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排序方式: 共有3140条查询结果,搜索用时 640 毫秒
81.
Fluorochrome‐based definition of naturally occurring Foxp3+ regulatory T cells of intra‐ and extrathymic origin 下载免费PDF全文
Cathleen Petzold Nadine Steinbronn Marcus Gereke Ruth H. Strasser Tim Sparwasser Dunja Bruder Robert Geffers Sonja Schallenberg Karsten Kretschmer 《European journal of immunology》2014,44(12):3632-3645
Under physiological conditions, studies on the biology of naturally induced Foxp3+ Treg cells of intra‐ and extrathymic origin have been hampered by the lack of unambiguous markers to discriminate the mature progeny of such developmental Treg‐cell sublineages. Here, we report on experiments in double‐transgenic mice, in which red fluorescent protein (RFP) is expressed in all Foxp3+ Treg cells, whereas Foxp3‐dependent GFP expression is exclusively confined to intrathymically induced Foxp3+ Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize naturally induced Treg cells of intrathymic (RFP+GFP+) and extrathymic (RFP+GFP?) origin in otherwise unmanipulated mice. These experiments directly demonstrate that extrathymically induced Treg cells substantially contribute to the overall pool of mature Foxp3+ Treg cells residing in peripheral lymphoid tissues of steady‐state mice. Furthermore, we provide evidence that intra‐ and extrathymically induced Foxp3+ Treg cells represent distinct phenotypic and functional sublineages. 相似文献
82.
Sabrina Trapp Karsten Mueller Jöran Lepsien Bernd Krämer Oliver Gruber 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》2014,232(2):619-628
Many studies have demonstrated attenuated verbal working memory (WM) under articulatory suppression. However, performance is not completely abolished, suggesting a less efficient, non-articulatory mechanism for the maintenance of verbal information. The neural causes for the reduced efficiency of such a putative complementary maintenance system have not yet been addressed. The present study was conducted to fill this gap. Subjects performed a Sternberg task (a) under articulatory maintenance at low, high, and supracapacity set sizes and (b) under non-articulatory maintenance at low and high set sizes. With functional magnetic resonance imaging, set-size related increases in activity were compared between subvocal articulatory rehearsal and non-articulatory maintenance. First, the results replicate previous findings showing different networks underlying these two maintenance strategies. Second, activation of all key nodes of the articulatory maintenance network increased with the amount of memorized information, showing no plateau at high set sizes. In contrast, for non-articulatory maintenance, there was evidence for a plateau at high set sizes in all relevant areas of the network. Third, for articulatory maintenance, the non-articulatory maintenance network was additionally recruited at supracapacity set sizes, presumably to assist processing in this highly demanding condition. This is the first demonstration of differential neural bottlenecks for articulatory and non-articulatory maintenance. This study adds to our understanding of the performance differences between these two strategies supporting verbal WM. 相似文献
83.
84.
Shengting Li Soren Besenbacher Yingrui Li Karsten Kristiansen Niels Grarup Anders Albrechtsen Thomas Spars? Thorfinn Korneliussen Torben Hansen Jun Wang Rasmus Nielsen Oluf Pedersen Lars Bolund Mikkel H Schierup 《European journal of human genetics : EJHG》2014,22(8):1040-1045
In this paper, we mine full mtDNA sequences from an exome capture data set of 2000 Danes, showing that it is possible to get high-quality full-genome sequences of the mitochondrion from this resource. The sample includes 1000 individuals with type 2 diabetes and 1000 controls. We characterise the variation found in the mtDNA sequence in Danes and relate the variation to diabetes risk as well as to several blood phenotypes of the controls but find no significant associations. We report 2025 polymorphisms, of which 393 have not been reported previously. These 393 mutations are both very rare and estimated to be caused by very recent mutations but individuals with type 2 diabetes do not possess more of these variants. Population genetics analysis using Bayesian skyline plot shows a recent history of rapid population growth in the Danish population in accordance with the fact that >40% of variable sites are observed as singletons. 相似文献
85.
Identification and functional characterization of imatinib‐sensitive DTD1‐PDGFRB and CCDC88C‐PDGFRB fusion genes in eosinophilia‐associated myeloid/lymphoid neoplasms 下载免费PDF全文
Darko Gosenca Beate Kellert Georgia Metzgeroth Claudia Haferlach Alice Fabarius Juliana Schwaab Michael Kneba Christof Scheid Karin Töpelt Philipp Erben Torsten Haferlach Nicholas C. P. Cross Wolf‐Karsten Hofmann Wolfgang Seifarth Andreas Reiter 《Genes, chromosomes & cancer》2014,53(5):411-421
Eosinophilia‐associated myeloid neoplasms with rearrangement of chromosome bands 5q31‐33 are frequently associated with PDGFRB fusion genes, which are exquisitely sensitive to treatment with imatinib. In search for novel fusion partners of PDGFRB, we analyzed three cases with translocation t(5;20)(q33;p11), t(5;14)(q33;q32), and t(5;17;14)(q33;q11;q32) by 5′‐rapid amplification of cDNA ends polymerase chain reaction (5′‐RACE‐PCR) and DNA‐based long‐distance inverse PCR (LDI‐PCR) with primers derived from PDGFRB. LDI‐PCR revealed a fusion between CCDC88C exon 25 and PDGFRB exon 11 in the case with t(5;17;14)(q33;q11;q32) while 5′‐RACE‐PCR identified fusions between CCDC88C exon 10 and PDGFRB exon 12 and between DTD1 exon 4 and PDGFRB exon 12 in the cases with t(5;14)(q33;q32) and t(5;20)(q33;p11), respectively. The PDGFRB tyrosine‐kinase domain is predicted to be retained in all three fusion proteins. The partner proteins contained coiled‐coil domains or other domains, which putatively lead to constitutive activation of the PDGFRB fusion protein. In vitro functional analyses confirmed transforming activity and imatinib‐sensitivity of the fusion proteins. All three patients achieved rapid and durable complete hematologic remissions on imatinib. © 2014 Wiley Periodicals, Inc. 相似文献
86.
A Short‐Read Multiplex Sequencing Method for Reliable,Cost‐Effective and High‐Throughput Genotyping in Large‐Scale Studies 下载免费PDF全文
Hongzhi Cao Yu Wang Wei Zhang Xianghua Chai Xiandong Zhang Shiping Chen Fan Yang Caifen Zhang Yulai Guo Ying Liu Zhoubiao Tang Caifen Chen Yaxin Xue Hefu Zhen Yinyin Xu Bin Rao Tao Liu Meiru Zhao Wenwei Zhang Yingrui Li Xiuqing Zhang Laurent C. A. M. Tellier Anders Krogh Karsten Kristiansen Jun Wang Jian Li 《Human mutation》2014,35(2):270-270
87.
Garofalo A Giai C Lattar S Gardella N Mollerach M Kahl BC Becker K Prince AS Sordelli DO Gómez MI 《The Journal of infectious diseases》2012,206(1):81-90
Staphylococcus aureus protein A (SpA) plays a critical role in the induction of inflammation. This study was aimed to determine whether the number of short sequence repeats (SSRs) present in the polymorphic region modulates the inflammatory response induced by SpA. We demonstrated that there is a dose-response effect in the activation of interferon (IFN)-β signaling in airway epithelial and immune cells, depending on the number of SSRs, which leads to differences in neutrophil recruitment. We also determined that a significant proportion of isolates from patients with chronic infections such as osteomyelitis and cystic fibrosis carry fewer SSRs than do isolates from patients with acute infections or healthy carriers and that there was an inverse correlation between the number of SSRs and the length of disease course. Given the importance of IFN signaling in eradication of S. aureus, loss of SSRs may represent an advantageous mechanism to adapt to and persist in the host. 相似文献
88.
Falkenberg LE Westerhausen R Specht K Hugdahl K 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(13):5069-5073
The dorsal anterior cingulate cortex (dACC) is a core structure for the governing of cognitive control, and recent studies have shown that interindividual differences in dACC anatomy are associated with corresponding differences in the ability for cognitive control. However, individuals differ not only in anatomical features of dACC, but also exhibit substantial variability regarding the biochemical characteristics of the dACC. In this study, we combined magnetic resonance spectroscopy ((1)H-MRS) and functional magnetic resonance imaging (fMRI), finding that interindividual differences of glutamate levels in the dACC during resting-state predict the strength of the blood-oxygen level-dependent (BOLD) response to a task requiring cognitive control. This relationship was observed in the retrosplenial cortex, the orbitofrontal cortex, the inferior parietal lobe, and the basal ganglia. More specifically, individuals with low resting-state glutamate levels in the dACC showed an increased BOLD response when the task demands were high, whereas high-glutamate individuals showed the opposite pattern of an increased BOLD response when the task demands were low. Thus, we show here that individual variability of glutamate levels is directly related to how the brain implements cognitive control. 相似文献
89.
Kröger C Dillon SC Cameron AD Papenfort K Sivasankaran SK Hokamp K Chao Y Sittka A Hébrard M Händler K Colgan A Leekitcharoenphon P Langridge GC Lohan AJ Loftus B Lucchini S Ussery DW Dorman CJ Thomson NR Vogel J Hinton JC 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(20):E1277-E1286
90.