全文获取类型
收费全文 | 5750篇 |
免费 | 340篇 |
国内免费 | 23篇 |
专业分类
耳鼻咽喉 | 35篇 |
儿科学 | 170篇 |
妇产科学 | 167篇 |
基础医学 | 674篇 |
口腔科学 | 85篇 |
临床医学 | 552篇 |
内科学 | 1260篇 |
皮肤病学 | 63篇 |
神经病学 | 384篇 |
特种医学 | 458篇 |
外科学 | 888篇 |
综合类 | 115篇 |
预防医学 | 361篇 |
眼科学 | 197篇 |
药学 | 432篇 |
1篇 | |
中国医学 | 27篇 |
肿瘤学 | 244篇 |
出版年
2022年 | 54篇 |
2021年 | 84篇 |
2020年 | 59篇 |
2019年 | 68篇 |
2018年 | 88篇 |
2017年 | 70篇 |
2016年 | 72篇 |
2015年 | 119篇 |
2014年 | 151篇 |
2013年 | 171篇 |
2012年 | 267篇 |
2011年 | 284篇 |
2010年 | 157篇 |
2009年 | 168篇 |
2008年 | 213篇 |
2007年 | 259篇 |
2006年 | 240篇 |
2005年 | 241篇 |
2004年 | 240篇 |
2003年 | 215篇 |
2002年 | 205篇 |
2001年 | 163篇 |
2000年 | 196篇 |
1999年 | 145篇 |
1998年 | 63篇 |
1997年 | 50篇 |
1996年 | 50篇 |
1995年 | 59篇 |
1994年 | 57篇 |
1993年 | 51篇 |
1992年 | 119篇 |
1991年 | 79篇 |
1990年 | 102篇 |
1989年 | 106篇 |
1988年 | 121篇 |
1987年 | 97篇 |
1986年 | 71篇 |
1985年 | 103篇 |
1984年 | 66篇 |
1983年 | 80篇 |
1981年 | 49篇 |
1979年 | 51篇 |
1978年 | 53篇 |
1977年 | 44篇 |
1975年 | 48篇 |
1974年 | 64篇 |
1972年 | 55篇 |
1971年 | 50篇 |
1970年 | 40篇 |
1967年 | 40篇 |
排序方式: 共有6113条查询结果,搜索用时 15 毫秒
51.
52.
Systematic isolation of transducing phages for Myxococcus xanthus. 总被引:25,自引:0,他引:25
Six new phages active on Myxococcus xanthus have been isolated from cultures of myxobacteria. The six are all capable of transduction, and they fall into three groups. Members of one group have long contractile tails, have a characteristic neutralization antigen, and resemble the previously described M×4. Members of a second group, exemplified by M×8, have very short tails and a characteristic antigen. M×9, the sole member of the third group, has a very short tail and a characteristic antigen. Phage M×8, which is active on fruiting as well as nonfruiting strains of M. xanthus, can transduce auxotrophic, antibiotic resistance and motility markers in M. xanthus. Although crude lysates of M×8 contain 58-nm diameter particles with a tail and 29-nm particles without tail, only 58-nm particles can form plaques and transduce. The plaque-forming particles of M×8 possess a single DNA molecule of 56,000 base pairs with a buoyant density of 1.726 g/cm3, virtually identical to that of the DNA from its host. 相似文献
53.
Summary Monensin, at concentrations which depended on the multiplicity of infection, was found to prevent DNA replication of human cytomegalovirus (HCMV) as well as production of viral progeny in human foreskin fibroblasts. The drug did not affect DNA replication of herpes simplex virus. Inhibition of consecutive HCMV DNA synthesis was also observed following delayed addition of the drug within 12–24 hours postinfection, but was fully reversible upon its removal. Viral replication proceeded, however, without impairment in cultures treated with monensin prior to infection. Induction of viral DNA polymerase activity was not impeded by the inhibitor. Analysis of protein- and glycoprotein synthesis revealed that monensin interfered with the production of a number of HCMV-specific polypeptides. Furthermore, evidence was obtained that the drug may hinder intracellular transport of a 135 kd glycopolypeptide.With 6 Figures 相似文献
54.
Cation channels,cell volume and the death of an erythrocyte 总被引:8,自引:0,他引:8
Lang F Lang KS Wieder T Myssina S Birka C Lang PA Kaiser S Kempe D Duranton C Huber SM 《Pflügers Archiv : European journal of physiology》2003,447(2):121-125
Similar to a variety of nucleated cells, human erythrocytes activate a non-selective cation channel upon osmotic cell shrinkage. Further stimuli of channel activation include oxidative stress, energy depletion and extracellular removal of Cl–. The channel is permeable to Ca2+ and opening of the channel increases cytosolic [Ca2+]. Intriguing evidence points to a role of this channel in the elimination of erythrocytes by apoptosis. Ca2+ entering through the cation channel stimulates a scramblase, leading to breakdown of cell membrane phosphatidylserine asymmetry, and stimulates Ca2+-sensitive K+ channels, thus leading to KCl loss and (further) cell shrinkage. The breakdown of phosphatidylserine asymmetry is evidenced by annexin binding, a typical feature of apoptotic cells. The effects of osmotic shock, oxidative stress and energy depletion on annexin binding are mimicked by the Ca2+ ionophore ionomycin (1 µM) and blunted in the nominal absence of extracellular Ca2+. Nevertheless, the residual annexin binding points to additional mechanisms involved in the triggering of the scramblase. The exposure of phosphatidylserine at the extracellular face of the cell membrane stimulates phagocytes to engulf the apoptotic erythrocytes. Thus, sustained activation of the cation channels eventually leads to clearance of affected erythrocytes from peripheral blood. Susceptibility to annexin binding is enhanced in several genetic disorders affecting erythrocyte function, such as thalassaemia, sickle-cell disease and glucose-6-phosphate dehydrogenase deficiency. The enhanced vulnerability presumably contributes to the shortened life span of the affected erythrocytes. Beyond their role in the limitation of erythrocyte survival, cation channels may contribute to the triggering of apoptosis in nucleated cells exposed to osmotic shock and/or oxidative stress. 相似文献
55.
J. M. Gourreau C. Kaiser M. Valette A. R. Douglas J. Labie M. Aymard 《Archives of virology》1994,135(3-4):365-382
Summary Samples collected in 1987 and 1988 in Brittany from influenzainfected swine made it possible to isolate and antigenically characterize two H1N2 recombinant viruses (Sw/France/5027/87 and Sw/France/5550/88). The former virus was cloned and reinoculated to swine to allow reproduction of the disease and reisolation of a strain similar to the original one. The serodiagnostic tests carried out on both the original sera and those from the experimentally infected animals confirmed that the virus was actually type Sw/H1N2. 相似文献
56.
Ohne Zusammenfassung 相似文献
57.
Bernd Kupfer Torsten Ruf Bertfried Matz Jacob Nattermann Ulrich Spengler Jürgen K Rockstroh Hans H Brackmann Johannes Blümel Michael Tacke Rolf Kaiser 《Journal of clinical virology》2005,34(1):42-47
BACKGROUND: Until the mandatory introduction of viral inactivation techniques of blood plasma products in the early 1980s many recipients of these products were infected with various viral pathogens. OBJECTIVES: To determine the rate of transmission of GB virus C/hepatitis G virus (GBV-C/HGV) HCV, and HIV through non-virus-inactivated clotting factor concentrates in hemophiliacs, as well as the relation between amount of administered clotting factor and risk for GBV-C/HGV infection. STUDY DESIGN: In this cross-sectional study, we determined retrospectively the rates of infection markers for GBV-C/HGV, HCV, and HIV in a German cohort of hemophiliacs treated with documented amounts of non-virus-inactivated clotting factor concentrates (group A) and in a second group of hemophiliacs who were treated exclusively with virus-inactivated clotting factor (group B). The presence of anti-virus antibodies was determined by ELISA. Viral RNA was detected by RT-PCR. Markers for viral infections were compared to amounts of administered non-virus-inactivated clotting factor. RESULTS: Among hemophiliacs treated with documented amounts of non-virus-inactivated clotting factor the prevalence for GBV-C/HGV, HCV, and HIV was 40.3%, 98.6%, and 56.3%, respectively. In contrast to HIV, the rate of GBV-C/HGV infections did not increase with increasing amounts of consumed non-inactivated clotting factor. Even in the subgroup of heavily treated hemophiliacs the rate of GBV-C/HGV infection markers did not exceed 45%. CONCLUSIONS: The amount of non-virus-inactivated clotting factor is not predictive for the risk of GBV-C/HGV infection in hemophiliacs. Despite repeated parenteral exposure more than 55% of hemophiliacs were not infected with GBV-C/HGV. Our findings indicate a high frequency of host factors preventing parenteral transmission of GBV-C/HGV. 相似文献
58.
Previously we have described the properties of store-operated channel currents (SOCs) in freshly dispersed rabbit portal vein smooth muscle cells. In addition to Ca2+ store depletion these SOCs could also be activated by α-adrenoceptor stimulation and diacylglycerol (DAG) via a protein kinase C (PKC)-dependent mechanism. In the present study we have investigated the effect of β-adrenoceptor stimulation on SOCs in rabbit portal vein myocytes. With whole-cell recording the selective β-adrenoceptor agonist isoprenaline reduced the current evoked by cyclopiazonic acid (CPA, sarcoplasmic/endoplasmic reticulum ATPase inhibitor) by over 85%. With cell-attached patch recording, bath application of isoprenaline produced a pronounced inhibition of SOC activity evoked by either CPA or the acetoxymethyl ester form of BAPTA (BAPTA-AM). SOC activity evoked by CPA, the DAG analogue, 1-oleoyl-acetyl- sn -glycerol (OAG) or the phorbol ester, phorbol-12,13-dibutyrate (PDBu) was also markedly inhibited by the adenylate cyclase activator, forskolin, and the cell-permeable non-hydrolysable analogue of cyclic adenosine monophosphate (cAMP), 8-Br-cAMP. With inside-out patches, bath application of PDBu evoked channel currents with similar properties to SOCs which were inhibited by over 90% by a catalytic subunit of protein kinase A (PKA) and by 8-Br-cAMP. Moreover bath application of PKA inhibitors, H-89, KT5720 and an inhibitory peptide to quiescent cell-attached or inside-out patches, activated channel currents with similar properties to SOCs. These data suggest that in rabbit portal vein myocytes, stimulation of β-adrenoceptors inhibits SOC activity via a cAMP-dependent protein kinase signal transduction cascade. In addition it is concluded that constitutive PKA activity has a profound inhibitory effect on SOC activity in this vascular preparation. 相似文献
59.
Paternally inherited deletion of CSH1 in a patient with Silver-Russell syndrome. 总被引:1,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Journal of medical genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
T Eggermann K Eggermann S Mergenthaler R Kuner P Kaiser M B Ranke H A Wollmann 《Journal of medical genetics》1998,35(9):784-786
In a continuing study on the aetiology of Silver-Russell syndrome (SRS), we detected a patient with a heterozygous deletion in the growth hormone gene cluster (17q22-q24). The deletion of the chorionic somatomammotrophin hormone 1 (CSH1) gene was inherited from the patient's father. The patient shows typical symptoms of SRS. Though deletions of CSH1 have been reported without any phenotypic consequences, the heterozygous deletion might be involved in the aetiology of SRS in the case presented here. Apart from other observations in SRS, like maternal uniparental disomy 7, changes in the genomic region 17q22-qter might be responsible for the expression of this syndrome for at least some of the patients, leading to the heterogeneity of SRS. 相似文献
60.
Schoch B Gorissen B Richter S Ozimek A Kaiser O Dimitrova A Regel JP Wieland R Hövel M Gizewski E Timmann D 《Journal of neurophysiology》2004,92(3):1856-1866
More recent findings suggest a possible role of the cerebellum in nonmotor functions. Disability of individuals with cerebellar damage in rapidly shifting attention is one frequently used example to support cerebellar involvement in mental skills. The original proposal was based on findings in five children with chronic surgical lesions of the cerebellum and a young adult with a degenerative disorder. The aim of the present study was to repeat Akshoomoff and Courchesne's initial findings in a larger group of children with focal cerebellar lesions. Ten children with cerebellar lesions and 10 age- and sex-matched controls were tested. Neocerebellar areas were affected in all children with cerebellar damage except one based on detailed analysis of MRI scans. Subjects had to perform a focus and a shift attention task. Two visual and two auditory stimuli were presented in a pseudorandom order. An ellipse and a high-pitched tone were presented less frequently than a circle and a low-pitched tone. Rare stimuli were presented at five different time intervals. In the focus tasks, subjects had to react to the same rare stimulus of one of the two modalities. In the shift task, subjects had to switch between the two rare stimuli. Motor deficits based on reaction times were small in cerebellar children compared with controls. The ability of target detection did not significantly differ in the children with cerebellar lesions compared with the control children in both the focus and the shift attention task. In particular, children with cerebellar damage showed no significant impairment in rapid (<2 s) shifts of attention. The present findings indicate that the cerebellum may be less critical in attention related processes than suggested previously. 相似文献