人喉癌Hep-2细胞膜钾离子通道与RNA编辑酶1的相关性

目的研究人喉癌细胞系Hep-2细胞膜钾离子通道特性及其与RNA编辑酶1(RNA-dependent adenosinedeaminase1,ADAR1)的相关性.方法以Hep-2细胞为研究对象,采用穿孔膜片钳全细胞记录法研究钾离子通道特性,用逆转录聚合酶链反应检测四乙胺(tetraethylammonium,TEA)阻断钾离子通道前后Hep-2细胞ADAR1 mRNA的表达.结果 Hep-2细胞膜的静息膜电位为(-29.8±1.9)mV,在钳制电压-40 mV,阶跃电压在-80～ 80 mV,记录到一种跨膜电流,该跨膜电流具有电压依赖、外向整流特性,该电流在延迟25ms后最大激活,800 ms内不失活,可被阻断剂TEA阻断.Hep-2细胞钾离子通道被TEA阻断前后ADAR1mRNA相对表达量存在显著性差异.结论 Hep-2细胞膜上存在延迟整流钾离子通道,此钾离子通道可能和ADAR1 mRNA的表达密切相关.     

罗库溴铵靶控输注用于老年患者腹部手术效果观察

目的:探讨罗库溴铵靶控输注(TCI)用于老年患者腹部手术的效果.方法:60例ASAⅡ级、择期全麻腹部手术老年患者(60～80岁),随机分成A、B、C 3组,每组20例.根据预实验结果,将A、B、C 3组诱导插管时罗库溴铵效应室靶浓度 (Ce) 均设置为3 mg/L,术中维持Ce分别为0.6、0.8、1.0 mg/L.观察各组诱导插管起效时间、气管插管条件、术中肌松条件和术后肌松恢复情况.结果:3组均可顺利完成气管插管;术中A组肌松条件满意率低于B组和C组.与B组比较,C组罗库溴铵用量大、术中肌肉阻滞程度较深、术后恢复时间延长(P<0.05).结论:老年患者腹部手术诱导插管时罗库溴铵TCI Ce设置为3 mg/L、术中Ce维持在0.8 mg/L,可以实现诱导插管顺利、术中肌松满意和术毕迅速恢复的目的.     

紫檀浸膏对5α-还原酶的抑制作用

目的 从中药中筛选5α-还原酶抑制物质,为开发治疗雄激素失调所致疾病的药物奠定基础.方法 将来自大鼠前列腺的5α-还原酶、丙酸睾酮及中药样品一起孵育,用EIA法测定DHT的生成量来反映酶的活性,筛选抑制酶活性的样品.同时,将所筛选样品用于治疗人工复制的前列腺肥大的大鼠模型,观察疗效.结果 紫檀的甲醇浸膏能抑制5α-还原酶,对人工复制的大鼠前列腺肥大有治疗作用.结论 所建筛选方法高效、可靠,所得紫檀浸膏具有进一步开发的价值.     

Tunable Boc modification of lignin and its impact on microbial degradation rate

A new type of modified lignin, lignin-p-Boc, was obtained through reaction with di-tert-butyl dicarbonate (Boc₂O) in aqueous media catalyzed by 4-dimethylaminopyridine (DMAP). Boc modification occurred regardless of type of lignin, was tunable, and proceeded well in recovering lignin at high purity from sodium lignosulfonate (a common byproduct from pulping industry; lignin content: 60%). Lignin-p-BOC was demonstrated as a potential reactive filler in green plastic and as a potential crosslinker in design of bioresorbable composite polymeric implants. Furthermore, the effect of the modification on breakdown rate of alkali lignin by microbes was investigated, and results showed that the modification substantially decreases the breakdown rate. The tunable Boc modification process was designed via a system thinking, including availability of raw lignin, economical/green modification, potentiality of drop-in-change to current thermoplastic processing, modification impact on microbial degradability/disposed environment at the end of use life; hence the holistic consideration makes this alternative method for upgrade of technical lignins very practical for future industrial application. Via “in-situ” forming “easily breakable covalent bonds” with existing thermopolymers inside, Lignin-p-BOCs are also promising to play an important role as both excellent binders via “random match” and reductants in transforming linear plastic waste into circular plastics.     

Resin Flow Analysis for the Foam Core Sandwich Spoiler by Vacuum-Assisted Resin Injection Process

This article presents the numerical analysis and experimental investigation for the manufacturing of a foam core sandwich spoiler by vacuum-assisted resin injection (VARI) process. To find an injection scheme that guarantees both a good impregnation of the preform and a filling time compatible with the process window, the finite element model (FEM) was applied to analyze the effect of different injection schemes on the resin flow front patterns. Based on the obtained results, two optimal injection schemes are selected to form the spoiler structure. The experimental results show that the best molding quality can be achieved from the thick-end injection with a thin-end exit scheme. The comparison between simulation and experimental results shows that the overall deviation of the numerical analysis on resin flow time is 15.9%.     

Intensity-modulated radiotherapy for cervical esophageal squamous cell carcinoma: clinical outcomes and patterns of failure

The objective of the study was to report clinical outcomes and patterns of failure for these patients with cervical esophageal squamous cell carcinoma (CESCC) treated with intensity-modulated radiotherapy (IMRT). A total of 64 patients with CESCC treated with definitive IMRT from May 2005 to March 2012 in our center were analyzed. Forty-two patients received radiotherapy alone and 22 patients received concurrent chemoradiotherapy. The location and extent of locoregional failures were transferred to the pretreatment planning computed tomography for dosimetry analysis. For all patients, the overall 2-year local failure-free survival, regional failure-free survival, distant failure-free survival, and overall survival rate was 74.5, 88.0, 66.6 and 42.5 %, respectively. Twenty-eight patients had developed treatment failure. Of the 28 patients, 14, 5, and 18 had developed local failure, regional failure, and distant metastasis, respectively. All of the 14 local failures were considered in-field failures. Of the five regional failures, three were considered in-field failures and two were marginal failures. The most frequently observed acute toxicity was mainly Grade 1 or 2. The incidence of acute Grade 3 mucositis (including pharyngitis), skin reaction, and leukopenia was 4.7, 12.5 and 10.9 %, respectively. IMRT provides satisfactory locoregional control for CESCC. Distant metastasis remains the predominate pattern of failure and the predominate pattern of locoregional failures is in-field failure.     

不同致敏变应原所致变应性鼻炎患者164例生活质量调查

目的:比较中国北方地区树木花粉、杂草花粉以及尘螨引起的变应性鼻炎(AR)患者的生活质量水平,伴随哮喘情况,探讨AR患者的生活质量与变应原血清特异性IgE的关系。方法:选取164例AR伴或不伴哮喘的成人患者,完成病史采集及变应原检查,并填写标准化鼻结膜炎生存质量调查问卷(RQLQ)。结果:中国北方地区AR患者中,树木花粉组及杂草花粉组的RQLQ总评分、日常活动评分、鼻部症状及眼部症状评分均显著高于尘螨组,生活质量更差。树木花粉组的哮喘比例明显低于尘螨组,而杂草花粉组的哮喘比例则与尘螨组无明显差异,且显著高于树木花粉组。尘螨及蒿属花粉过敏所致的AR患者的RQLQ评分与其变应原特异性IgE水平之间无明显相关性。结论:AR患者的生活质量评分受到变应原具体类型的影响,中国北方地区花粉过敏者发病时的生活质量比尘螨过敏者更差,但生活质量受损程度可能不受变应原特异性IgE水平的影响。不同花粉过敏所致鼻炎合并哮喘的比例不同,杂草花粉可能比树木花粉更容易诱发哮喘。     

榄香烯乳联合洛铂腹腔热灌注治疗中晚期结直肠癌恶性腹水的临床研究

目的 探讨榄香烯乳联合化疗药物洛铂腹腔热灌注对中晚期恶性结直肠癌腹水的临床疗效.方法 对2017年1月至2019年12月,广州医科大学附属肿瘤医院诊断为中晚期大肠癌并伴恶性腹水的患者共40例,按照入组条件,遵循抛硬币法分为治疗组(20例),对照组(20例),对照组予单药洛铂(50 mg),治疗组予洛铂(50 mg)加中...     

Comparison of the effects of perineural or intravenous dexamethasone on thoracic paravertebral block in Ivor‐Lewis esophagectomy: A double‐blind randomized trial

Efforts to prolong thoracic paravertebral block (TPVB) analgesia include local anesthetic adjuvants, such as dexamethasone (Dex). Previous studies showed that both perineural (PN) and intravenous (i.v.) routes could prolong analgesia. As PN Dex is an off‐label use, anesthesiologists should be fully informed of the clinical differences, if any, on block duration. This study was designed to evaluate the two administration routes of Dex for duration of analgesia in TPVB. Ninety‐five patients scheduled for Ivor‐Lewis esophagectomy were randomized to receive TPVB (0.5% ropivacaine 15 ml), PN or i.v. Dex 8 mg. The primary end point was the duration of analgesia. The secondary end points included pain scores, analgesic consumption, adverse effects rate, and incidence of chronic pain at 3 months postoperatively. The PN‐Dex group showed better analgesic effects than the i.v.‐Dex group (p < 0.05). Similarly, the visual analogue scale scores in patients at 2, 4, 8, and 12 h postoperatively were lower in the PN‐Dex group than the i.v.‐Dex group (p < 0.05). The analgesic consumption in both the PN‐Dex and i.v.‐Dex groups was significantly lower than that in the control group (p < 0.05). Regarding the incidence of chronic pain, regardless of route, Dex decreased the incidence of chronic postsurgical pain and neuropathic pain at 3 months after surgery (p < 0.05), but there were no clinical differences between the i.v.‐Dex and PN‐Dex groups. Perineural dexamethasone improved the magnitude and duration of analgesia compared to that of the i.v.‐Dex group in TPVB in Ivor‐Lewis esophagectomy. However, there were no clinically significant differences between the two groups in the incidence of chronic pain.

Study Highlights WHAT IS THE CURRENT KNOWLEDGE ON THE TOPIC? Both perineural (PN) and intravenous (i.v.) dexamethasone (Dex) could prolong the duration of a nerve block, but the superiority of either route is still inconclusive. WHAT QUESTION DID THIS STUDY ADDRESS? The study investigated the effects of the two routes of Dex added to ropivacaine on analgesic effects of thoracic paravertebral block in patients undergoing Ivor‐Lewis esophagectomy. WHAT DOES THIS STUDY ADD TO OUR KNOWLEDGE? These results extend the knowledge of the superior analgesic effect of Dex for the management of perioperative pain in the setting of Ivor‐Lewis Esophagectomy. HOW MIGHT THIS CHANGE CLINICAL PHARMACOLOGY OR TRANSLATIONAL SCIENCE? Because PN Dex is an off‐label use, our study conformed the safety of Dex as PN adjuvants and extended its application field in clinical work.