley (SD) rats, New Zealand (NZ) white rabbits, intimal cells from Watanabe hereditary hyperlipidemic (WHHL) rabbit and SMC from the new-born human aorta. The increasing rate of total protein content of these cells was inhibited by the addition of BAYw in a dose-dependent fashion. In the presence of 2% foetal calf serum (FCS), the value of IC50 was 1.0 μM in SD rats. 2.1 μM in NZ white rabbits, and 0.3 μM in WHHL rabbits. With human SMC, the value was 0.02 μM in the presence of 10% FCS and 0.2 μM with a mixture of growth factors.
- Based on these above in vitro findings, we next examined the in vivo effect of the agent to determine whether it could suppress rabbit intimal thickening induced by balloon catheterization. A balloon catheter was inserted from a peripheral branch of the left external carotid artery to the aorta to denude the endothelium of the left common carotid artery in Japanese white rabbits. After 12 days they were divided into control and BAYw groups. The former were subcutaneously injected with saline and the latter with BAYw 1 mg kg−1 day−1. Two days after the beginning of treatment, a second balloon injury was performed to the previously injured left common carotid artery in both groups. After another two weeks, the left common carotid artery was removed and variously stained. Although the total serum cholesterol in the BAYw group was significantly lower than in the control (P<0.05), the difference was not enough to affect intimal thickening. In addition, the BAYw group had a smaller intima/media ratio than the control group, decreasing to 45% of control (P<0.05). By anti-α smooth muscle actin antibody staining, these intimal thickening areas were entirely occupied by SMCs, and their amount was attenuated by BAYw. By anti-rabbit macrophage antibody (RAM 11) staining, the number of positive cells in the intimal thickening was markedly decreased in the BAYw group compared to control (P<0.01).
- These results indicate that BAYw has an inhibitory effect on intimal thickening by attenuating intimal SMC proliferation and infiltration of macrophages, suggesting that BAYw could be effective in the prevention of the progression of atherosclerotic plaque-like restenosis after angioplasty.
34.
PURPOSE: The aim of the study is to describe a case of suspected endotoxin-induced uveitis associated with septic endogenous endophthalmitis followed by antibiotic-induced endotoxemia. METHODS: The human leukocyte antigen (HLA) typing of peripheral leukocytes was studied by lymphocytotoxicity technique. Histological and immunohistochemical studies of paraffin embedded specimen were conducted. RESULTS: Findings of HLA typing revealed positive reaction for B 51, Cw 3, DR 8, DR 11, DQ 3. The vitreous body of an eviscerated eye was occupied by the non-specific granulomatous tissue, composed of fibroblast, plasma cells, and Sudan black staining positive foamy cells, including melaniferous phagocytes, identified as CD 68 positive macrophage. CONCLUSION: It is suggested that antibiotic-induced endotoxemia of a patient with septic endogenous endophthalmitis produced endotoxin-induced uveitis under an upregulation of HLA and endotoxin activated macrophages may release cytokines, followed by fibrin formation and subsequent granuloma. 相似文献
35.
Hydroxyamino, nitroso and nitro derivatives of 3-amino-1-methyl-5H-pyrido[4,3-b]indole(Trp-P-2) and 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole(Glu-P-1), mutagens-carcinogens produced on pyrolysis of aminoacids, were synthesized from Trp-P-2 and Glu-P-1. 3-Hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole(N-OH-Trp-P-2) and 2-hydroxyamino-6-methyldipyrido[l,2-a:3',2'-d]imidazole (N-OH-Glu-P-1) were obtained with good yieldsby controlled catalytic reduction of 3-nitro-l-methyl-5H-pyrido[4,3-b]indoleand 2-nitro-6-methyldipyrido[1,2-a:3',2'-d]imidazole. Subsequentoxidation of N-OH-Trp-P-2 and N-OH-Glu-P-1 with -manganese dioxideyielded 3-nitroso-1-methyl-5H-pyrido[4,3-b]indole and 2-nitroso-6-methyldipyrido[1,2-a:3',2'-d]imidazole.All six synthesized compounds were mutagenic to Salmonella typhimuriumTA98 without mammalian activation systems. The mutagenic activitiesof hydroxyamino and nitroso derivatives were identical for bothS. typhimurium TA98 and TA98NR, the nitroreductase deficientstrain. However, nitro derivatives were essentially mutageniconly towards S. typhimurium TA98. 相似文献
36.
37.
Daigo Murata Yoshio Endo Tohru Obata Kazuki Sakamoto Yasuhiro Syouji Masakazu Kadohira Akira Matsuda Takuma Sasaki 《Drug metabolism and disposition》2004,32(10):1178-1182
The antitumor 3'-ethynyl nucleosides, 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)cytosine (ECyd) and 1-(3-C-ethynyl-beta-D-ribopentofuranosyl)uridine (EUrd), are potent inhibitors of RNA polymerases and show excellent antitumor activity against various human solid tumors in xenograft models. ECyd is being investigated in phase I clinical trials as a novel anticancer drug possessing a unique antitumor action. ECyd and EUrd require the activity of uridine/cytidine kinase (UCK) to produce the corresponding active metabolite. The UCK family consists of two members, UCK1 and UCK2, and both UCKs are expressed in many tumor cells. It was unclear, however, whether UCK1 or UCK2 is responsible for the phosphorylation of the 3'-ethynyl nucleosides. We therefore established cell lines that are highly resistant to the 3'-ethynyl nucleosides from human fibrosarcoma HT-1080 and gastric carcinoma NUGC-3. All the resistant cell lines showed a high cross-resistance to ECyd and EUrd. As a result of cDNA sequence analysis, we found that UCK2 mRNA expressed in EUrd-resistant HT-1080 cells has a 98-base pair deletion of exon 5, whereas EUrd-resistant NUGC-3 cells were harboring the point mutation at nucleotide position 484 (C to T) within exon 4 of UCK2 mRNA. This mutation was confirmed by genome sequence analysis of the UCK2 gene. Moreover, the expression of UCK2 protein was decreased in these resistant cells. In contrast, no mutation in the mRNA or differences in protein expression levels of UCK1 were shown in the EUrd-resistant HT-1080 and NUGC-3 cells. These results suggest that UCK2 is responsible for the phosphorylation and activation of the antitumor 3'-ethynyl nucleosides. 相似文献
38.
39.
Aki Masuzawa Chikako Kiyotani Tomoo Osumi Yoko Shioda Kazutoshi Iijima Osamu Tomita Kazuhiko Nakabayashi Keisuke Oboki Kazuki Yasuda Hiromi Sakamoto Hitoshi Ichikawa Kenichiro Hata Teruhiko Yoshida Kenji Matsumoto Nobutaka Kiyokawa Tetsuya Mori 《European journal of haematology》2014,92(3):263-267
In addition to BCR, various rare fusion partners for the ABL1 gene have been reported in leukemia. We have identified the fusion gene SNX2‐ABL1 in a pediatric case of acute lymphoblastic leukemia (ALL), which has only once previously been reported in an adult patient. Cytogenetic analysis detected this fusion gene arising from a t(5;9)(q22;q34) translocation. ALL cells carrying a SNX2‐ABL1 fusion exhibited a BCR‐ABL1+ ALL‐like gene expression profile. The patient poorly responded to dasatinib but partially responded to imatinib. Treatment using tyrosine kinase inhibitors requires further investigation to optimize the genotype‐based treatment stratification for patients with SNX2‐ABL1 fusion. 相似文献
40.
Clinical impact of surveillance colonoscopy using magnification without diminutive polyp removal
下载免费PDF全文
![点击此处可从《Digestive endoscopy》网站下载免费的PDF全文](/ch/ext_images/free.gif)