全文获取类型
收费全文 | 3201篇 |
免费 | 156篇 |
国内免费 | 23篇 |
专业分类
耳鼻咽喉 | 32篇 |
儿科学 | 93篇 |
妇产科学 | 21篇 |
基础医学 | 351篇 |
口腔科学 | 87篇 |
临床医学 | 219篇 |
内科学 | 1039篇 |
皮肤病学 | 17篇 |
神经病学 | 257篇 |
特种医学 | 80篇 |
外科学 | 593篇 |
综合类 | 4篇 |
预防医学 | 67篇 |
眼科学 | 26篇 |
药学 | 180篇 |
中国医学 | 9篇 |
肿瘤学 | 305篇 |
出版年
2023年 | 33篇 |
2022年 | 74篇 |
2021年 | 129篇 |
2020年 | 70篇 |
2019年 | 72篇 |
2018年 | 90篇 |
2017年 | 74篇 |
2016年 | 83篇 |
2015年 | 64篇 |
2014年 | 107篇 |
2013年 | 122篇 |
2012年 | 178篇 |
2011年 | 212篇 |
2010年 | 96篇 |
2009年 | 83篇 |
2008年 | 154篇 |
2007年 | 167篇 |
2006年 | 139篇 |
2005年 | 165篇 |
2004年 | 160篇 |
2003年 | 149篇 |
2002年 | 183篇 |
2001年 | 59篇 |
2000年 | 54篇 |
1999年 | 49篇 |
1998年 | 56篇 |
1997年 | 40篇 |
1996年 | 28篇 |
1995年 | 27篇 |
1994年 | 20篇 |
1993年 | 22篇 |
1992年 | 40篇 |
1991年 | 38篇 |
1990年 | 31篇 |
1989年 | 39篇 |
1988年 | 28篇 |
1987年 | 28篇 |
1986年 | 27篇 |
1985年 | 18篇 |
1984年 | 17篇 |
1983年 | 18篇 |
1982年 | 10篇 |
1981年 | 13篇 |
1980年 | 16篇 |
1979年 | 9篇 |
1977年 | 10篇 |
1976年 | 10篇 |
1975年 | 8篇 |
1974年 | 11篇 |
1972年 | 9篇 |
排序方式: 共有3380条查询结果,搜索用时 655 毫秒
71.
Tomoko Takemoto Ryo Kaetsu Machiko Hanayama Yuuichi Ishiyama Masayuki Sadamura Kensuke Nishio Mariko Tsunoda Masatake Asano Mitsuru Motoyoshi 《International journal of medical sciences》2021,18(8):1746
The aim of this study was to examine the acid-electrolyzed functional water (FW)-mediated cytokine release in an oral squamous cell carcinoma-derived cell line (OSCC) following treatment with FW. FW is generated by the electrolysis of a sodium chloride solution and accelerate the burn wound healing. To elucidate the underlying mechanisms, the cytokine/chemokine secretion profile of HSC3 cells was examined using a cytokine array. FW treatment significantly induced interleukin (IL)-1α secretion, which was confirmed by enzyme-linked immunosorbent assay. Subsequently, the HSC3 cells were pre-treated with cycloheximide (CHX) for 1 h prior to FW stimulation to determine whether the augmented IL-1α secretion was due to enhanced protein synthesis. CHX pre-treatment did not affect IL-1α secretion suggesting that the secreted IL-1α might have been derived from intracellular storage sites. The amount of IL-1α in the cell lysate of the FW-treated HSC3 cells was significantly lower than that of the non-treated cells. Immunofluorescence staining using a polyclonal antibody against full-length IL-1α revealed a drastic reduction in IL-1α inside the FW- treated cells. IL-1α is synthesized in its precursor form (pIL-1α) and cleaved to produce pro-piece and mature IL-1α (ppIL-1α and mIL-1α) inside the cells. In the present study, only pIL-1α was detected within the HSC3 cells in its resting state. However, FW stimulation resulted in the release of the 33 kDa and two other smaller forms (about 19 kDa) of the protein. These results indicates that FW treatment induces IL-1α secretion, a typical alarmin, from the intracellular storage in OSCC cells. 相似文献
72.
Satoko Kojima Kei Kawana Kensuke Tomio Aki Yamashita Ayumi Taguchi Shiho Miura Katsuyuki Adachi Takeshi Nagamatsu Kazunori Nagasaka Yoko Matsumoto Takahide Arimoto Katsutoshi Oda Osamu Wada‐Hiraike Tetsu Yano Yuji Taketani Tomoyuki Fujii Danny J. Schust Shiro Kozuma 《American journal of reproductive immunology (New York, N.Y. : 1989)》2013,69(2):134-141
73.
Schimmel Martin Walther Moritz Al-Haj Husain Nadin Igarashi Kensuke Wittneben Julia Abou-Ayash Samir 《Clinical oral investigations》2021,25(11):6297-6307
Clinical Oral Investigations - To analyze the retention forces between primary and secondary telescopic crowns milled from various materials and to compare them with the retention forces between... 相似文献
74.
Nobuyuki Ashizawa Takahiro Takazono Kaname Ohyama Yoji Nagasaki Masaki Okamoto Tatsuro Hirayama Kensuke Takahashi Hirotomo Yamanashi Masato Tashiro Naoki Hosogaya Takeshi Tanaka Kazuko Yamamoto Yuichi Fukuda Yoshifumi Imamura Toshinori Kawanami Taiga Miyazaki Toyomitsu Sawai Kiyoyasu Fukushima Hiroshi Mukae 《Journal of infection and chemotherapy》2021,27(7):1033-1038
IntroductionNumerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serological tests exists commercially; however, their performance using clinical samples is limited. Although insufficient to detect SARS-CoV-2 in the early phase of infection, antibody assays can be of great use for surveillance studies or for some coronavirus disease 2019 (COVID-19) patients presenting late to the hospital.MethodsThis study evaluated the sensitivity and specificity of four commercial SARS-CoV-2 lateral flow antibody tests using 213 serum specimens from 90 PCR-positive confirmed COVID-19 patients. Of 59 negative control sera, 50 were obtained from patients with other respiratory infectious diseases before COVID-19 pandemic began while nine were from patients infected with other respiratory viruses, including two seasonal coronaviruses.ResultsThe varied sensitivities for the four commercial kits were 70.9%, 65.3%, 45.1%, and 65.7% for BioMedomics, Autobio Diagnostics, Genbody, and KURABO, respectively, between sick days 1 and 155 in COVID-19 patients. The sensitivities of the four tests gradually increased over time after infection before sick day 5 (15.0%, 12.5%, 15.0%, and 20.0%); from sick day 11–15 (95.7%, 87.2%, 53.2%, and 89.4%); and after sick day 20 (100%, 100%, 68.6%, and 96.1%), respectively. For severe illness, the sensitivities were quite high in the late phase after sick day 15. The specificities were over 96% for all four tests. No cross-reaction due to other pathogens, including seasonal coronaviruses, was observed.ConclusionsOur results demonstrated the large differences in the antibody test performances. This ought to be considered when performing surveillance analysis. 相似文献
75.
Kensuke Kaneko Kazushige Kawai Nelson H. Tsuno Soichiro Ishihara Hironori Yamaguchi Eiji Sunami Toshiaki Watanabe 《International surgery》2015,100(5):797-804
Allogeneic blood transfusion (ABT) has been reported as a major risk factor for surgical site infection (SSI) in patients undergoing colorectal surgery. However, the association of ABT with SSI in patients undergoing abdominoperineal resection (APR) and total pelvic exenteration (TPE) still remains to be evaluated. Here, we aim to elucidate this association. The medical records of all patients undergoing APR and TPE at our institution in the period between January 2000 and December 2012 were reviewed. Patients without SSI (no SSI group) were compared with patients who developed SSI (SSI group), in terms of clinicopathologic features, including ABT. In addition, data for 262 patients who underwent transabdominal rectal resection at our institution in the same period were also enrolled, and their data on differential leukocyte counts were evaluated. Multivariate analysis showed that intraoperative transfusion was an independent predictive factor for SSI after APR and TPE (P = 0.004). In addition, the first–operative day lymphocyte count of patients undergoing APR, TPE, and transabdominal rectal resection was significantly higher in nontransfusion patients compared with transfusion ones (P = 0.026). ABT in the perioperative period of APR and TPE may have an important immunomodulatory effect, leading to an increased incidence of SSI. This fact should be carefully considered, and efforts to avoid allogeneic blood exposure while still achieving adequate patient blood management would be very important for patients undergoing APR and TPE as well.Key words: Colorectal cancer, Abdominoperineal resection, Surgical site infection, Allogeneic blood transfusion, Patient blood managementPostoperative surgical site infection (SSI) is one of the most frequent complications associated with various surgical procedures, and it results in adverse outcomes, including longer hospital stay, higher health care costs, and increased surgical mortality.1 It is one of the most frequent nosocomial complications, accounting for almost one fifth of all health care–associated infections.2 Colon surgery and rectal surgery are associated with higher SSI rates compared with most other abdominal procedures, with 5% to 25% of colon and rectal surgery patients developing incisional and organ/space SSI.3–5 Moreover, the incidence of overall SSI was reported to be higher in rectal surgery patients (17%–28%) than in colonic surgery patients (9%–23%),3,5,6 with especially higher overall SSI rates observed in patients undergoing abdominoperineal resection (APR; 12%–51%).7–9 These are attributed to the high infection rates of the perineal wound, reported to be as high as 21%.10 Thus, the incidence of SSI associated with APR should be the highest among the various abdominal operative procedures.Various risk factors for postoperative SSI in colorectal surgery were reported previously. Open surgery,10–12 perioperative allogeneic blood transfusion (ABT),4,10,12 and prolonged operation time4,9 have been found to be risk factors for SSI in a number of studies. Although several preceding reports have investigated the risk factors for SSI associated with APR, the reported independent risk factors varied among the studies. Although a number of studies have reported on the role of ABT as a strong risk factor for incisional SSI in colorectal surgery,13,14 only one study has investigated on its relevance to the onset of incisional SSI after APR procedure; but this study failed to demonstrate a significant association. Presently, therefore, the role of ABT as a potential risk factor for incisional SSI in APR remains to be elucidated, and doing so will be very important for the implementation of measures to achieve patient blood management in this group of patients.In this study, we aimed to elucidate the risk factors for SSI in patients receiving APR, especially focusing on ABT. 相似文献
76.
77.
78.
Ishibashi M Hiasa K Zhao Q Inoue S Ohtani K Kitamoto S Tsuchihashi M Sugaya T Charo IF Kura S Tsuzuki T Ishibashi T Takeshita A Egashira K 《Circulation research》2004,94(9):1203-1210
Activated monocytes are present in the arterial walls of hypertensive patients and animals. Monocyte chemoattractant protein-1 (MCP-1), which controls monocyte function through its receptor (CCR2), is implicated in hypertensive inflammatory changes in the arterial wall. The role of CCR2 expression on monocytes in hypertension-induced vascular remodeling, however, has not been addressed. We hypothesized that CCR2 on monocytes is critical in hypertension-induced vascular inflammation and remodeling. Hypertension was induced by infusion of angiotensin II (Ang II) into wild-type mice, CCR2-deficient (CCR2-/-) mice, and bone marrow-transferred mice with a leukocyte-selective CCR2 deficiency (BMT-CCR2-/-). In wild-type mice, Ang II increased CCR2 intensity in circulating monocytes, which was prevented by an Ang II type-1 (AT1) receptor blocker or blunted in AT1 receptor-deficient mice. Enhanced CCR2 intensity on monocytes was observed in hypertensive patients and rats, and was reduced by treatment with the Ang II receptor blocker, supporting the clinical relevance of the observation in mice. In CCR2-/- and BMT-CCR2-/- mice, Ang II-induced vascular inflammation and vascular remodeling (aortic wall thickening and fibrosis) were blunted as compared with control mice. In contrast, Ang II-induced left ventricular hypertrophy developed in CCR2-/- and BMT-CCR2-/- mice. The present study suggests that CCR2 expression in monocytes has a critical role in vascular inflammation and remodeling in Ang II-induced hypertension, and possibly in other forms of hypertension. 相似文献
79.
Kensuke Kubota Yasunobu Abe Masahiko Inamori Harunobu Kawamura Hiroyuki Kirikoshi Noritoshi Kobayashi Satoru Saito Norio Ueno Atushi Nakajima 《Journal of hepato-biliary-pancreatic sciences》2005,12(4):286-289
Endoscopic gallbladder stenting is useful palliative therapy for acute cholecystitis in high‐risk patients. Although the success rate of endoscopic gallbladder stenting is 79%–100%, an alternative method has not been reported. We succeeded in employing a method for percutaneous gallbladder stenting (PTGS) and herein describe this new method. A patient with acute acalculous cholecystitis related to ischemic atherosclerotic vascular disease, cholangitis due to Lemmel syndrome, and severe congestive heart failure underwent PTGS through the cystic duct from the gallbladder to the duodenal papilla, because an endoscopic method failed in the treatment of Lemmel syndrome. Because we were unable to place endoscopic transpapillary gallbladder drainage, percutaneous transhepatic gallbladder drainage (PTGBD) was performed and both the cholecystitis and cholangitis ceased. PTGS was performed as an alternative to endoscopic gallbladder stenting. Access to the cystic duct and gallbladder was obtained by the PTGBD route, using a guidewire (0.035‐inch diameter) and seeking catheter (6.5 Fr) under fluoroscopic control. A 7‐Fr 12‐cm double‐pigtail biliary polyethylene stent was placed. The patient remained asymptomatic for 3 months after the PTGS until he died, of an acute recurrent myocardial infarction. This new PTGS placement is an alternative treatment for symptomatic gallbladder disease in patients with increased operative risk when the endoscopic method is unsuccessful. 相似文献
80.
Yokohama A Tsukamoto N Uchiumi H Handa H Matsushima T Karasawa M Murakami H Nojima Y 《Annals of hematology》2004,83(2):120-123
We describe a 65-year-old man diagnosed with Burkitt's lymphoma arising from the intestine. The tumor cells had a mature B-cell immunophenotype and rearrangement of the c-myc gene. The patient was treated with intensive multiagent chemotherapy. After four courses of chemotherapy, an ileus developed due to a residual abdominal disease. We administered rituximab in combination with the same chemotherapy regimen. A dramatic clinical improvement was observed and abnormal uptake by 18F-fluorodeoxyglucose positron emission tomography disappeared. The patient experienced complete remission for 1 year. This encouraging result indicates that rituximab might be an important treatment choice in management of Burkitt's lymphoma. 相似文献