婴儿晚发性Vit K缺乏症的早期诊治及预防

目的：介绍婴儿晚发性vitK缺乏症早期诊治及预防的重要性。方法：选择186例婴儿晚发性vitK缺乏症患者,分析该病病因,临床特征,死亡率与就诊时间的关系。结果：24h之内就诊者24人,全部痊愈,－48h就诊者78例,死亡2例（占2．56％）,－72h就诊者36例,死亡12例（占33．33％）,超过72h就诊者48例,死亡23例（占58．3％）。结论：该病早期诊治能降低死亡率,而该病是可以预防的,提出对所有新生儿常规补充vitK以预防本病的发生。     

张謇的体育思想及其时代价值

张謇作为中国近代著名的实业家,也热衷于发展教育事业。张謇的体育思想,受当时一些主要社会思潮的影响,以“武备精神”为核心,以“体力与智力平均发达”为手段,以“德行艺全面发展”为灵魂,一贯主张体育与其它学科具有同等地位。张謇的体育思想,与当时时代主题相契合,明确体育课程的地位与价值,支持体育竞技活动的参与,高度重视公共体育场地设施建设,主张政府和学校共同致力于体育教育的大力发展,对当代中国的教育体育改革具有重要的借鉴意义。     

苣荬菜无机元素的分析

目的:分析苣荬菜中的无机元素.方法:采用美国Varian Spectra A30型原子吸收分光光度计测定.结果:表明苣荬菜中含有丰富的人体必需的无机元素.结论:苣荬菜有较高的开发利用价值.     

丁香苦苷对2型糖尿病小鼠降糖效果的实验研究

目的研究丁香苦苷对糖尿病小鼠模型的降糖作用,为临床寻求新的治疗糖尿病药物提供实验基础。方法选用C57BL遗传性肥胖T2DM动物模型(购自齐齐哈尔医学院动物实验中心)db/db小鼠80只(6~8周龄),随机分为模型组,正常对照组,低、中、高丁香苦苷剂量组,模型组和正常对照组采用生理盐水灌胃,剂量组采用12.5,25,50 mg/(kg·d)丁香苦苷灌胃,每天一次,连续4周,每周测量小鼠体质量。结果中、高剂量组体质量均大于模型组和低剂量组(P<0.05);空腹血糖与正常组比较,各模型组与正常组比较,差异均有统计学意义(P<0.05);中、高剂量组与低剂量和模型组相比,组间差异具有统计学意义(P<0.05)。糖耐量中高剂量组与其他组比较差异均有统计学意义(P<0.05);与模型组比较,一小时和两小时,中高剂量糖耐量值显著减少,差异有统计学意义(P<0.05)。结论中、高剂量丁香苦苷对糖尿病小鼠具有一定的降糖作用,为研究丁香苦苷临床疗效提供动物实验基础。     

血清药理实验方法学在抗鼻咽癌中药筛选中的应用

目的探讨血清药理实验方法学在筛选抗鼻咽癌中药方面的作用。方法将SD大鼠80只随机分为10组,实验组(分别灌胃肿节风、黄芪、薏苡仁、千斤藤、黑墨草、养阴清热解毒剂、复方天仙胶囊和鼻咽清毒颗粒)、阳性对照组(灌胃环磷酰胺)、阴性对照组(灌胃生理盐水),动物给药后采血,提取制备含药血清。运用MTT法检测作用药物血清后细胞增殖的改变,并用流式细胞仪(FCM)测定细胞周期的改变。结果肿节风、薏苡仁、复方天仙胶囊和鼻咽清毒颗粒四种中药可抑制CNE1细胞的增殖,其作用具有时间和浓度依赖性;同时可改变细胞周期分布,使多数细胞阻滞于G1期。而黄芪、黑墨草、千斤藤、养阴清热解毒剂无抗鼻咽癌作用。结论血清药理实验方法学是有效地筛选抗鼻咽癌中药的实验方法 ;肿节风、薏苡仁、复方天仙胶囊和鼻咽清毒颗粒是有效的抗鼻咽癌细胞生长的药物,而黄芪、黑墨草、千斤藤、养阴清热解毒剂无效。     

circ_0006089 promotes gastric cancer growth,metastasis, glycolysis,and angiogenesis by regulating miR‐361‐3p/TGFB1

Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed. Quantitative real‐time PCR was utilized for detecting circ_0006089, microRNA (miR)‐361‐3p and transforming growth factor‐β1 (TGFB1) expression. The interaction between miR‐361‐3p and circ_0006089 or TGFB1 was confirmed using a dual‐luciferase reporter assay and an RNA immunoprecipitation (RIP) assay. Cell proliferation, metastasis, apoptosis, and angiogenesis were determined using colony formation assay, EdU assay, transwell assay, flow cytometry, and tube formation assay. Cell glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP levels. In addition, western blot (WB) analysis was used to measure protein expression. Xenograft tumor models were used to assess the effect of circ_0006089 knockdown on GC tumorigenesis. circ_0006089 had been found to be upregulated in GC tissues and cells, and it could act as an miR‐361‐3p sponge. circ_0006089 knockdown suppressed GC proliferation, metastasis, glycolysis, angiogenesis, and increased apoptosis, while this effect could be revoked by miR‐361‐3p inhibitor. TGFB1 was targeted by miR‐361‐3p, and its overexpression reversed the effects of miR‐361‐3p on GC cell function. Also, circ_0006089 promoted TGFB1 expression via sponging miR‐361‐3p. Animal experiments showed that silenced circ_0006089 inhibited GC tumorigenesis through the miR‐361‐3p/TGFB1 pathway. Our results revealed that the circ_0006089/miR‐361‐3p/TGFB1 axis contributed to GC progression, confirming that circ_0006089 might be a potential therapeutic target for GC.     

Risk factors for and predictive nomogram of overall survival in adult patients with craniopharyngiomas: A SEER population-based study

Studies with relatively large sample size as well as long-term follow-up focusing on adult craniopharyngioma (CP) patients are still lacking. We attempted to identify independent prognostic factors and establish a nomogram model to estimate survival rates for adult CP patients.The Surveillance, Epidemiology, and End Results database was used to obtain data on patients with CP. Univariable and multivariable Cox analyses were utilized to identify the prognostic factors of adult CP patients. A survival prediction model was constructed and its predictive performance was also assessed.A total of 991 patients (695 in training group and 296 in validation group) were eligible for final inclusion. Multivariate Cox analysis presented that age at diagnosis, marital status, race, tumor size, and surgery type were statistically significant prognostic factors for overall survival (all P < .05). A graphical predicting nomogram model was developed to calculate the predicted patients’ survival probabilities at 1, 2, 5, and 10 years. The concordance indexes were 0.708 ± 0.019 and 0.750 ± 0.025 for the training and validation samples, respectively, demonstrating favorable discrimination abilities. Similarly, the time-dependent area under curve also showed overall satisfactory discrimination ability. Favorable consistencies between the predicted and actual survival were presented according to the calibration curves.An easy-to-use nomogram, being proven to be with reliable discrimination ability and accuracy, was established to help predict overall survival for adult patients with CP using the identified significant prognostic factors.     

Pan‐cancer analysis reveals sex‐specific signatures in the tumor microenvironment

The processes of cancer initiation, progression, and response to therapy are affected by the sex of cancer patients. Immunotherapy responses largely depend on the tumor microenvironment (TME), but how sex may shape some TME features, remains unknown. Here, we analyzed immune infiltration signatures across 19 cancer types from 1771 male and 1137 female patients in The Cancer Genome Atlas to evaluate how sex may affect the tumor mutational burden (TMB), immune scores, stromal scores, tumor purity, immune cells, immune checkpoint genes, and functional pathways in the TME. Pan‐cancer analyses showed higher TMB and tumor purity scores, as well as lower immune and stromal scores in male patients as compared to female patients. Lung adenocarcinoma, lung squamous carcinoma, kidney papillary carcinoma, and head and neck squamous carcinoma showed the most significant sex biases in terms of infiltrating immune cells, immune checkpoint gene expression, and functional pathways. We further focused on lung adenocarcinoma samples in order to identify and validate sex‐specific immune cell biomarkers with prognostic potential. Overall, sex may affect the tumor microenvironment, and sex‐specific TME biomarkers may help tailor cancer immunotherapy in certain cancer types.     

新医改后浙江省基层医务人员工作满意度调查

目的 分析浙江省基层医务人员在新医改后对工作的满意度情况,医院或相关行政部门可以相应地采取激励措施提高其工作积极性,更好地为患者提供高质量的健康服务.方法 在浙江省新医改试点医疗机构随机整群抽取266名医务人员进行调查,数据运用描述性统计方法进行比较分析.结果 新医改后近四成医务人员认为收入水平有所降低,但创收压力减少...