人乳头瘤病毒E6/E7 mRNA检测在宫颈癌筛查中的应用价值

目的 探讨人乳头瘤病毒(HPV)E6/E7 mRNA检测在宫颈癌筛查中的应用价值.方法 选取液基薄层细胞学检查(TCT)阳性的350例HPV感染患者,均接受HPV DNA检测和HPV E6/E7 mRNA检测,比较不同TBS分级、不同年龄段、不同病变程度HPV E6/E7 mRNA及HPV DNA的检出情况.以阴道镜病...     

Secondary bloodstream infection in critically ill patients with COVID-19

ObjectiveSecondary infection, especially bloodstream infection, is an important cause of death in critically ill patients with COVID-19. We aimed to describe secondary bloodstream infection (SBI) in critically ill adults with COVID-19 in the intensive care unit (ICU) and to explore risk factors related to SBI.MethodsWe reviewed all SBI cases among critically ill patients with COVID-19 from 12 February 2020 to 24 March 2020 in the COVID-19 ICU of Jingmen First People''s Hospital. We compared risk factors associated with bloodstream infection in this study. All SBIs were confirmed by blood culture.ResultsWe identified five cases of SBI among the 32 patients: three with Enterococcus faecium, one mixed septicemia (E. faecium and Candida albicans), and one C. parapsilosis. There were no significant differences between the SBI group and non-SBI group. Significant risk factors for SBI were extracorporeal membrane oxygenation, central venous catheter, indwelling urethral catheter, and nasogastric tube.ConclusionsOur findings confirmed that the incidence of secondary infection, particularly SBI, and mortality are high among critically ill patients with COVID-19. We showed that long-term hospitalization and invasive procedures such as tracheotomy, central venous catheter, indwelling urethral catheter, and nasogastric tube are risk factors for SBI and other complications.     

Vitapex用于乳牙根管充填的临床评价

目的探索适合乳牙根管充填治疗的材料.方法用两种根管充填材料对乳前牙进行根管治疗.实验组采用Vitapex糊剂充填,对照组采用常规碘仿氧化锌糊剂(简称碘仿糊剂)充填,均跟踪观察疗效.结果实验组平均有效率为93.4%,对照组平均有效率为69%.实验组疗效优于对照组(P<0.05).结论Vitapex糊剂适合作乳牙根管充填材料.     

赵玉庸教授治疗尿毒症验案2则

赵玉庸教授从事中医诊疗工作50余年,尤其擅长肾脏病的诊治,积累了丰富的临床经验.赵老认为尿毒症为本虚标实之证,本虚以脾肾衰败为主,标实为湿、浊、毒、瘀."肾络瘀阻"为病机关键.治疗宜健脾补肾、泄浊解毒通络,以降浊通络方治疗,中药辅以血液透析,对于部分尿毒症早期患者,及时中药介入,可逐步停止血透,稳定病情.     

控制院内感染护理作用探讨

目的 探讨护理部在医院感染工作中的作用。方法 自20012004年严抓消毒隔离，规范护理操作，强化科室制度，加强预防感染措施及消毒隔离效果监测。结果 2004年空气监测合格率为95．83％，工作物品染菌合格率为99．4％，消毒液浓度监测合格率为99．5％；消毒隔离制度执行及无菌技术操作合格率为97．S9％；一次性无菌物品使用后毁形率达100％；相关知识考试成绩人均平均95分以上。结论 建立健全规章制度，加强消毒技术规范培训及管理是预防医院感染的关键。     

Microsatellite instability in Chinese gastric cancer and its correlation with clinical characteristics

BackgroundMicrosatellite instability (MSI) remains a focus of interest in cancer research, but the characteristics of MSI in gastric cancer (GC) are ambiguous.MethodsIn this retrospective study, we analyzed the prevalence of MSI and the expression of programmed death-ligand 1 (PD-L1) and cluster of differentiation 8 (CD8) cells in Chinese GC patients. A total of 393 GC patients admitted to two centers from January 2010 to December 2017 were enrolled.ResultsThe prevalence of MSI in this cohort was 3.4% and most frequently occurred in females, patients aged between 59 and 69 years, and patients at a lower clinical stage. All MSI GCs had CD8 expression but lacked PD-L1 expression, indicating that MSI was related to CD8 expression but irrelevant to PD-L1 expression. However, there was no significant difference in the expression of CD8/PD-L1 between MSI GC and microsatellite stable (MSS) GC. Kaplan-Meier survival curves revealed that patients with MSI had a significantly longer overall survival (OS) than patients with MSS.ConclusionsIn Chinese GC patients, MSI frequently occurred in females, patients aged between 59 and 69, and patients with lower clinical stages. Patients with MSI-High (MSI-H) and MSI-Low (MSI-L) had a longer OS than patients with MSS. MSI was related to CD8 expression but irrelevant to PD-L1 expression.     

补肾化淤汤治疗强直性脊柱炎肾虚血瘀证的效果及对患者疾病活动程度、BASFI评分的影响

目的 探讨补肾化淤汤治疗强直性脊柱炎(AS)肾虚血瘀证的效果及对患者疾病活动程度、Bath强直性脊柱炎功能指数(BASFI)评分的影响.方法 选取2018年6月至2020年12月我院接收的86例AS肾虚血瘀证患者进行研究,以治疗方案将其分为对照组与观察组,各43例.对照组采取常规西药治疗,观察组在对照组治疗基础上加施补...     

2型糖尿病患者的心率减速力及其影响因素分析

目的观察2型糖尿病(T2DM)患者的心率减速力(DC)及其影响因素。方法选择本院2017年4月至2019年10月收诊的69例T2DM患者为研究对象。采用12导联动态心电记录仪监测和计算患者的DC值,收集C肽、HbA1c、FBG、LDL-C、HDL-C、TG、TC、PCT、IL-18、CRP水平的相关资料。按照DC值大小对患者进行分组(DC>4.5 ms,视作低危值;DC在2.5~4.5 ms,视作中危值;DC<2.5 ms,视作高危值)。比较不同组别患者的血糖、血脂、炎症因子水平,通过多因素Logistic回归分析观察影响T2DM患者DC值的因素。结果按照DC值不同将69例T2DM患者分为A组(低危值,24例)、B组(中危值,22例)、C组(高危值,23例)。单因素分析结果显示,三组的HbA1c、FBG、LDL-C、TG、TC、PCT、IL-18、CRP水平有显著差异,且A组相似文献   

Mechanistic and therapeutic advances in non-alcoholic fatty liver disease by targeting the gut microbiota

Non-alcoholic fatty liver disease (NAFLD) is one of the most common metabolic diseases currently in the context of obesity worldwide, which contains a spectrum of chronic liver diseases, including hepatic steatosis, non-alcoholic steatohepatitis and hepatic carcinoma. In addition to the classical “Two-hit” theory, NAFLD has been recognized as a typical gut microbiota-related disease because of the intricate role of gut microbiota in maintaining human health and disease formation. Moreover, gut microbiota is even regarded as a “metabolic organ” that play complementary roles to that of liver in many aspects. The mechanisms underlying gut microbiota-mediated development of NAFLD include modulation of host energy metabolism, insulin sensitivity, and bile acid and choline metabolism. As a result, gut microbiota have been emerging as a novel therapeutic target for NAFLD by manipulating it in various ways, including probiotics, prebiotics, synbiotics, antibiotics, fecal microbiota transplantation, and herbal components. In this review, we summarized the most recent advances in gut microbiota-mediated mechanisms, as well as gut microbiota-targeted therapies on NAFLD.     

肺癌上皮间质转化后引发Nrf2介导的多药耐药的研究

目的 探讨肺癌中上皮间质转化通过Nrf2通路导致多药耐药的可能性。方法 TGF-β1诱导肺腺癌细胞发生上皮间质转化, Western blot法检测癌细胞发生上皮间质转化的标志分子和细胞核内Nrf2的表达变化;比色法测定GSH含量及GST活力的变化;MTT实验检测癌细胞发生上皮间质转化前后对顺铂耐药指数的变化。结果 TGF-β1使癌细胞发生了上皮间质转化：细胞失去极性,上皮标志分子E-cadherin表达下降,而间质标志分子Vimentin和Snail表达上升;细胞发生上皮间质转化后细胞核内Nrf2表达显著增加,GSH含量增高及GST活力增强,癌细胞对顺铂的耐药指数是原来的7.7倍。结论 肺癌上皮间质转化后可能通过Nrf2通路介导了癌细胞的多药耐药。